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mmu_circDrc1_645
Basic information of circular RNA
PanCircBase ID:
mmu_circDrc1_645
mm39_Chr. Position ID:
chr5|30500011|30507867|+
Gene name:
Drc1
Transcript:
ENSMUST00000101448.5
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
5
Exon/intron indexes:
3,4,5,6,7
Exon/intron offsets:
0,2823,4388,5995,7733
Genomic length:
7856
Exon/intron sizes in nt:
113,184,138,87,123
Flanking exon/introns:
chr5:30499095-30500011|chr5:30507867-30512482
Splice length:
645
Other database IDs:
circAtlas: mmu-Drc1_0001
circBase: hsa_circ_0119644, circAtlas: hsa-DRC1_0001, CIRCpedia: HSA_CIRCpedia_122260
Human Ortholog IDs:
Mature spliced Sequence:
AAACTGACTAAACTCCTGCTCTGCGGGACCGAGTTGGTGACAAATATTCAGGTGGCTGCAGACGTCAGAGAGATCCACAGGAGGGTTGAAGAGGAGGAGACAAAGCGGCAGAGACTGGAGAAGCTGGAGAATGAAGTTAAGACCAGCCAGGATAAATTTGATGAAATCACGGCGAAGTGGGAGGAGGGCAGACGGAAGCGGATTCCCCAGGAACTGTGGGAGATGCTCAACAGCCAGCAGGTGCACTGTGCTGAGCTCATAGAAGACAAGAACAAGCTGGCCAACGAGCTGCAGCAGGAGTTAAAAATAAAGGATGACCAGTATGTGAAGGACTTGAAGAAACAGTCGGAAGACATCACCCTGATTCTGGAGAGAATGGAGGAGCAGGTGAAGAATGTGATGAAGAACTTCCGCCAGGAGCTGATTCACATCGAGAAAGCCTTTGAGTCAGAACGGCAAGAACTGCTATCCAGCAATAAGAAGAAATGGGAGCGAGCCCTGCAGGCTCACAATGCCAAGGAGCTGGAGTACCTCACCAACCGCATGAAGAAGGTGGAGGACTACGAGAAGCAGCTCAACAAGCAGCGGGTCTGGGACTGTGAGGAGTACAACACAATCAAGATCAAGCTAGAGCAGGATGTGCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GGAGGACTACGAGAAGCAGC
59.9
CGTCTGCAGCCACCTGAATA
60.11
155
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GGAGGACTACGAGAAGCAGC
59.9
ATCTCTCTGACGTCTGCAGC
59.54
165
PCR template sequence:
GAAGAAGGTGGAGGACTACGAGAAGCAGCTCAACAAGCAGCGGGTCTGGGACTGTGAGGAGTACAACACAATCAAGATCAAGCTAGAGCAGGATGTGCAGAAACTGACTAAACTCCTGCTCTGCGGGACCGAGTTGGTGACAAATATTCAGGTGGCTGCAGACGTCAGAGAGATCCACAGGAGGGTTGAAGAGGAGGAGA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
7
Interacting miRNAs (#binding sites)
mmu-miR-205-5p(1), mmu-miR-128-3p(1), mmu-miR-136-5p(1), mmu-miR-370-3p(1), mmu-miR-207(1), mmu-miR-452-3p(1), mmu-miR-697(1)
siRNAs for circular RNA silencing
Junction sequence
GCAGGATGTGCAGAAACTGACTAAAC
siRNA sets
TTTAGTCAGTTTCTGCACA, CAGTTTCTGCACATCCTGC, GTTTAGTCAGTTTCTGCAC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCDRC1_645_ORF_1
1.213
0.676273543
0.999421505
1068
ORF sequence:
ATGCTCAACAGCCAGCAGGTGCACTGTGCTGAGCTCATAGAAGACAAGAACAAGCTGGCCAACGAGCTGCAGCAGGAGTTAAAAATAAAGGATGACCAGTATGTGAAGGACTTGAAGAAACAGTCGGAAGACATCACCCTGATTCTGGAGAGAATGGAGGAGCAGGTGAAGAATGTGATGAAGAACTTCCGCCAGGAGCTGATTCACATCGAGAAAGCCTTTGAGTCAGAACGGCAAGAACTGCTATCCAGCAATAAGAAGAAATGGGAGCGAGCCCTGCAGGCTCACAATGCCAAGGAGCTGGAGTACCTCACCAACCGCATGAAGAAGGTGGAGGACTACGAGAAGCAGCTCAACAAGCAGCGGGTCTGGGACTGTGAGGAGTACAACACAATCAAGATCAAGCTAGAGCAGGATGTGCAGAAACTGACTAAACTCCTGCTCTGCGGGACCGAGTTGGTGACAAATATTCAGGTGGCTGCAGACGTCAGAGAGATCCACAGGAGGGTTGAAGAGGAGGAGACAAAGCGGCAGAGACTGGAGAAGCTGGAGAATGAAGTTAAGACCAGCCAGGATAAATTTGATGAAATCACGGCGAAGTGGGAGGAGGGCAGACGGAAGCGGATTCCCCAGGAACTGTGGGAGATGCTCAACAGCCAGCAGGTGCACTGTGCTGAGCTCATAGAAGACAAGAACAAGCTGGCCAACGAGCTGCAGCAGGAGTTAAAAATAAAGGATGACCAGTATGTGAAGGACTTGAAGAAACAGTCGGAAGACATCACCCTGATTCTGGAGAGAATGGAGGAGCAGGTGAAGAATGTGATGAAGAACTTCCGCCAGGAGCTGATTCACATCGAGAAAGCCTTTGAGTCAGAACGGCAAGAACTGCTATCCAGCAATAAGAAGAAATGGGAGCGAGCCCTGCAGGCTCACAATGCCAAGGAGCTGGAGTACCTCACCAACCGCATGAAGAAGGTGGAGGACTACGAGAAGCAGCTCAACAAGCAGCGGGTCTGGGACTGTGAGGAGTACAACACAATCAAGATCAAGCTAGAGCAGGATGTGCAG
Peptide sequence:
MLNSQQVHCAELIEDKNKLANELQQELKIKDDQYVKDLKKQSEDITLILERMEEQVKNVMKNFRQELIHIEKAFESERQELLSSNKKKWERALQAHNAKELEYLTNRMKKVEDYEKQLNKQRVWDCEEYNTIKIKLEQDVQKLTKLLLCGTELVTNIQVAADVREIHRRVEEEETKRQRLEKLENEVKTSQDKFDEITAKWEEGRRKRIPQELWEMLNSQQVHCAELIEDKNKLANELQQELKIKDDQYVKDLKKQSEDITLILERMEEQVKNVMKNFRQELIHIEKAFESERQELLSSNKKKWERALQAHNAKELEYLTNRMKKVEDYEKQLNKQRVWDCEEYNTIKIKLEQDVQ
IRESfinder result
IRESfinder score
0.250467282
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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