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mmu_circDlg1_346
Basic information of circular RNA
PanCircBase ID:
mmu_circDlg1_346
mm39_Chr. Position ID:
chr16|31606899|31610702|+
Gene name:
Dlg1
Transcript:
ENSMUST00000115205.9
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
8,9,10
Exon/intron offsets:
0,2095,3633
Genomic length:
3803
Exon/intron sizes in nt:
5,11,25,170
Flanking exon/introns:
chr16:31600653-31606899|chr16:31610702-31612321
Splice length:
346
Other database IDs:
CIRCpedia: MMU_CIRCpedia_212638
circAtlas: hsa-SCAF8_0001
Human Ortholog IDs:
Mature spliced Sequence:
GTTAATGGCACTGATGCAGATTATGAATATGAGGAAATCACACTTGAAAGGGGAAATTCGGGTCTTGGTTTCAGCATTGCAGGAGGTACAGACAACCCACACATTGGAGATGACTCAAGTATTTTCATCACCAAAATTATCACAGGCGGAGCGGCTGCCCAGGATGGAAGATTGCGGGTAAATGACTGTATACTGAGAGTAAATGAAGCAGACGTTCGTGATGTAACCCACAGCAAAGCAGTGGAGGCATTAAAAGAAGCTGGATCTATTGTGCGATTGTATGTGAAAAGGCGGAAGCCAGCATCAGAAAAAATCATGGAAATAAAGCTCATTAAAGGTCCTAAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CGGAAGCCAGCATCAGAAAA
58.83
GGGTTGTCTGTACCTCCTGC
60.04
153
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CGGAAGCCAGCATCAGAAAAA
59.46
GGGTTGTCTGTACCTCCTGC
60.04
153
PCR template sequence:
GCATTAAAAGAAGCTGGATCTATTGTGCGATTGTATGTGAAAAGGCGGAAGCCAGCATCAGAAAAAATCATGGAAATAAAGCTCATTAAAGGTCCTAAAGGTTAATGGCACTGATGCAGATTATGAATATGAGGAAATCACACTTGAAAGGGGAAATTCGGGTCTTGGTTTCAGCATTGCAGGAGGTACAGACAACCCAC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
6
Interacting miRNAs (#binding sites)
mmu-miR-363-3p(1), mmu-miR-466a-5p(1), mmu-miR-1187(1), mmu-miR-466p-5p(1), mmu-miR-335-3p(1), mmu-miR-466b-3p(1)
siRNAs for circular RNA silencing
Junction sequence
AAAGGTCCTAAAGGTTAATGGCACTG
siRNA sets
AGTGCCATTAACCTTTAGG, ATTAACCTTTAGGACCTTT, GTGCCATTAACCTTTAGGA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
NA
NA
NA
NA
NA
ORF sequence:
NA
Peptide sequence:
NA
IRESfinder result
IRESfinder score
0.362242572
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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