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mmu_circDst_2181
Basic information of circular RNA
PanCircBase ID:
mmu_circDst_2181
mm39_Chr. Position ID:
chr1|34299244|34314960|+
Gene name:
Dst
Transcript:
ENSMUST00000183034.5
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
13
Exon/intron indexes:
72,73,74,75,76,77,78,79,80,81,82,83,84
Exon/intron offsets:
0,1856,3100,4361,4586,6693,8065,8571,10664,11191,14004,15041,15509
Genomic length:
15716
Exon/intron sizes in nt:
20,42,32,22,71,23,10,20,00,00,00,00,00,00,00,00,00,00,000
Flanking exon/introns:
chr1:34297021-34299244|chr1:34314960-34317100
Splice length:
2181
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
CAACTTCGAGAACTGATAGCCGAGCACAAGCCACACATAGATAAGATGAACAAGACTGGGCCACAGCTATTGGAACTGAGCCCAAAGGAAGGCATTTATATCCAAGAAAAGTATGTGGCAGCCGACACCCTCTACAGTCAAATTAAAGAAGATGTCAAAAAGCGAGCTGTGGTTCTTGATGAGGCCATTTCTCAGTCCACCCAGTTTCATGACAAGATCGACCAGATCCTTGAGAGCCTGGAACGCATTGCGGAGCGTCTGCGGCAGCCGCCATCCATCTCCGCCGAGGTGGAGAAGATCAAGGAGCAGATTGGTGAAAATAAGAGCGTGTCCGTGGACATGGAGAAGCTGCAGCCGCTATACGAGACTCTGAGGCAGAGGGGAGAGGAGATGATCGCCAGATCCGAGGGCACTGAAAAAGACGTATCTGCCAGAGCTGTACAGGATAAGTTGGACCAAATGGTTTTCATCTGGGGAAGCATCCACACGCTGGTCGAAGAACGGGAAGCCAAACTGCTGGATGTGATGGAGCTAGCGGAGAAATTCTGGTGCGATCACATGTCGCTGGTCGTTACCATCAAAGACACTCAAGATTTCATCCGGGACCTGGAGGATCCCGGAATCGACCCCTCGGTCGTAAAGCAGCAGCAGGAAGCTGCAGAGGCAATCAGAGAGGAGATTGACGGCCTGCAGGAGGAGCTGGACATGGTCATCACGCTAGGCTCAGAGCTCATCGCTGCATGTGGGGAGCCTGACAAACCCATTGTCAAGAAGAGCATTGACGAGCTAAATTCAGCATGGGATTCTCTAAATAAAGCTTGGAAGGACCGGGTGGACAGACTGGAAGAGGCCATGCAAGCTGCCGTTCAGTACCAGGACGGATTGCAGGGAATATTCGACTGGGTTGACATCGCAGGCAACAAGTTAGCCACAATGTCTCCCATTGGAACAGACCTGGAGACCGTCAAGCAGCAGATTGAAGAGCTGAAGCAATTTAAGTCAGAAGCTTATCAACAACAGATAGAAATGGAAAGGCTGAACCATCAAGCAGAACTGTTGCTGAAGAAAGTAACAGAGGAGGCAGACAAGCACACAGTCCAGGACCCGCTGATGGAGCTGAAGTTGATCTGGGACAGCCTGGACGAGCGAATTGTCAGCAGACAGCACAAGCTCGAGGGTGCTCTCTTAGCACTGGGTCAGTTCCAGCACGCCCTGGACGAGCTCCTCGCATGGCTGACACACACCAAAGGCTTGCTAAGTGAACAGAAACCCGTTGGAGGAGACCCGAAAGCCATTGAGATTGAACTGGCTAAGCATCACGTGCTCCAAAACGACGTCTTAGCCCACCAGTCCACGGTAGAAGCCGTCAACAAAGCAGGAAATGATCTCATCGAGTCGAGTGAAGGCGAAGAAGCAAGCAACCTCCAGTACAAGCTCAGAATTCTCAATCAGCGCTGGCAGGATATTTTGGAAAAGACAGATCAAAGAAAGCAGCAGCTGGACAGCGCCTTGCGCCAGGCCAAAGGGTTCCACGGTGAAATCGAAGATTTGCAACAGTGGCTGACGGACACAGAGCGTCATCTCTTGGCATCTAAACCTCTGGGAGGTCTCCCAGAGACAGCCAAGGAGCAGCTTAACGCACATATGGAAGTCTGCACCGCCTTTGCCATCAAGGAAGAGACGTACAAGAGCCTGATGCTGAGGGGGCAGCAGATGCTTGCAAGGTGTCCCCGATCTGCGGAGACCAACATTGACCAAGACATCACCAACCTGAAAGAGAAGTGGGAATCCGTGAAGAGCAAACTCAACGAGAAGAAAACAAAGTTGGAGGAGGCCCTGCACTTGGCCATGAACTTCCACAACTCCCTCCAGGACTTCATCAACTGGCTTACCCAGGCTGAGCAAACTCTGAACGTGGCTTCTCGGCCAAGTCTTATCTTGGACACCATCTTGTTTCAAATTGATGAGCACAAGGTCTTTGCGAACGAAGTGAATTCTCACCGCGAGCAGATAATAGAGTTGGACAAGACTGGAACCCACCTGAAGTATTTCAGTCAGAAACAAGATGTCGTGCTCATCAAGAACCTGCTCATCAGCGTGCAGAGTCGGTGGGAAAAGGTGGTGCAGCGCCTGGTAGAGAGAGGGCGATCCCTGGACGAAGCCAGGAAGAGGGCCAAGCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TGCAGAGTCGGTGGGAAAAG
60.25
AGTTCCAATAGCTGTGGCCC
60.03
160
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TGCAGAGTCGGTGGGAAAAG
60.25
TTCCAATAGCTGTGGCCCAG
60.03
158
PCR template sequence:
GAACCTGCTCATCAGCGTGCAGAGTCGGTGGGAAAAGGTGGTGCAGCGCCTGGTAGAGAGAGGGCGATCCCTGGACGAAGCCAGGAAGAGGGCCAAGCAGCAACTTCGAGAACTGATAGCCGAGCACAAGCCACACATAGATAAGATGAACAAGACTGGGCCACAGCTATTGGAACTGAGCCCAAAGGAAGGCATTTATA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
16
Interacting miRNAs (#binding sites)
mmu-miR-210-3p(1), mmu-miR-9-5p(1), mmu-miR-328-3p(1), mmu-miR-216a-5p(1), mmu-miR-326-3p(1), mmu-miR-143-3p(1), mmu-miR-24-3p(1), mmu-miR-434-5p(1), mmu-miR-218-5p(1), mmu-miR-499-5p(1), mmu-miR-1187(1), mmu-miR-1198-5p(1), mmu-miR-335-3p(1), mmu-miR-188-3p(1), mmu-miR-338-3p(1), mmu-miR-542-5p(1)
siRNAs for circular RNA silencing
Junction sequence
GAGGGCCAAGCAGCAACTTCGAGAAC
siRNA sets
TTCTCGAAGTTGCTGCTTG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCDST_2181_ORF_1
1.3011
0.461341152
1
4317
ORF sequence:
ATGAACAAGACTGGGCCACAGCTATTGGAACTGAGCCCAAAGGAAGGCATTTATATCCAAGAAAAGTATGTGGCAGCCGACACCCTCTACAGTCAAATTAAAGAAGATGTCAAAAAGCGAGCTGTGGTTCTTGATGAGGCCATTTCTCAGTCCACCCAGTTTCATGACAAGATCGACCAGATCCTTGAGAGCCTGGAACGCATTGCGGAGCGTCTGCGGCAGCCGCCATCCATCTCCGCCGAGGTGGAGAAGATCAAGGAGCAGATTGGTGAAAATAAGAGCGTGTCCGTGGACATGGAGAAGCTGCAGCCGCTATACGAGACTCTGAGGCAGAGGGGAGAGGAGATGATCGCCAGATCCGAGGGCACTGAAAAAGACGTATCTGCCAGAGCTGTACAGGATAAGTTGGACCAAATGGTTTTCATCTGGGGAAGCATCCACACGCTGGTCGAAGAACGGGAAGCCAAACTGCTGGATGTGATGGAGCTAGCGGAGAAATTCTGGTGCGATCACATGTCGCTGGTCGTTACCATCAAAGACACTCAAGATTTCATCCGGGACCTGGAGGATCCCGGAATCGACCCCTCGGTCGTAAAGCAGCAGCAGGAAGCTGCAGAGGCAATCAGAGAGGAGATTGACGGCCTGCAGGAGGAGCTGGACATGGTCATCACGCTAGGCTCAGAGCTCATCGCTGCATGTGGGGAGCCTGACAAACCCATTGTCAAGAAGAGCATTGACGAGCTAAATTCAGCATGGGATTCTCTAAATAAAGCTTGGAAGGACCGGGTGGACAGACTGGAAGAGGCCATGCAAGCTGCCGTTCAGTACCAGGACGGATTGCAGGGAATATTCGACTGGGTTGACATCGCAGGCAACAAGTTAGCCACAATGTCTCCCATTGGAACAGACCTGGAGACCGTCAAGCAGCAGATTGAAGAGCTGAAGCAATTTAAGTCAGAAGCTTATCAACAACAGATAGAAATGGAAAGGCTGAACCATCAAGCAGAACTGTTGCTGAAGAAAGTAACAGAGGAGGCAGACAAGCACACAGTCCAGGACCCGCTGATGGAGCTGAAGTTGATCTGGGACAGCCTGGACGAGCGAATTGTCAGCAGACAGCACAAGCTCGAGGGTGCTCTCTTAGCACTGGGTCAGTTCCAGCACGCCCTGGACGAGCTCCTCGCATGGCTGACACACACCAAAGGCTTGCTAAGTGAACAGAAACCCGTTGGAGGAGACCCGAAAGCCATTGAGATTGAACTGGCTAAGCATCACGTGCTCCAAAACGACGTCTTAGCCCACCAGTCCACGGTAGAAGCCGTCAACAAAGCAGGAAATGATCTCATCGAGTCGAGTGAAGGCGAAGAAGCAAGCAACCTCCAGTACAAGCTCAGAATTCTCAATCAGCGCTGGCAGGATATTTTGGAAAAGACAGATCAAAGAAAGCAGCAGCTGGACAGCGCCTTGCGCCAGGCCAAAGGGTTCCACGGTGAAATCGAAGATTTGCAACAGTGGCTGACGGACACAGAGCGTCATCTCTTGGCATCTAAACCTCTGGGAGGTCTCCCAGAGACAGCCAAGGAGCAGCTTAACGCACATATGGAAGTCTGCACCGCCTTTGCCATCAAGGAAGAGACGTACAAGAGCCTGATGCTGAGGGGGCAGCAGATGCTTGCAAGGTGTCCCCGATCTGCGGAGACCAACATTGACCAAGACATCACCAACCTGAAAGAGAAGTGGGAATCCGTGAAGAGCAAACTCAACGAGAAGAAAACAAAGTTGGAGGAGGCCCTGCACTTGGCCATGAACTTCCACAACTCCCTCCAGGACTTCATCAACTGGCTTACCCAGGCTGAGCAAACTCTGAACGTGGCTTCTCGGCCAAGTCTTATCTTGGACACCATCTTGTTTCAAATTGATGAGCACAAGGTCTTTGCGAACGAAGTGAATTCTCACCGCGAGCAGATAATAGAGTTGGACAAGACTGGAACCCACCTGAAGTATTTCAGTCAGAAACAAGATGTCGTGCTCATCAAGAACCTGCTCATCAGCGTGCAGAGTCGGTGGGAAAAGGTGGTGCAGCGCCTGGTAGAGAGAGGGCGATCCCTGGACGAAGCCAGGAAGAGGGCCAAGCAGCAACTTCGAGAACTGATAGCCGAGCACAAGCCACACATAGATAAGATGAACAAGACTGGGCCACAGCTATTGGAACTGAGCCCAAAGGAAGGCATTTATATCCAAGAAAAGTATGTGGCAGCCGACACCCTCTACAGTCAAATTAAAGAAGATGTCAAAAAGCGAGCTGTGGTTCTTGATGAGGCCATTTCTCAGTCCACCCAGTTTCATGACAAGATCGACCAGATCCTTGAGAGCCTGGAACGCATTGCGGAGCGTCTGCGGCAGCCGCCATCCATCTCCGCCGAGGTGGAGAAGATCAAGGAGCAGATTGGTGAAAATAAGAGCGTGTCCGTGGACATGGAGAAGCTGCAGCCGCTATACGAGACTCTGAGGCAGAGGGGAGAGGAGATGATCGCCAGATCCGAGGGCACTGAAAAAGACGTATCTGCCAGAGCTGTACAGGATAAGTTGGACCAAATGGTTTTCATCTGGGGAAGCATCCACACGCTGGTCGAAGAACGGGAAGCCAAACTGCTGGATGTGATGGAGCTAGCGGAGAAATTCTGGTGCGATCACATGTCGCTGGTCGTTACCATCAAAGACACTCAAGATTTCATCCGGGACCTGGAGGATCCCGGAATCGACCCCTCGGTCGTAAAGCAGCAGCAGGAAGCTGCAGAGGCAATCAGAGAGGAGATTGACGGCCTGCAGGAGGAGCTGGACATGGTCATCACGCTAGGCTCAGAGCTCATCGCTGCATGTGGGGAGCCTGACAAACCCATTGTCAAGAAGAGCATTGACGAGCTAAATTCAGCATGGGATTCTCTAAATAAAGCTTGGAAGGACCGGGTGGACAGACTGGAAGAGGCCATGCAAGCTGCCGTTCAGTACCAGGACGGATTGCAGGGAATATTCGACTGGGTTGACATCGCAGGCAACAAGTTAGCCACAATGTCTCCCATTGGAACAGACCTGGAGACCGTCAAGCAGCAGATTGAAGAGCTGAAGCAATTTAAGTCAGAAGCTTATCAACAACAGATAGAAATGGAAAGGCTGAACCATCAAGCAGAACTGTTGCTGAAGAAAGTAACAGAGGAGGCAGACAAGCACACAGTCCAGGACCCGCTGATGGAGCTGAAGTTGATCTGGGACAGCCTGGACGAGCGAATTGTCAGCAGACAGCACAAGCTCGAGGGTGCTCTCTTAGCACTGGGTCAGTTCCAGCACGCCCTGGACGAGCTCCTCGCATGGCTGACACACACCAAAGGCTTGCTAAGTGAACAGAAACCCGTTGGAGGAGACCCGAAAGCCATTGAGATTGAACTGGCTAAGCATCACGTGCTCCAAAACGACGTCTTAGCCCACCAGTCCACGGTAGAAGCCGTCAACAAAGCAGGAAATGATCTCATCGAGTCGAGTGAAGGCGAAGAAGCAAGCAACCTCCAGTACAAGCTCAGAATTCTCAATCAGCGCTGGCAGGATATTTTGGAAAAGACAGATCAAAGAAAGCAGCAGCTGGACAGCGCCTTGCGCCAGGCCAAAGGGTTCCACGGTGAAATCGAAGATTTGCAACAGTGGCTGACGGACACAGAGCGTCATCTCTTGGCATCTAAACCTCTGGGAGGTCTCCCAGAGACAGCCAAGGAGCAGCTTAACGCACATATGGAAGTCTGCACCGCCTTTGCCATCAAGGAAGAGACGTACAAGAGCCTGATGCTGAGGGGGCAGCAGATGCTTGCAAGGTGTCCCCGATCTGCGGAGACCAACATTGACCAAGACATCACCAACCTGAAAGAGAAGTGGGAATCCGTGAAGAGCAAACTCAACGAGAAGAAAACAAAGTTGGAGGAGGCCCTGCACTTGGCCATGAACTTCCACAACTCCCTCCAGGACTTCATCAACTGGCTTACCCAGGCTGAGCAAACTCTGAACGTGGCTTCTCGGCCAAGTCTTATCTTGGACACCATCTTGTTTCAAATTGATGAGCACAAGGTCTTTGCGAACGAAGTGAATTCTCACCGCGAGCAGATAATAGAGTTGGACAAGACTGGAACCCACCTGAAGTATTTCAGTCAGAAACAAGATGTCGTGCTCATCAAGAACCTGCTCATCAGCGTGCAGAGTCGGTGGGAAAAGGTGGTGCAGCGCCTGGTAGAGAGAGGGCGATCCCTGGACGAAGCCAGGAAGAGGGCCAAGCAG
Peptide sequence:
MNKTGPQLLELSPKEGIYIQEKYVAADTLYSQIKEDVKKRAVVLDEAISQSTQFHDKIDQILESLERIAERLRQPPSISAEVEKIKEQIGENKSVSVDMEKLQPLYETLRQRGEEMIARSEGTEKDVSARAVQDKLDQMVFIWGSIHTLVEEREAKLLDVMELAEKFWCDHMSLVVTIKDTQDFIRDLEDPGIDPSVVKQQQEAAEAIREEIDGLQEELDMVITLGSELIAACGEPDKPIVKKSIDELNSAWDSLNKAWKDRVDRLEEAMQAAVQYQDGLQGIFDWVDIAGNKLATMSPIGTDLETVKQQIEELKQFKSEAYQQQIEMERLNHQAELLLKKVTEEADKHTVQDPLMELKLIWDSLDERIVSRQHKLEGALLALGQFQHALDELLAWLTHTKGLLSEQKPVGGDPKAIEIELAKHHVLQNDVLAHQSTVEAVNKAGNDLIESSEGEEASNLQYKLRILNQRWQDILEKTDQRKQQLDSALRQAKGFHGEIEDLQQWLTDTERHLLASKPLGGLPETAKEQLNAHMEVCTAFAIKEETYKSLMLRGQQMLARCPRSAETNIDQDITNLKEKWESVKSKLNEKKTKLEEALHLAMNFHNSLQDFINWLTQAEQTLNVASRPSLILDTILFQIDEHKVFANEVNSHREQIIELDKTGTHLKYFSQKQDVVLIKNLLISVQSRWEKVVQRLVERGRSLDEARKRAKQQLRELIAEHKPHIDKMNKTGPQLLELSPKEGIYIQEKYVAADTLYSQIKEDVKKRAVVLDEAISQSTQFHDKIDQILESLERIAERLRQPPSISAEVEKIKEQIGENKSVSVDMEKLQPLYETLRQRGEEMIARSEGTEKDVSARAVQDKLDQMVFIWGSIHTLVEEREAKLLDVMELAEKFWCDHMSLVVTIKDTQDFIRDLEDPGIDPSVVKQQQEAAEAIREEIDGLQEELDMVITLGSELIAACGEPDKPIVKKSIDELNSAWDSLNKAWKDRVDRLEEAMQAAVQYQDGLQGIFDWVDIAGNKLATMSPIGTDLETVKQQIEELKQFKSEAYQQQIEMERLNHQAELLLKKVTEEADKHTVQDPLMELKLIWDSLDERIVSRQHKLEGALLALGQFQHALDELLAWLTHTKGLLSEQKPVGGDPKAIEIELAKHHVLQNDVLAHQSTVEAVNKAGNDLIESSEGEEASNLQYKLRILNQRWQDILEKTDQRKQQLDSALRQAKGFHGEIEDLQQWLTDTERHLLASKPLGGLPETAKEQLNAHMEVCTAFAIKEETYKSLMLRGQQMLARCPRSAETNIDQDITNLKEKWESVKSKLNEKKTKLEEALHLAMNFHNSLQDFINWLTQAEQTLNVASRPSLILDTILFQIDEHKVFANEVNSHREQIIELDKTGTHLKYFSQKQDVVLIKNLLISVQSRWEKVVQRLVERGRSLDEARKRAKQ
IRESfinder result
IRESfinder score
0.316887557
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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