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mmu_circDnah10_498
Basic information of circular RNA
PanCircBase ID:
mmu_circDnah10_498
mm39_Chr. Position ID:
chr5|124823606|124827950|+
Gene name:
Dnah10
Transcript:
ENSMUST00000141137.7
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
11,12,13
Exon/intron offsets:
0,1848,4218
Genomic length:
4344
Exon/intron sizes in nt:
19,51,77,126
Flanking exon/introns:
chr5:124820793-124823606|chr5:124827950-124830231
Splice length:
498
Other database IDs:
CIRCpedia: MMU_CIRCpedia_229462
Human Ortholog IDs:
Mature spliced Sequence:
GTCTTGGAAGAATTTTACAACATATTTGGCCCAGAGCTCAAGGCTGTGACAGGGGACCCCAAGAGAATCGACGATGTCCTTGGCCGGGTAGACAGCTTGGTGGCTCCTATGGAAAGCCTGAGCTTTGATCCATTCAGCATCAGATCCTCCCCATACTGGAAATACGTGATGGAGGACTTCAAGCTTGAAGTTCTGATCGATATCGTTAATAAGATCTTTGTGCAGAACCAGGACAACCCGCCGCTATACAAGAACCACCCTCCCGTGGCGGGCGCGATATGCTGGGAGCGATCCCTGTTCTACAGGATCAAGCACACCATCCTGCGCTTCATGGAGGTGGAGGAACTCCTGGACAGCGAGAGGGGCCAGCAGGTGAAGCAGAGATACCTGGAGGTGGGGAGGAAGATGAAGGAGTATGAAGACGGGAAGTATGAGCATTGGAAGGAGACCACAGAGCAGAGCCTCCCAACCCTCATGAAGAAGAGCCTGCTCACCAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GGAAGGAGACCACAGAGCAG
59.75
GGCCAAGGACATCGTCGATT
60.46
144
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TGGAAGGAGACCACAGAGCA
60.47
GGCCAAGGACATCGTCGATT
60.46
145
PCR template sequence:
GAGGAAGATGAAGGAGTATGAAGACGGGAAGTATGAGCATTGGAAGGAGACCACAGAGCAGAGCCTCCCAACCCTCATGAAGAAGAGCCTGCTCACCAAGGTCTTGGAAGAATTTTACAACATATTTGGCCCAGAGCTCAAGGCTGTGACAGGGGACCCCAAGAGAATCGACGATGTCCTTGGCCGGGTAGACAGCTTGG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
6
Interacting miRNAs (#binding sites)
mmu-miR-199a-5p(1), mmu-miR-326-3p(1), mmu-miR-339-5p(1), mmu-miR-218-5p(1), mmu-miR-673-5p(1), mmu-miR-296-3p(1)
siRNAs for circular RNA silencing
Junction sequence
CCTGCTCACCAAGGTCTTGGAAGAAT
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCDNAH10_498_ORF_1
1.2859
0.494407905
0.99614471
888
ORF sequence:
ATGGAAAGCCTGAGCTTTGATCCATTCAGCATCAGATCCTCCCCATACTGGAAATACGTGATGGAGGACTTCAAGCTTGAAGTTCTGATCGATATCGTTAATAAGATCTTTGTGCAGAACCAGGACAACCCGCCGCTATACAAGAACCACCCTCCCGTGGCGGGCGCGATATGCTGGGAGCGATCCCTGTTCTACAGGATCAAGCACACCATCCTGCGCTTCATGGAGGTGGAGGAACTCCTGGACAGCGAGAGGGGCCAGCAGGTGAAGCAGAGATACCTGGAGGTGGGGAGGAAGATGAAGGAGTATGAAGACGGGAAGTATGAGCATTGGAAGGAGACCACAGAGCAGAGCCTCCCAACCCTCATGAAGAAGAGCCTGCTCACCAAGGTCTTGGAAGAATTTTACAACATATTTGGCCCAGAGCTCAAGGCTGTGACAGGGGACCCCAAGAGAATCGACGATGTCCTTGGCCGGGTAGACAGCTTGGTGGCTCCTATGGAAAGCCTGAGCTTTGATCCATTCAGCATCAGATCCTCCCCATACTGGAAATACGTGATGGAGGACTTCAAGCTTGAAGTTCTGATCGATATCGTTAATAAGATCTTTGTGCAGAACCAGGACAACCCGCCGCTATACAAGAACCACCCTCCCGTGGCGGGCGCGATATGCTGGGAGCGATCCCTGTTCTACAGGATCAAGCACACCATCCTGCGCTTCATGGAGGTGGAGGAACTCCTGGACAGCGAGAGGGGCCAGCAGGTGAAGCAGAGATACCTGGAGGTGGGGAGGAAGATGAAGGAGTATGAAGACGGGAAGTATGAGCATTGGAAGGAGACCACAGAGCAGAGCCTCCCAACCCTCATGAAGAAGAGCCTGCTCACCAAG
Peptide sequence:
MESLSFDPFSIRSSPYWKYVMEDFKLEVLIDIVNKIFVQNQDNPPLYKNHPPVAGAICWERSLFYRIKHTILRFMEVEELLDSERGQQVKQRYLEVGRKMKEYEDGKYEHWKETTEQSLPTLMKKSLLTKVLEEFYNIFGPELKAVTGDPKRIDDVLGRVDSLVAPMESLSFDPFSIRSSPYWKYVMEDFKLEVLIDIVNKIFVQNQDNPPLYKNHPPVAGAICWERSLFYRIKHTILRFMEVEELLDSERGQQVKQRYLEVGRKMKEYEDGKYEHWKETTEQSLPTLMKKSLLTK
IRESfinder result
IRESfinder score
0.489585872
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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