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mmu_circDock10_590
Basic information of circular RNA
PanCircBase ID:
mmu_circDock10_590
mm39_Chr. Position ID:
chr1|80493205|80506048|-
Gene name:
Dock10
Transcript:
ENSMUST00000077946.12
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
5
Exon/intron indexes:
51,50,49,48,47
Exon/intron offsets:
0,8227,8481,10735,12744
Genomic length:
12843
Exon/intron sizes in nt:
147,114,123,107,99
Flanking exon/introns:
chr1:80490354-80493205|chr1:80506048-80506365
Splice length:
590
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTTACTGGAAAATGGAAAAGATTTGCACACCACCCCTGCTTCCAGAAGACACCCAACCCTGTGATAGCAACTTATTACTAACAACTCCAGGCGGAGGAAGCATGTTCTCTATGGGATGGCCAGCCTTTCTGAGCATCACCCCAAACATTAAAGAAGAAGGAGCAATGAAAGAGGATTCTGGAATGCAAGACACCCCGTACAATGAGAACATCCTAGTGGAACAGCTGTATATGTGTGTGGAGTTCCTTTGGAAGTCTGAACGATACGAACTCATCGCTGATGTCAATAAGCCCATCATCGCTGTCTTTGAAAAGCAACGAGACTTCAAAAAATTATCAGATCTCTATTATGACATCCACCGGTCCTATCTGAAAGTGGCAGAGGTGGTGAATTCGGAGAAGCGATTGTTTGGTCGTTACTATAGAGTGGCGTTTTATGGGCAGGGATTCTTTGAGGAGGAGGAAGGTAAAGAGTATATCTACAAAGAGCCTAAGCTGACAGGGCTCTCGGAGATCTCCCAAAGGCTTCTCAAGCTCTATGCAGACAAATTTGGAGCAGACAATGTGAAAATAATTCAAGATTCCAACAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CTCGGAGATCTCCCAAAGGC
59.89
CAGGGTTGGGTGTCTTCTGG
60.25
146
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CTCGGAGATCTCCCAAAGGC
59.89
TGCTATCACAGGGTTGGGTG
59.67
154
PCR template sequence:
TAAGCTGACAGGGCTCTCGGAGATCTCCCAAAGGCTTCTCAAGCTCTATGCAGACAAATTTGGAGCAGACAATGTGAAAATAATTCAAGATTCCAACAAGGTTACTGGAAAATGGAAAAGATTTGCACACCACCCCTGCTTCCAGAAGACACCCAACCCTGTGATAGCAACTTATTACTAACAACTCCAGGCGGAGGAAG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
7
Interacting miRNAs (#binding sites)
mmu-miR-19b-3p(1), mmu-miR-19a-3p(1), mmu-miR-23a-3p(1), mmu-miR-339-5p(1), mmu-miR-431-5p(1), mmu-miR-207(1), mmu-miR-296-3p(1)
siRNAs for circular RNA silencing
Junction sequence
AGATTCCAACAAGGTTACTGGAAAAT
siRNA sets
AGTAACCTTGTTGGAATCT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCDOCK10_590_ORF_1
0.8258
0.228618459
0.876800857
660
ORF sequence:
ATGGAAAAGATTTGCACACCACCCCTGCTTCCAGAAGACACCCAACCCTGTGATAGCAACTTATTACTAACAACTCCAGGCGGAGGAAGCATGTTCTCTATGGGATGGCCAGCCTTTCTGAGCATCACCCCAAACATTAAAGAAGAAGGAGCAATGAAAGAGGATTCTGGAATGCAAGACACCCCGTACAATGAGAACATCCTAGTGGAACAGCTGTATATGTGTGTGGAGTTCCTTTGGAAGTCTGAACGATACGAACTCATCGCTGATGTCAATAAGCCCATCATCGCTGTCTTTGAAAAGCAACGAGACTTCAAAAAATTATCAGATCTCTATTATGACATCCACCGGTCCTATCTGAAAGTGGCAGAGGTGGTGAATTCGGAGAAGCGATTGTTTGGTCGTTACTATAGAGTGGCGTTTTATGGGCAGGGATTCTTTGAGGAGGAGGAAGGTAAAGAGTATATCTACAAAGAGCCTAAGCTGACAGGGCTCTCGGAGATCTCCCAAAGGCTTCTCAAGCTCTATGCAGACAAATTTGGAGCAGACAATGTGAAAATAATTCAAGATTCCAACAAGGTTACTGGAAAATGGAAAAGATTTGCACACCACCCCTGCTTCCAGAAGACACCCAACCCTGTGATAGCAACTTATTACTAA
Peptide sequence:
MEKICTPPLLPEDTQPCDSNLLLTTPGGGSMFSMGWPAFLSITPNIKEEGAMKEDSGMQDTPYNENILVEQLYMCVEFLWKSERYELIADVNKPIIAVFEKQRDFKKLSDLYYDIHRSYLKVAEVVNSEKRLFGRYYRVAFYGQGFFEEEEGKEYIYKEPKLTGLSEISQRLLKLYADKFGADNVKIIQDSNKVTGKWKRFAHHPCFQKTPNPVIATYY
IRESfinder result
IRESfinder score
0.513850968
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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