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mmu_circDock10_237
Basic information of circular RNA
PanCircBase ID:
mmu_circDock10_237
mm39_Chr. Position ID:
chr1|80501432|80501809|-
Gene name:
Dock10
Transcript:
ENSMUST00000077946.12
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
2
Exon/intron indexes:
50,49
Exon/intron offsets:
0,254
Genomic length:
377
Exon/intron sizes in nt:
1,14,123
Flanking exon/introns:
chr1:80493352-80501432|chr1:80501809-80503940
Splice length:
237
Other database IDs:
circAtlas: mmu-Dock10_0054
CIRCpedia: HSA_CIRCpedia_93780
Human Ortholog IDs:
Mature spliced Sequence:
AACATCCTAGTGGAACAGCTGTATATGTGTGTGGAGTTCCTTTGGAAGTCTGAACGATACGAACTCATCGCTGATGTCAATAAGCCCATCATCGCTGTCTTTGAAAAGCAACGAGACTTCAAAAAATTATCAGATCTCTATTATGACATCCACCGGTCCTATCTGAAAGTGGCAGAGGTGGTGAATTCGGAGAAGCGATTGTTTGGTCGTTACTATAGAGTGGCGTTTTATGGGCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GCAGAGGTGGTGAATTCGGA
60.04
AGACAGCGATGATGGGCTTA
58.88
166
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GAAAGTGGCAGAGGTGGTGA
59.89
AGACAGCGATGATGGGCTTA
58.88
173
PCR template sequence:
CTATTATGACATCCACCGGTCCTATCTGAAAGTGGCAGAGGTGGTGAATTCGGAGAAGCGATTGTTTGGTCGTTACTATAGAGTGGCGTTTTATGGGCAGAACATCCTAGTGGAACAGCTGTATATGTGTGTGGAGTTCCTTTGGAAGTCTGAACGATACGAACTCATCGCTGATGTCAATAAGCCCATCATCGCTGTCT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
1
Interacting miRNAs (#binding sites)
mmu-miR-207(1)
siRNAs for circular RNA silencing
Junction sequence
GTTTTATGGGCAGAACATCCTAGTGG
siRNA sets
CCACTAGGATGTTCTGCCC, CACTAGGATGTTCTGCCCA, GATGTTCTGCCCATAAAAC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCDOCK10_237_ORF_1
0.9807
0.363026512
0.786027647
450
ORF sequence:
ATGTGTGTGGAGTTCCTTTGGAAGTCTGAACGATACGAACTCATCGCTGATGTCAATAAGCCCATCATCGCTGTCTTTGAAAAGCAACGAGACTTCAAAAAATTATCAGATCTCTATTATGACATCCACCGGTCCTATCTGAAAGTGGCAGAGGTGGTGAATTCGGAGAAGCGATTGTTTGGTCGTTACTATAGAGTGGCGTTTTATGGGCAGAACATCCTAGTGGAACAGCTGTATATGTGTGTGGAGTTCCTTTGGAAGTCTGAACGATACGAACTCATCGCTGATGTCAATAAGCCCATCATCGCTGTCTTTGAAAAGCAACGAGACTTCAAAAAATTATCAGATCTCTATTATGACATCCACCGGTCCTATCTGAAAGTGGCAGAGGTGGTGAATTCGGAGAAGCGATTGTTTGGTCGTTACTATAGAGTGGCGTTTTATGGGCAG
Peptide sequence:
MCVEFLWKSERYELIADVNKPIIAVFEKQRDFKKLSDLYYDIHRSYLKVAEVVNSEKRLFGRYYRVAFYGQNILVEQLYMCVEFLWKSERYELIADVNKPIIAVFEKQRDFKKLSDLYYDIHRSYLKVAEVVNSEKRLFGRYYRVAFYGQ
IRESfinder result
IRESfinder score
0.40144555
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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