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mmu_circDrg1_592
Basic information of circular RNA
PanCircBase ID:
mmu_circDrg1_592
mm39_Chr. Position ID:
chr11|3202159|3206712|-
Gene name:
Drg1
Transcript:
ENSMUST00000020741.12
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
4
Exon/intron indexes:
8,7,6,5
Exon/intron offsets:
0,484,2352,4383
Genomic length:
4553
Exon/intron sizes in nt:
123,168,131,170
Flanking exon/introns:
chr11:3200365-3202159|chr11:3206712-3209305
Splice length:
592
Other database IDs:
circBase: hsa_circ_0062900, circAtlas: hsa-DRG1_0002, CIRCpedia: HSA_CIRCpedia_36388
Human Ortholog IDs:
Mature spliced Sequence:
TGGCCCGAACGTGTAATTTGATTTTGATTGTTCTGGATGTCCTAAAACCCTTGGGACATAAGAAGATAATTGAAAATGAGTTGGAAGGCTTTGGGATTCGCTTGAACAGCAAACCCCCCAACATTGGCTTTAAGAAGAAAGACAAGGGAGGCATTAACCTCACAGCCACTTGTCCTCAGAGTGAACTGGATGCTGAAACAGTGAAGAGCATTCTGGCTGAATATAAGATTCATAATGCTGATGTGACTTTGCGTAGTGATGCCACAGCTGACGACCTCATTGACGTGGTCGAAGGGAACAGAGTTTATATTCCCTGTATCTATGTATTAAATAAGATTGATCAGATCTCCATTGAGGAGTTGGATATTATCTATAAGGTGCCTCACTGTGTACCCATCTCTGCTCATCACCGCTGGAATTTTGATGACCTTTTGGAGAAAATCTGGGACTATCTGAAACTAGTGAGAATTTACACCAAACCGAAAGGCCAGTTACCAGATTACACATCTCCAGTAGTGCTGCCTTATTCTAGGACCACTGTGGAAGATTTCTGCATGAAGATTCACAAAAATCTCATCAAAGAATTTAAATA
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
ACATCTCCAGTAGTGCTGCC
59.46
CCCAAGGGTTTTAGGACATCCA
59.96
144
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
ACATCTCCAGTAGTGCTGCC
59.46
TCCCAAGGGTTTTAGGACATCC
59.69
145
PCR template sequence:
TACCAGATTACACATCTCCAGTAGTGCTGCCTTATTCTAGGACCACTGTGGAAGATTTCTGCATGAAGATTCACAAAAATCTCATCAAAGAATTTAAATATGGCCCGAACGTGTAATTTGATTTTGATTGTTCTGGATGTCCTAAAACCCTTGGGACATAAGAAGATAATTGAAAATGAGTTGGAAGGCTTTGGGATTCG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
4
Interacting miRNAs (#binding sites)
mmu-miR-29b-1-5p(1), mmu-miR-466d-3p(1), mmu-miR-29b-2-5p(1), mmu-miR-3470b(1)
siRNAs for circular RNA silencing
Junction sequence
AAGAATTTAAATATGGCCCGAACGTG
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCDRG1_592_ORF_1
1.154
0.217165634
0.883705389
591
ORF sequence:
ATGGCCCGAACGTGTAATTTGATTTTGATTGTTCTGGATGTCCTAAAACCCTTGGGACATAAGAAGATAATTGAAAATGAGTTGGAAGGCTTTGGGATTCGCTTGAACAGCAAACCCCCCAACATTGGCTTTAAGAAGAAAGACAAGGGAGGCATTAACCTCACAGCCACTTGTCCTCAGAGTGAACTGGATGCTGAAACAGTGAAGAGCATTCTGGCTGAATATAAGATTCATAATGCTGATGTGACTTTGCGTAGTGATGCCACAGCTGACGACCTCATTGACGTGGTCGAAGGGAACAGAGTTTATATTCCCTGTATCTATGTATTAAATAAGATTGATCAGATCTCCATTGAGGAGTTGGATATTATCTATAAGGTGCCTCACTGTGTACCCATCTCTGCTCATCACCGCTGGAATTTTGATGACCTTTTGGAGAAAATCTGGGACTATCTGAAACTAGTGAGAATTTACACCAAACCGAAAGGCCAGTTACCAGATTACACATCTCCAGTAGTGCTGCCTTATTCTAGGACCACTGTGGAAGATTTCTGCATGAAGATTCACAAAAATCTCATCAAAGAATTTAAA
Peptide sequence:
MARTCNLILIVLDVLKPLGHKKIIENELEGFGIRLNSKPPNIGFKKKDKGGINLTATCPQSELDAETVKSILAEYKIHNADVTLRSDATADDLIDVVEGNRVYIPCIYVLNKIDQISIEELDIIYKVPHCVPISAHHRWNFDDLLEKIWDYLKLVRIYTKPKGQLPDYTSPVVLPYSRTTVEDFCMKIHKNLIKEFK
IRESfinder result
IRESfinder score
0.704116478
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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