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mmu_circDlg1_1226
Basic information of circular RNA
PanCircBase ID:
mmu_circDlg1_1226
mm39_Chr. Position ID:
chr16|31631485|31686740|+
Gene name:
Dlg1
Transcript:
ENSMUST00000115205.9
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
12
Exon/intron indexes:
14,15,16,17,18,19,20,21,22,23,24,25
Exon/intron offsets:
0,16798,23522,25382,30102,34968,41175,43773,44879,45224,50505,55163
Genomic length:
55255
Exon/intron sizes in nt:
157,103,115,177,70,34,42,51,102,173,110,92
Flanking exon/introns:
chr16:31624552-31631485|chr16:31686740-31690492
Splice length:
1226
Other database IDs:
circAtlas: mmu-Dlg1_0072
Human Ortholog IDs:
Mature spliced Sequence:
GGAACCTAGAAAAGTTGTTCTTCATCGTGGCTCAACAGGACTTGGTTTTAACATTGTGGGAGGTGAAGATGGAGAAGGGATTTTTATCTCCTTCATCCTTGCTGGCGGACCTGCTGATCTAAGTGGAGAGCTCAGAAAAGGAGATCGCATCATATCGGTGAACAGTGTTGACCTCAGAGCTGCAAGTCACGAACAAGCAGCAGCTGCACTAAAGAACGCAGGCCAAGCCGTCACCATCGTTGCGCAATATCGACCCGAAGAGTACAGTCGTTTTGAAGCTAAAATCCATGACTTACGGGAGCAGATGATGAATAGCAGTGTCAGTTCAGGGTCAGGGTCTCTTCGAACCAGCCAGAAGCGCTCCCTCTATGTCAGAGCCCTCTTTGATTATGACAAGACTAAGGACAGCGGGCTTCCCAGTCAAGGACTGAACTTCCGCTTTGGAGACATCCTCCATGTCATCAATGCTTCTGACGACGAGTGGTGGCAAGCCAGGCAGGTCACCCCAGACGGGGAGAGTGACGAAGTCGGAGTGATTCCTAGTAAACGAAGAGTTGAGAAGAAGGAACGAGCCCGATTAAAAACGGTCAAATTCAATTCTAAAACAAGAGGAGATAAAGGGGAGATCCCTGACGACATGGGATCAAAAGGCCTGAAGCACGTAACTTCTAATGCCAGCGATAGTGAAAGTAGTTACCGTGGTCAAGAAGAATATGTTTTATCTTATGAGCCAGTGAATCAACAAGAAGTTAATTATACCCGACCAGTCATCATATTAGGACCTATGAAAGACAGAGTAAATGATGACTTAATCTCAGAATTTCCTGACAAATTTGGATCCTGTGTCCCTCATACAACTAGACCGAAGCGTGACTATGAGGTGGATGGACGAGATTATCATTTTGTGACTTCAAGGGAACAGATGGAAAAGGATATTCAGGAGCATAAGTTCATTGAAGCCGGCCAGTATAACAACCATCTGTATGGGACGAGCGTGCAGTCCGTGCGAGCAGTGGCAGAGAAGGGCAAACATTGTATCCTTGATGTGTCTGGAAATGCCATAAAGAGGTTGCAGATTGCACAGCTTTATCCAATATCTATTTTTATTAAACCCAAATCCATGGAAAATATCATGGAAATGAACAAGCGCCTAACAGAAGAGCAGGCCAGAAAAACATTTGAGAGAGCCATGAAGCTGGAGCAGGAGTTCACTGAGCATTTCACAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AGCGCCTAACAGAAGAGCAG
60.11
TCTCCATCTTCACCTCCCACA
60.2
156
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TCATGGAAATGAACAAGCGCC
59.8
ACCAAGTCCTGTTGAGCCAC
60.18
142
PCR template sequence:
AATATCATGGAAATGAACAAGCGCCTAACAGAAGAGCAGGCCAGAAAAACATTTGAGAGAGCCATGAAGCTGGAGCAGGAGTTCACTGAGCATTTCACAGGGAACCTAGAAAAGTTGTTCTTCATCGTGGCTCAACAGGACTTGGTTTTAACATTGTGGGAGGTGAAGATGGAGAAGGGATTTTTATCTCCTTCATCCTT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
7
Interacting miRNAs (#binding sites)
mmu-miR-211-5p(2), mmu-miR-200a-3p(1), mmu-miR-141-3p(1), mmu-miR-375-3p(1), mmu-miR-203-3p(1), mmu-miR-224-5p(1), mmu-miR-207(1)
siRNAs for circular RNA silencing
Junction sequence
GAGCATTTCACAGGGAACCTAGAAAA
siRNA sets
AGGTTCCCTGTGAAATGCT, CTAGGTTCCCTGTGAAATG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCDLG1_1226_ORF_1
0.9185
0.252487146
0.993130792
1044
ORF sequence:
ATGATGAATAGCAGTGTCAGTTCAGGGTCAGGGTCTCTTCGAACCAGCCAGAAGCGCTCCCTCTATGTCAGAGCCCTCTTTGATTATGACAAGACTAAGGACAGCGGGCTTCCCAGTCAAGGACTGAACTTCCGCTTTGGAGACATCCTCCATGTCATCAATGCTTCTGACGACGAGTGGTGGCAAGCCAGGCAGGTCACCCCAGACGGGGAGAGTGACGAAGTCGGAGTGATTCCTAGTAAACGAAGAGTTGAGAAGAAGGAACGAGCCCGATTAAAAACGGTCAAATTCAATTCTAAAACAAGAGGAGATAAAGGGGAGATCCCTGACGACATGGGATCAAAAGGCCTGAAGCACGTAACTTCTAATGCCAGCGATAGTGAAAGTAGTTACCGTGGTCAAGAAGAATATGTTTTATCTTATGAGCCAGTGAATCAACAAGAAGTTAATTATACCCGACCAGTCATCATATTAGGACCTATGAAAGACAGAGTAAATGATGACTTAATCTCAGAATTTCCTGACAAATTTGGATCCTGTGTCCCTCATACAACTAGACCGAAGCGTGACTATGAGGTGGATGGACGAGATTATCATTTTGTGACTTCAAGGGAACAGATGGAAAAGGATATTCAGGAGCATAAGTTCATTGAAGCCGGCCAGTATAACAACCATCTGTATGGGACGAGCGTGCAGTCCGTGCGAGCAGTGGCAGAGAAGGGCAAACATTGTATCCTTGATGTGTCTGGAAATGCCATAAAGAGGTTGCAGATTGCACAGCTTTATCCAATATCTATTTTTATTAAACCCAAATCCATGGAAAATATCATGGAAATGAACAAGCGCCTAACAGAAGAGCAGGCCAGAAAAACATTTGAGAGAGCCATGAAGCTGGAGCAGGAGTTCACTGAGCATTTCACAGGGAACCTAGAAAAGTTGTTCTTCATCGTGGCTCAACAGGACTTGGTTTTAACATTGTGGGAGGTGAAGATGGAGAAGGGATTTTTATCTCCTTCATCCTTGCTGGCGGACCTGCTGATCTAA
Peptide sequence:
MMNSSVSSGSGSLRTSQKRSLYVRALFDYDKTKDSGLPSQGLNFRFGDILHVINASDDEWWQARQVTPDGESDEVGVIPSKRRVEKKERARLKTVKFNSKTRGDKGEIPDDMGSKGLKHVTSNASDSESSYRGQEEYVLSYEPVNQQEVNYTRPVIILGPMKDRVNDDLISEFPDKFGSCVPHTTRPKRDYEVDGRDYHFVTSREQMEKDIQEHKFIEAGQYNNHLYGTSVQSVRAVAEKGKHCILDVSGNAIKRLQIAQLYPISIFIKPKSMENIMEMNKRLTEEQARKTFERAMKLEQEFTEHFTGNLEKLFFIVAQQDLVLTLWEVKMEKGFLSPSSLLADLLI
IRESfinder result
IRESfinder score
0.49593665
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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