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mmu_circDnah7a_1058
Basic information of circular RNA
PanCircBase ID:
mmu_circDnah7a_1058
mm39_Chr. Position ID:
chr1|53656883|53662726|-
Gene name:
Dnah7a
Transcript:
ENSMUST00000094964.7
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
19,18,17
Exon/intron offsets:
0,2918,5618
Genomic length:
5843
Exon/intron sizes in nt:
68,61,47,225
Flanking exon/introns:
chr1:53645143-53656883|chr1:53662726-53666192
Splice length:
1058
Other database IDs:
CIRCpedia: MMU_CIRCpedia_22684, circAtlas: mmu-Dnah7a_0013
Human Ortholog IDs:
Mature spliced Sequence:
GCTCATGTTCAAAAATTGGAGACAACTGACATGTTGGAGCTGGGACAGAGACTAGTGGATTCTAAAATCTGCCTGGCCTTCCTTATCGAGTGCGTCAACTTTTCTCCTGCAGACATCAGACTAAATAAGAGTGTTTTCCAGTGGTATGGCAGAATGGGGGAGATTTTTGACGAGCACAGGAAAATCATTAAAGAGAAAACAGAACTATATCAAGAATTCCTTAAGTTTCGGTGTGAGCGGTTTGTGGAGGAATTGGAGAGTTATGCTAAACAAGTAGAAGAATTTCATGCATTTGGAGACCTGTTGGATGTGCAGAGGTACTTGCAAAAGGCCCAGGTGCTGAATGGCAAGTTGGATGCAGCAGCGGACAAGATTGATCAGTTCAATGCAGAAGAGGAGGCGTTTGGCTGGGTCCCATCAGCATATCCTCAACGTAAAAAGATCCAGGACGCTTTGAACCCTTATCTCCATCTCTATGAAACTGCTGTGGAGTTTAGTACCAAACACAGAGGGTGGACTGAAGGACCATACCACAAGGTGAACCCAGATCAAGTAGAAGCAGATGTTGGGAATTACTGGAGAGGGCTGTATAAGCTAGAGAAGGTCTTCCATGATTCTCCAAATGCGCTGGCAATGACCAAGAAAGTGCACTCAATGGTTGAGGAGTTCAAGCAGTACATCCCTCTCATCCAAGTATTCTGTAATCCTGGTCTGCGCCCCAGGCACTGGGAGGCCATGTCTACCATCGTTGGCTACCCTCTGCAGCCCTCGGATGACTCTACAGTCTTCTCTTTCATAGACATGAACCTGGAGCCATTTTTAGACAGATTTGGAAGCATCAGTGAAGCAGCTAGCAAAGAATATTCTCTTGAAAAGGCCATGGATAAGATGATGACTGAATGGGACTCCATGGAGTTTGTCATTCTTCCCTACAGAGAAAGTGGGACATACATTTTGTCCTCAGTTGATGACATTCAGATGTTGTTGGATGACCACATTATCAAAACACAAACTATGCGAGGATCTCCTTTCATCAAGCCTTATGAAAAGCAGATGAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CACAAACTATGCGAGGATCTCC
58.87
GCACTCGATAAGGAAGGCCA
59.82
145
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CACAAACTATGCGAGGATCTCC
58.87
ACGCACTCGATAAGGAAGGC
60.18
147
PCR template sequence:
CCTCAGTTGATGACATTCAGATGTTGTTGGATGACCACATTATCAAAACACAAACTATGCGAGGATCTCCTTTCATCAAGCCTTATGAAAAGCAGATGAGGCTCATGTTCAAAAATTGGAGACAACTGACATGTTGGAGCTGGGACAGAGACTAGTGGATTCTAAAATCTGCCTGGCCTTCCTTATCGAGTGCGTCAACT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
6
Interacting miRNAs (#binding sites)
mmu-miR-7b-5p(1), mmu-miR-223-3p(1), mmu-miR-33-5p(1), mmu-miR-676-3p(1), mmu-miR-541-5p(1), mmu-miR-342-3p(1)
siRNAs for circular RNA silencing
Junction sequence
AAAAGCAGATGAGGCTCATGTTCAAA
siRNA sets
TTTGAACATGAGCCTCATC, GAACATGAGCCTCATCTGC, TTGAACATGAGCCTCATCT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCDNAH7A_1058_ORF_1
1.065
0.214178951
0.995408418
1083
ORF sequence:
ATGTTGGAGCTGGGACAGAGACTAGTGGATTCTAAAATCTGCCTGGCCTTCCTTATCGAGTGCGTCAACTTTTCTCCTGCAGACATCAGACTAAATAAGAGTGTTTTCCAGTGGTATGGCAGAATGGGGGAGATTTTTGACGAGCACAGGAAAATCATTAAAGAGAAAACAGAACTATATCAAGAATTCCTTAAGTTTCGGTGTGAGCGGTTTGTGGAGGAATTGGAGAGTTATGCTAAACAAGTAGAAGAATTTCATGCATTTGGAGACCTGTTGGATGTGCAGAGGTACTTGCAAAAGGCCCAGGTGCTGAATGGCAAGTTGGATGCAGCAGCGGACAAGATTGATCAGTTCAATGCAGAAGAGGAGGCGTTTGGCTGGGTCCCATCAGCATATCCTCAACGTAAAAAGATCCAGGACGCTTTGAACCCTTATCTCCATCTCTATGAAACTGCTGTGGAGTTTAGTACCAAACACAGAGGGTGGACTGAAGGACCATACCACAAGGTGAACCCAGATCAAGTAGAAGCAGATGTTGGGAATTACTGGAGAGGGCTGTATAAGCTAGAGAAGGTCTTCCATGATTCTCCAAATGCGCTGGCAATGACCAAGAAAGTGCACTCAATGGTTGAGGAGTTCAAGCAGTACATCCCTCTCATCCAAGTATTCTGTAATCCTGGTCTGCGCCCCAGGCACTGGGAGGCCATGTCTACCATCGTTGGCTACCCTCTGCAGCCCTCGGATGACTCTACAGTCTTCTCTTTCATAGACATGAACCTGGAGCCATTTTTAGACAGATTTGGAAGCATCAGTGAAGCAGCTAGCAAAGAATATTCTCTTGAAAAGGCCATGGATAAGATGATGACTGAATGGGACTCCATGGAGTTTGTCATTCTTCCCTACAGAGAAAGTGGGACATACATTTTGTCCTCAGTTGATGACATTCAGATGTTGTTGGATGACCACATTATCAAAACACAAACTATGCGAGGATCTCCTTTCATCAAGCCTTATGAAAAGCAGATGAGGCTCATGTTCAAAAATTGGAGACAACTGACATGTTGGAGCTGGGACAGAGACTAG
Peptide sequence:
MLELGQRLVDSKICLAFLIECVNFSPADIRLNKSVFQWYGRMGEIFDEHRKIIKEKTELYQEFLKFRCERFVEELESYAKQVEEFHAFGDLLDVQRYLQKAQVLNGKLDAAADKIDQFNAEEEAFGWVPSAYPQRKKIQDALNPYLHLYETAVEFSTKHRGWTEGPYHKVNPDQVEADVGNYWRGLYKLEKVFHDSPNALAMTKKVHSMVEEFKQYIPLIQVFCNPGLRPRHWEAMSTIVGYPLQPSDDSTVFSFIDMNLEPFLDRFGSISEAASKEYSLEKAMDKMMTEWDSMEFVILPYRESGTYILSSVDDIQMLLDDHIIKTQTMRGSPFIKPYEKQMRLMFKNWRQLTCWSWDRD
IRESfinder result
IRESfinder score
0.500124741
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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