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mmu_circDpp4_689
Basic information of circular RNA
PanCircBase ID:
mmu_circDpp4_689
mm39_Chr. Position ID:
chr2|62178161|62189249|-
Gene name:
Dpp4
Transcript:
ENSMUST00000047812.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
7
Exon/intron indexes:
22,21,20,19,18,17,16
Exon/intron offsets:
0,683,4304,6721,8798,9142,10966
Genomic length:
11088
Exon/intron sizes in nt:
10,05,51,95,70,99,48,100
Flanking exon/introns:
chr2:62175464-62178161|chr2:62189249-62189620
Splice length:
689
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
AATTCAACTTACTGACCACACAAATGTGAAGTGCCTTAGTTGTGACCTGAATCCAGAAAGATGTCAGTATTATGCGGTATCATTTAGTAAAGAGGCAAAGTACTATCAGCTGGGATGTTGGGGCCCCGGTCTGCCCCTCTACACTCTACATCGTAGCACGGATCATAAAGAGCTGCGAGTCCTGGAAGACAATTCTGCTTTGGATAGAATGCTGCAGGATGTCCAGATGCCTTCAAAAAAATTGGACTTCATTGTTTTGAATGAAACAAGATTTTGGTATCAAATGATCTTGCCCCCTCATTTTGATAAATCCAAGAAATATCCTCTACTATTAGATGTATATGCAGGTCCCTGTAGTCAAAAAGCAGATGCTTCCTTCAGACTGAACTGGGCCACTTACCTTGCAAGTACAGAAAACATCATAGTAGCTAGCTTTGACGGCAGAGGAAGTGGTTACCAAGGAGATAAGATCATGCATGCAATCAACAGAAGATTGGGAACACTGGAAGTTGAAGATCAAATTGAAGCAGCCAGGCAATTTGTAAAAATGGGATTTGTGGATAGCAAGCGAGTTGCAATTTGGGGCTGGTCATATGGAGGGTATGTAACCTCAATGGTCCTGGGATCGGGAAGTGGCGTGTTCAAGTGCGGAATAGCTGTGGCACCTGTGTCACGGTGGGAGTACTATG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CTCAATGGTCCTGGGATCGG
59.89
ACATCTTTCTGGATTCAGGTCACA
59.96
144
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CCTCAATGGTCCTGGGATCG
59.89
ACATCTTTCTGGATTCAGGTCACA
59.96
145
PCR template sequence:
TCATATGGAGGGTATGTAACCTCAATGGTCCTGGGATCGGGAAGTGGCGTGTTCAAGTGCGGAATAGCTGTGGCACCTGTGTCACGGTGGGAGTACTATGAATTCAACTTACTGACCACACAAATGTGAAGTGCCTTAGTTGTGACCTGAATCCAGAAAGATGTCAGTATTATGCGGTATCATTTAGTAAAGAGGCAAAG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
8
Interacting miRNAs (#binding sites)
mmu-miR-199a-5p(1), mmu-miR-103-3p(1), mmu-miR-31-5p(1), mmu-miR-24-3p(1), mmu-miR-107-3p(1), mmu-miR-680(1), mmu-miR-369-3p(1), mmu-miR-466b-3p(1)
siRNAs for circular RNA silencing
Junction sequence
TGGGAGTACTATGAATTCAACTTACT
siRNA sets
TTGAATTCATAGTACTCCC, TGAATTCATAGTACTCCCA, AAGTTGAATTCATAGTACT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCDPP4_689_ORF_1
0.7261
0.013195032
0.452450573
510
ORF sequence:
ATGCTGCAGGATGTCCAGATGCCTTCAAAAAAATTGGACTTCATTGTTTTGAATGAAACAAGATTTTGGTATCAAATGATCTTGCCCCCTCATTTTGATAAATCCAAGAAATATCCTCTACTATTAGATGTATATGCAGGTCCCTGTAGTCAAAAAGCAGATGCTTCCTTCAGACTGAACTGGGCCACTTACCTTGCAAGTACAGAAAACATCATAGTAGCTAGCTTTGACGGCAGAGGAAGTGGTTACCAAGGAGATAAGATCATGCATGCAATCAACAGAAGATTGGGAACACTGGAAGTTGAAGATCAAATTGAAGCAGCCAGGCAATTTGTAAAAATGGGATTTGTGGATAGCAAGCGAGTTGCAATTTGGGGCTGGTCATATGGAGGGTATGTAACCTCAATGGTCCTGGGATCGGGAAGTGGCGTGTTCAAGTGCGGAATAGCTGTGGCACCTGTGTCACGGTGGGAGTACTATGAATTCAACTTACTGACCACACAAATGTGA
Peptide sequence:
MLQDVQMPSKKLDFIVLNETRFWYQMILPPHFDKSKKYPLLLDVYAGPCSQKADASFRLNWATYLASTENIIVASFDGRGSGYQGDKIMHAINRRLGTLEVEDQIEAARQFVKMGFVDSKRVAIWGWSYGGYVTSMVLGSGSGVFKCGIAVAPVSRWEYYEFNLLTTQM
IRESfinder result
IRESfinder score
0.504911258
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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