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mmu_circDst_501
Basic information of circular RNA
PanCircBase ID:
mmu_circDst_501
mm39_Chr. Position ID:
chr1|34237598|34238639|+
Gene name:
Dst
Transcript:
ENSMUST00000183034.5
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
2
Exon/intron indexes:
45,46
Exon/intron offsets:
0,774
Genomic length:
1041
Exon/intron sizes in nt:
2,34,267
Flanking exon/introns:
chr1:34236434-34237598|chr1:34238639-34239950
Splice length:
501
Other database IDs:
circAtlas: mmu-Dst_0077
circAtlas: hsa-SCAF8_0001
Human Ortholog IDs:
Mature spliced Sequence:
GAATTACAGAGAGATATGCAAGGAAGTACACAGGCAATGGAAGAAATAGTGCGAAACACAGAACTCTTTTTAAAAGAGAGCGGTGACGAGTTGTCCCAAGCGGATAGAGCTTTGATTGAGCAGAAACTGAACGAGGTGAAGATGAAGTGTGCGCAGCTGAACTTAAAGGCTGAGCAGTCGAGAAAGGAGTTGGATAAGGCAGTGACTACGGCCCTCAAGGAGGAGACTGAAAAGGTTGCAGCTGTGAGGCAGCTGGAAGAGAGCAAAACCAAGATAGAAAATCTTTTGAACTGGCTGTCAAATGTTGAGGAAGACTCCGAAGGGGTGTGGACGAAGCACACACAACCCATGGAGCAGAACGGAACCTATTTGCATGAAGGTGATAGCAAGTTAGGAGCTGGCGAAGAGGATGAAGTCAATGGTAACCTGCTGGAGACAGATGCTGAAGGGCACAGTGAGGCCACGAAGGGGAATCTGAACCAGCAGTATGAGAAAGTCAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AGACAGATGCTGAAGGGCAC
60.04
CAACTCGTCACCGCTCTCTT
60.04
161
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GAGACAGATGCTGAAGGGCA
59.75
CAACTCGTCACCGCTCTCTT
60.04
162
PCR template sequence:
CGAAGAGGATGAAGTCAATGGTAACCTGCTGGAGACAGATGCTGAAGGGCACAGTGAGGCCACGAAGGGGAATCTGAACCAGCAGTATGAGAAAGTCAAGGAATTACAGAGAGATATGCAAGGAAGTACACAGGCAATGGAAGAAATAGTGCGAAACACAGAACTCTTTTTAAAAGAGAGCGGTGACGAGTTGTCCCAAG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
3
Interacting miRNAs (#binding sites)
mmu-miR-218-5p(1), mmu-miR-673-5p(1), mmu-miR-1904(1)
siRNAs for circular RNA silencing
Junction sequence
TGAGAAAGTCAAGGAATTACAGAGAG
siRNA sets
TAATTCCTTGACTTTCTCA, CTCTCTGTAATTCCTTGAC, GTAATTCCTTGACTTTCTC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCDST_501_ORF_1
1.1401
0.367194513
0.995837468
987
ORF sequence:
ATGCAAGGAAGTACACAGGCAATGGAAGAAATAGTGCGAAACACAGAACTCTTTTTAAAAGAGAGCGGTGACGAGTTGTCCCAAGCGGATAGAGCTTTGATTGAGCAGAAACTGAACGAGGTGAAGATGAAGTGTGCGCAGCTGAACTTAAAGGCTGAGCAGTCGAGAAAGGAGTTGGATAAGGCAGTGACTACGGCCCTCAAGGAGGAGACTGAAAAGGTTGCAGCTGTGAGGCAGCTGGAAGAGAGCAAAACCAAGATAGAAAATCTTTTGAACTGGCTGTCAAATGTTGAGGAAGACTCCGAAGGGGTGTGGACGAAGCACACACAACCCATGGAGCAGAACGGAACCTATTTGCATGAAGGTGATAGCAAGTTAGGAGCTGGCGAAGAGGATGAAGTCAATGGTAACCTGCTGGAGACAGATGCTGAAGGGCACAGTGAGGCCACGAAGGGGAATCTGAACCAGCAGTATGAGAAAGTCAAGGAATTACAGAGAGATATGCAAGGAAGTACACAGGCAATGGAAGAAATAGTGCGAAACACAGAACTCTTTTTAAAAGAGAGCGGTGACGAGTTGTCCCAAGCGGATAGAGCTTTGATTGAGCAGAAACTGAACGAGGTGAAGATGAAGTGTGCGCAGCTGAACTTAAAGGCTGAGCAGTCGAGAAAGGAGTTGGATAAGGCAGTGACTACGGCCCTCAAGGAGGAGACTGAAAAGGTTGCAGCTGTGAGGCAGCTGGAAGAGAGCAAAACCAAGATAGAAAATCTTTTGAACTGGCTGTCAAATGTTGAGGAAGACTCCGAAGGGGTGTGGACGAAGCACACACAACCCATGGAGCAGAACGGAACCTATTTGCATGAAGGTGATAGCAAGTTAGGAGCTGGCGAAGAGGATGAAGTCAATGGTAACCTGCTGGAGACAGATGCTGAAGGGCACAGTGAGGCCACGAAGGGGAATCTGAACCAGCAGTATGAGAAAGTCAAG
Peptide sequence:
MQGSTQAMEEIVRNTELFLKESGDELSQADRALIEQKLNEVKMKCAQLNLKAEQSRKELDKAVTTALKEETEKVAAVRQLEESKTKIENLLNWLSNVEEDSEGVWTKHTQPMEQNGTYLHEGDSKLGAGEEDEVNGNLLETDAEGHSEATKGNLNQQYEKVKELQRDMQGSTQAMEEIVRNTELFLKESGDELSQADRALIEQKLNEVKMKCAQLNLKAEQSRKELDKAVTTALKEETEKVAAVRQLEESKTKIENLLNWLSNVEEDSEGVWTKHTQPMEQNGTYLHEGDSKLGAGEEDEVNGNLLETDAEGHSEATKGNLNQQYEKVK
IRESfinder result
IRESfinder score
0.298024464
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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