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mmu_circDnajc7_967
Basic information of circular RNA
PanCircBase ID:
mmu_circDnajc7_967
mm39_Chr. Position ID:
chr11|100474419|100483799|-
Gene name:
Dnajc7
Transcript:
ENSMUST00000239499.2
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
8
Exon/intron indexes:
13,12,11,10,9,8,7,6
Exon/intron offsets:
0,617,958,1388,6304,7293,7818,9261
Genomic length:
9380
Exon/intron sizes in nt:
63,15,31,47,74,92,16,50,00,000
Flanking exon/introns:
chr11:100473962-100474419|chr11:100483799-100486991
Splice length:
967
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTTGTTTTCTGCATGGACCGTGCCCTAGAATTTGCCCCTGCCTGCCATCGATTCAAAATTCTCAAAGCAGAATGTTTAGCAATGCTTGGTCGATACCCAGAAGCACAGTTTGTGGCCAGTGACATTTTACGAATGGATTCCACCAATGCTGATGCTCTGTATGTCCGGGGTCTTTGCCTTTATTACGAAGATTGTATTGAGAAGGCAGTGCAGTTTTTTGTACAGGCTCTCAGGATGGCTCCTGACCACGAGAAGGCTTGTGTCGCTTGTAGAAATGCCAAAGCCCTTAAAGCCAAGAAGGAAGATGGGAATAAAGCCTTTAAGGAAGGAAATTACAAGCTAGCATATGAACTGTACACAGAAGCCTTGGGGATAGATCCCAACAACATAAAAACAAATGCTAAACTCTACTGTAATCGGGGTACGGTTAATTCCAAGCTTAGGCAACTGGAAGATGCCATAGAAGACTGTACAAATGCGGTGAAGCTCGATGACACTTACATCAAAGCCTACCTGAGAAGAGCTCAGTGTTACATGGACACAGAGCAGTTTGAAGAAGCCGTGCGGGACTATGAAAAAGTGTATCAGACGGAGAAAACAAAAGAACACAAACAGCTCCTTAAGAATGCACAGCTGGAACTGAAGAAGAGCAAGAGGAAAGATTACTACAAGATCCTGGGAGTGGACAAGAATGCCTCTGAGGACGAGATCAAGAAAGCTTACCGGAAACGGGCCTTGATGCACCATCCAGATCGGCACAGTGGGGCCAGTGCCGAAGTTCAGAAGGAGGAGGAGAAGAAGTTTAAGGAAGTGGGAGAGGCCTTTACCATCCTCTCTGATCCCAAGAAAAAGACTCGTTATGACAGTGGACAGGACTTGGATGAGGAGGGCATGAATATGGGCGATTTTGATGCAAACAACATCTTCAAGGCATTCTTCGGTGGTCCTGGGGGCTTCAGCTTTGAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CAGGACTTGGATGAGGAGGG
59.16
AGGCAGGGGCAAATTCTAGG
59.74
140
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AGGAGGGCATGAATATGGGC
59.59
TTGAATCGATGGCAGGCAGG
60.75
140
PCR template sequence:
GGACAGGACTTGGATGAGGAGGGCATGAATATGGGCGATTTTGATGCAAACAACATCTTCAAGGCATTCTTCGGTGGTCCTGGGGGCTTCAGCTTTGAAGGTTGTTTTCTGCATGGACCGTGCCCTAGAATTTGCCCCTGCCTGCCATCGATTCAAAATTCTCAAAGCAGAATGTTTAGCAATGCTTGGTCGATACCCAG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
6
Interacting miRNAs (#binding sites)
mmu-miR-193b-3p(2), mmu-miR-320-3p(1), mmu-miR-411-3p(1), mmu-miR-383-5p(1), mmu-miR-207(1), mmu-miR-532-5p(1)
siRNAs for circular RNA silencing
Junction sequence
TTCAGCTTTGAAGGTTGTTTTCTGCA
siRNA sets
AAAACAACCTTCAAAGCTG, AGAAAACAACCTTCAAAGC, AAACAACCTTCAAAGCTGA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCDNAJC7_967_ORF_1
1.009
0.288808149
0.99609218
1077
ORF sequence:
ATGGACCGTGCCCTAGAATTTGCCCCTGCCTGCCATCGATTCAAAATTCTCAAAGCAGAATGTTTAGCAATGCTTGGTCGATACCCAGAAGCACAGTTTGTGGCCAGTGACATTTTACGAATGGATTCCACCAATGCTGATGCTCTGTATGTCCGGGGTCTTTGCCTTTATTACGAAGATTGTATTGAGAAGGCAGTGCAGTTTTTTGTACAGGCTCTCAGGATGGCTCCTGACCACGAGAAGGCTTGTGTCGCTTGTAGAAATGCCAAAGCCCTTAAAGCCAAGAAGGAAGATGGGAATAAAGCCTTTAAGGAAGGAAATTACAAGCTAGCATATGAACTGTACACAGAAGCCTTGGGGATAGATCCCAACAACATAAAAACAAATGCTAAACTCTACTGTAATCGGGGTACGGTTAATTCCAAGCTTAGGCAACTGGAAGATGCCATAGAAGACTGTACAAATGCGGTGAAGCTCGATGACACTTACATCAAAGCCTACCTGAGAAGAGCTCAGTGTTACATGGACACAGAGCAGTTTGAAGAAGCCGTGCGGGACTATGAAAAAGTGTATCAGACGGAGAAAACAAAAGAACACAAACAGCTCCTTAAGAATGCACAGCTGGAACTGAAGAAGAGCAAGAGGAAAGATTACTACAAGATCCTGGGAGTGGACAAGAATGCCTCTGAGGACGAGATCAAGAAAGCTTACCGGAAACGGGCCTTGATGCACCATCCAGATCGGCACAGTGGGGCCAGTGCCGAAGTTCAGAAGGAGGAGGAGAAGAAGTTTAAGGAAGTGGGAGAGGCCTTTACCATCCTCTCTGATCCCAAGAAAAAGACTCGTTATGACAGTGGACAGGACTTGGATGAGGAGGGCATGAATATGGGCGATTTTGATGCAAACAACATCTTCAAGGCATTCTTCGGTGGTCCTGGGGGCTTCAGCTTTGAAGGTTGTTTTCTGCATGGACCGTGCCCTAGAATTTGCCCCTGCCTGCCATCGATTCAAAATTCTCAAAGCAGAATGTTTAGCAATGCTTGGTCGATACCCAGAAGCACAGTTTGTGGCCAGTGA
Peptide sequence:
MDRALEFAPACHRFKILKAECLAMLGRYPEAQFVASDILRMDSTNADALYVRGLCLYYEDCIEKAVQFFVQALRMAPDHEKACVACRNAKALKAKKEDGNKAFKEGNYKLAYELYTEALGIDPNNIKTNAKLYCNRGTVNSKLRQLEDAIEDCTNAVKLDDTYIKAYLRRAQCYMDTEQFEEAVRDYEKVYQTEKTKEHKQLLKNAQLELKKSKRKDYYKILGVDKNASEDEIKKAYRKRALMHHPDRHSGASAEVQKEEEKKFKEVGEAFTILSDPKKKTRYDSGQDLDEEGMNMGDFDANNIFKAFFGGPGGFSFEGCFLHGPCPRICPCLPSIQNSQSRMFSNAWSIPRSTVCGQ
IRESfinder result
IRESfinder score
0.443751971
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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