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mmu_circDpp8_735
Basic information of circular RNA
PanCircBase ID:
mmu_circDpp8_735
mm39_Chr. Position ID:
chr9|64970708|64983170|+
Gene name:
Dpp8
Transcript:
ENSMUST00000034960.14
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
5
Exon/intron indexes:
14,15,16,17,18
Exon/intron offsets:
0,392,2924,11012,12309
Genomic length:
12462
Exon/intron sizes in nt:
15,31,36,14,61,47,153
Flanking exon/introns:
chr9:64967564-64970708|chr9:64983170-64985244
Splice length:
735
Other database IDs:
circAtlas: hsa-DPP8_0036, CIRCpedia: HSA_CIRCpedia_15233
Human Ortholog IDs:
Mature spliced Sequence:
ATCTGGGTTGATGAAGCCAGAAAGCTGGTGTACTTTGAAGGCACCAAAGACTCTCCTTTGGAGCATCACCTGTACGTGACCAGTTATGCAAACCCTGGAGAAGTGGTGAGGCTGACTGACCGTGGCTACTCACACTCCTGCTGCCTCAGCCGGCATTGTGACTTCTTCATAAGTAAGTACAGCAACCAGAAGAATCCACACTGTGTGTCCCTCTACAAACTCTCAAGTCCTGAGGATGACCCAGTTCATAAAACAAAGGAATTTTGGGCCACCATTTTGGATTCAGCAGGTCCTCTTCCTGACTACACCCCTCCAGAAATTTTTTCTTTTGAAAGTACTACTGGATTTACACTGTATGGAATGTTGTATAAGCCTCATGACCTACAACCTGGAAAGAAATACCCCACTGTGTTATTCATATATGGTGGTCCCCAGGTGCAGCTGGTGAACAATCGGTTTAAAGGAGTCAAGTATTTCCGCCTGAACACCCTGGCCTCCCTGGGTTATGTGGTTGTGGTGATAGACAACAGGGGATCCTGTCACCGAGGACTTAAATTTGAAGGCGCCTTTAAATATAAAATGGGTCAAATAGAAATCGATGATCAAGTGGAAGGACTCCAGTACCTAGCATCTCAGTATGACTTCATTGACTTGGATCGAGTGGGCATCCACGGCTGGTCCTATGGTGGCTACCTCTCCCTGATGGCATTAATGCAGAGGTCGGATATCTTCCGG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TTGACTTGGATCGAGTGGGC
60.04
CACGTACAGGTGATGCTCCA
59.75
167
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GCTGGTCCTATGGTGGCTAC
59.89
CACGTACAGGTGATGCTCCA
59.75
140
PCR template sequence:
GTATGACTTCATTGACTTGGATCGAGTGGGCATCCACGGCTGGTCCTATGGTGGCTACCTCTCCCTGATGGCATTAATGCAGAGGTCGGATATCTTCCGGATCTGGGTTGATGAAGCCAGAAAGCTGGTGTACTTTGAAGGCACCAAAGACTCTCCTTTGGAGCATCACCTGTACGTGACCAGTTATGCAAACCCTGGAG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
5
Interacting miRNAs (#binding sites)
mmu-let-7b-5p(1), mmu-miR-122-5p(1), mmu-miR-709(1), mmu-miR-207(1), mmu-miR-574-5p(1)
siRNAs for circular RNA silencing
Junction sequence
GGATATCTTCCGGATCTGGGTTGATG
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCDPP8_735_ORF_1
0.9385
0.231379499
0.995609585
1110
ORF sequence:
ATGTTGTATAAGCCTCATGACCTACAACCTGGAAAGAAATACCCCACTGTGTTATTCATATATGGTGGTCCCCAGGTGCAGCTGGTGAACAATCGGTTTAAAGGAGTCAAGTATTTCCGCCTGAACACCCTGGCCTCCCTGGGTTATGTGGTTGTGGTGATAGACAACAGGGGATCCTGTCACCGAGGACTTAAATTTGAAGGCGCCTTTAAATATAAAATGGGTCAAATAGAAATCGATGATCAAGTGGAAGGACTCCAGTACCTAGCATCTCAGTATGACTTCATTGACTTGGATCGAGTGGGCATCCACGGCTGGTCCTATGGTGGCTACCTCTCCCTGATGGCATTAATGCAGAGGTCGGATATCTTCCGGATCTGGGTTGATGAAGCCAGAAAGCTGGTGTACTTTGAAGGCACCAAAGACTCTCCTTTGGAGCATCACCTGTACGTGACCAGTTATGCAAACCCTGGAGAAGTGGTGAGGCTGACTGACCGTGGCTACTCACACTCCTGCTGCCTCAGCCGGCATTGTGACTTCTTCATAAGTAAGTACAGCAACCAGAAGAATCCACACTGTGTGTCCCTCTACAAACTCTCAAGTCCTGAGGATGACCCAGTTCATAAAACAAAGGAATTTTGGGCCACCATTTTGGATTCAGCAGGTCCTCTTCCTGACTACACCCCTCCAGAAATTTTTTCTTTTGAAAGTACTACTGGATTTACACTGTATGGAATGTTGTATAAGCCTCATGACCTACAACCTGGAAAGAAATACCCCACTGTGTTATTCATATATGGTGGTCCCCAGGTGCAGCTGGTGAACAATCGGTTTAAAGGAGTCAAGTATTTCCGCCTGAACACCCTGGCCTCCCTGGGTTATGTGGTTGTGGTGATAGACAACAGGGGATCCTGTCACCGAGGACTTAAATTTGAAGGCGCCTTTAAATATAAAATGGGTCAAATAGAAATCGATGATCAAGTGGAAGGACTCCAGTACCTAGCATCTCAGTATGACTTCATTGACTTGGATCGAGTGGGCATCCACGGCTGGTCCTATGGTGGCTACCTCTCCCTGATGGCATTAATGCAGAGGTCGGATATCTTCCGG
Peptide sequence:
MLYKPHDLQPGKKYPTVLFIYGGPQVQLVNNRFKGVKYFRLNTLASLGYVVVVIDNRGSCHRGLKFEGAFKYKMGQIEIDDQVEGLQYLASQYDFIDLDRVGIHGWSYGGYLSLMALMQRSDIFRIWVDEARKLVYFEGTKDSPLEHHLYVTSYANPGEVVRLTDRGYSHSCCLSRHCDFFISKYSNQKNPHCVSLYKLSSPEDDPVHKTKEFWATILDSAGPLPDYTPPEIFSFESTTGFTLYGMLYKPHDLQPGKKYPTVLFIYGGPQVQLVNNRFKGVKYFRLNTLASLGYVVVVIDNRGSCHRGLKFEGAFKYKMGQIEIDDQVEGLQYLASQYDFIDLDRVGIHGWSYGGYLSLMALMQRSDIFR
IRESfinder result
IRESfinder score
0.600710431
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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