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mmu_circDlg1_743
Basic information of circular RNA
PanCircBase ID:
mmu_circDlg1_743
mm39_Chr. Position ID:
chr16|31624431|31661657|+
Gene name:
Dlg1
Transcript:
ENSMUST00000115205.9
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
6
Exon/intron indexes:
13,14,15,16,17,18
Exon/intron offsets:
0,7054,23852,30576,32436,37156
Genomic length:
37226
Exon/intron sizes in nt:
121,157,103,115,177,70
Flanking exon/introns:
chr16:31615828-31624431|chr16:31661657-31666453
Splice length:
743
Other database IDs:
circAtlas: mmu-Dlg1_0029
circBase: hsa_circ_0007203, circAtlas: hsa-SCAF8_0001, CIRCpedia: HSA_CIRCpedia_45074
Human Ortholog IDs:
Mature spliced Sequence:
CTTCTTCTCAATCTGTTGACAACCATGTCAGCCCGTCCTCCTGCTTGGGCCAGACGCCAACGTCACCAGCCAGGTACTCACCCATTTCTAAAGCTGTGCTCGGAGATGACGAGATCACTAGGGAACCTAGAAAAGTTGTTCTTCATCGTGGCTCAACAGGACTTGGTTTTAACATTGTGGGAGGTGAAGATGGAGAAGGGATTTTTATCTCCTTCATCCTTGCTGGCGGACCTGCTGATCTAAGTGGAGAGCTCAGAAAAGGAGATCGCATCATATCGGTGAACAGTGTTGACCTCAGAGCTGCAAGTCACGAACAAGCAGCAGCTGCACTAAAGAACGCAGGCCAAGCCGTCACCATCGTTGCGCAATATCGACCCGAAGAGTACAGTCGTTTTGAAGCTAAAATCCATGACTTACGGGAGCAGATGATGAATAGCAGTGTCAGTTCAGGGTCAGGGTCTCTTCGAACCAGCCAGAAGCGCTCCCTCTATGTCAGAGCCCTCTTTGATTATGACAAGACTAAGGACAGCGGGCTTCCCAGTCAAGGACTGAACTTCCGCTTTGGAGACATCCTCCATGTCATCAATGCTTCTGACGACGAGTGGTGGCAAGCCAGGCAGGTCACCCCAGACGGGGAGAGTGACGAAGTCGGAGTGATTCCTAGTAAACGAAGAGTTGAGAAGAAGGAACGAGCCCGATTAAAAACGGTCAAATTCAATTCTAAAACAAGAGGAGATAAAGGG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GAAGAAGGAACGAGCCCGAT
59.82
AATGGGTGAGTACCTGGCTG
59.38
150
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GAGAAGAAGGAACGAGCCCG
60.46
AATGGGTGAGTACCTGGCTG
59.38
152
PCR template sequence:
CGAAGTCGGAGTGATTCCTAGTAAACGAAGAGTTGAGAAGAAGGAACGAGCCCGATTAAAAACGGTCAAATTCAATTCTAAAACAAGAGGAGATAAAGGGCTTCTTCTCAATCTGTTGACAACCATGTCAGCCCGTCCTCCTGCTTGGGCCAGACGCCAACGTCACCAGCCAGGTACTCACCCATTTCTAAAGCTGTGCT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
6
Interacting miRNAs (#binding sites)
mmu-miR-200a-3p(1), mmu-miR-141-3p(1), mmu-miR-375-3p(1), mmu-miR-211-5p(1), mmu-miR-138-5p(1), mmu-miR-207(1)
siRNAs for circular RNA silencing
Junction sequence
AGGAGATAAAGGGCTTCTTCTCAATC
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCDLG1_743_ORF_1
0.6749
0.219220435
0.715419676
561
ORF sequence:
ATGATGAATAGCAGTGTCAGTTCAGGGTCAGGGTCTCTTCGAACCAGCCAGAAGCGCTCCCTCTATGTCAGAGCCCTCTTTGATTATGACAAGACTAAGGACAGCGGGCTTCCCAGTCAAGGACTGAACTTCCGCTTTGGAGACATCCTCCATGTCATCAATGCTTCTGACGACGAGTGGTGGCAAGCCAGGCAGGTCACCCCAGACGGGGAGAGTGACGAAGTCGGAGTGATTCCTAGTAAACGAAGAGTTGAGAAGAAGGAACGAGCCCGATTAAAAACGGTCAAATTCAATTCTAAAACAAGAGGAGATAAAGGGCTTCTTCTCAATCTGTTGACAACCATGTCAGCCCGTCCTCCTGCTTGGGCCAGACGCCAACGTCACCAGCCAGGTACTCACCCATTTCTAAAGCTGTGCTCGGAGATGACGAGATCACTAGGGAACCTAGAAAAGTTGTTCTTCATCGTGGCTCAACAGGACTTGGTTTTAACATTGTGGGAGGTGAAGATGGAGAAGGGATTTTTATCTCCTTCATCCTTGCTGGCGGACCTGCTGATCTAA
Peptide sequence:
MMNSSVSSGSGSLRTSQKRSLYVRALFDYDKTKDSGLPSQGLNFRFGDILHVINASDDEWWQARQVTPDGESDEVGVIPSKRRVEKKERARLKTVKFNSKTRGDKGLLLNLLTTMSARPPAWARRQRHQPGTHPFLKLCSEMTRSLGNLEKLFFIVAQQDLVLTLWEVKMEKGFLSPSSLLADLLI
IRESfinder result
IRESfinder score
0.377729287
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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