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mmu_circDusp12_284
Basic information of circular RNA
PanCircBase ID:
mmu_circDusp12_284
mm39_Chr. Position ID:
chr1|170707228|170707795|-
Gene name:
Dusp12
Transcript:
ENSMUST00000170420.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
2
Exon/intron indexes:
4,3
Exon/intron offsets:
0,470
Genomic length:
567
Exon/intron sizes in nt:
187,97
Flanking exon/introns:
chr1:170702139-170707228|chr1:170707795-170708483
Splice length:
284
Other database IDs:
circAtlas: mmu-Dusp12_0004
circAtlas: hsa-SCAF8_0001, CIRCpedia: HSA_CIRCpedia_28479
Human Ortholog IDs:
Mature spliced Sequence:
AACTTTGGAATTTACCTCAGGAACTCTTCGCTGTTGACCCAACTACCATTTCACAGGGATTAAAAGATGACATTCTCTACAAATGCAGAAAATGCAGGCGGTCTTTATTTAGACATTCTAGTATTTTGGGTCATAGTGAAGGAAGTGGGCCAATAGCCTTTGCTCACAAGAGAACGGCGCCATCTTCTGTACTTACCACAGGGAGTCAGGCTCAGTGCACGTCTTACTTCATTGAGCCTGTGCAGTGGATGGAATCTACTCTGTTGGGCGTTATGGATGGACAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CTTACCACAGGGAGTCAGGC
59.75
GGTAGTTGGGTCAACAGCGA
59.97
140
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TTACCACAGGGAGTCAGGCT
60.18
TGGTAGTTGGGTCAACAGCG
60.25
140
PCR template sequence:
TTCTGTACTTACCACAGGGAGTCAGGCTCAGTGCACGTCTTACTTCATTGAGCCTGTGCAGTGGATGGAATCTACTCTGTTGGGCGTTATGGATGGACAGAACTTTGGAATTTACCTCAGGAACTCTTCGCTGTTGACCCAACTACCATTTCACAGGGATTAAAAGATGACATTCTCTACAAATGCAGAAAATGCAGGCG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
3
Interacting miRNAs (#binding sites)
mmu-miR-153-3p(1), mmu-miR-690(1), mmu-miR-484(1)
siRNAs for circular RNA silencing
Junction sequence
TATGGATGGACAGAACTTTGGAATTT
siRNA sets
AAATTCCAAAGTTCTGTCC, AATTCCAAAGTTCTGTCCA, TCCAAAGTTCTGTCCATCC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCDUSP12_284_ORF_1
0.6427
-0.062698033
0.026515511
99
ORF sequence:
ATGGAATCTACTCTGTTGGGCGTTATGGATGGACAGAACTTTGGAATTTACCTCAGGAACTCTTCGCTGTTGACCCAACTACCATTTCACAGGGATTAA
Peptide sequence:
MESTLLGVMDGQNFGIYLRNSSLLTQLPFHRD
IRESfinder result
IRESfinder score
0.730871397
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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