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mmu_circDip2a_1614
Basic information of circular RNA
PanCircBase ID:
mmu_circDip2a_1614
mm39_Chr. Position ID:
chr10|76130327|76155420|-
Gene name:
Dip2a
Transcript:
ENSMUST00000160048.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
13
Exon/intron indexes:
15,14,13,12,11,10,9,8,7,6,5,4,3
Exon/intron offsets:
0,1573,1859,3261,3759,4176,5409,7710,10522,12579,18682,23373,24973
Genomic length:
25093
Exon/intron sizes in nt:
120,94,65,103,110,124,111,92,198,120,129,228,120
Flanking exon/introns:
chr10:76128260-76130327|chr10:76155420-76163535
Splice length:
1614
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
ATGTTCACACGGAAGCCGTGCAAGCAGCTTTGGCCAAGTACAAGGAGAGGAAGATGCCCATGCCTTCAAAGAGACGCTCCGCCCTTGTGCACTCCTCCGTTGAGACCTACACGCCTCCAGACACGTCATCTGCCTCAGAAGATGAGGGCTCTTTACGGCGACCTGGGCGACTCACCTCCACTCTGCTCCAGAGCCATTCCGGCATCGAGCCCTGGCTCGACAGGGTCATTCAGGGCTCCTCCACTTCATCCTCTGCATCCTCCACCTCATCCCACCCGGGAGGGAGACCTGCTGCTGCTCCCAGTGCCTCCACTGCGCTCGCAGGCCTCACGGCCCACGCCCACATAGATCTGCACTCTGCCCCACCGGATGTCACCACTGGGCTTGTGGAGCACTCATCCTACGAGCGTCCCCAGATGGCCTCTGTGCGAGGCATTCCTCGAGGGCACGGCAGGAACGTGCTGGAGACCGCGGACGGTGTGCCTGTGAACAGCAGAGTGTCCTCCAAAATCCAGCAGCTACTGAACACCCTGAAGAGGCCAAAGCGCCCTCCTCTGAAGGAATTTTTTGTGGATGATTTTGAGGAGTTACTGGAAGTTCAGCAACCAGATCCGAACCAGCCCAAGCCTGAGGGGGATCAGATGGCCGTACTGAAGGGAGAGCCTCTGTCAGTGGGGACTAATGGGCCACTGTCTCTGTTGGCTGCACTGCAGCTCTGGGGCACAACACAGCCGAAAGCCCCCTGTCTGACTGCCTTGGACACAGCGGGGAAAGCCACCTGCACCCTGACCTATGGCAAACTCTGGAGTCGGAGTCTAAAATTAGCGTATACTCTACTTAACAAACTGACTAGTAAGAATGAGCCCCTGCTTAACCCTGGAGACAGAGTGGCACTCGTGTTTCCAAACAGTGACCCTGTCATGTTCATGGTGGCGTTCTACGGGTGTCTCCTGGCGGAGCTGGTCCCTGTCCCTATAGAAGTACCACTAACGAGAAAGGATGCAGGCAGCCAGCAGGTTGGGTTTCTCCTGGGCAGCTGTGGGGTGACCCTGGCCCTGACCACAGATGCTTGTCAGAAGGGTCTGCCCAAGGCACCGACAGGCGAGGTGGCCACTTTCAAAGGTTGGCCCCCCCTCGCCTGGCTGGTGATTGATGGGAAGCATCTGACGAGGCCTCCAAAGGACTGGTACCCGCTGGCCCAGGACACAGGGTCCAGGACTGCCTACATTGAATATAAAACCAGCAAAGAAGGCAGCACAGTAGGGGTCACCGTATCCCACTCATCCCTGCTGGCGCAGTGCCAGGCCCTGACCCAGGCGTGCGGGTACACGGAAGCTGAAACGTTAACGAATGTGCTGGACTTCAAAAGGGATGCTGGTTTGTGGCACGGAGTCTTAACAAGTGTCATGAATAGGATGCATGTCATCACCATCCCATATGCCCTCATGAAGGTCAACCCACTCTCCTGGATTCAGAAAGTGTGTTCCTATAAAGCCCGGGCTGCCCTGGTAAAGTCCCGAGATATGCACTGGTCGCTCTTGGCACAGCGAGGTCAGAGGGATGTCTGTCTCAGCTCTCTGCGCATGTTGATTGTGGCAGACGGCGCTAACCCAT
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CGAGATATGCACTGGTCGCT
59.97
AAGGCATGGGCATCTTCCTC
60.11
163
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CGAGATATGCACTGGTCGCT
59.97
GAAGGCATGGGCATCTTCCT
60.11
164
PCR template sequence:
TCCCGAGATATGCACTGGTCGCTCTTGGCACAGCGAGGTCAGAGGGATGTCTGTCTCAGCTCTCTGCGCATGTTGATTGTGGCAGACGGCGCTAACCCATATGTTCACACGGAAGCCGTGCAAGCAGCTTTGGCCAAGTACAAGGAGAGGAAGATGCCCATGCCTTCAAAGAGACGCTCCGCCCTTGTGCACTCCTCCGT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
20
Interacting miRNAs (#binding sites)
mmu-miR-152-3p(2), mmu-miR-148b-3p(2), mmu-miR-148a-3p(2), mmu-miR-3470b(2), mmu-miR-34c-5p(1), mmu-miR-223-3p(1), mmu-miR-211-5p(1), mmu-miR-204-5p(1), mmu-miR-298-5p(1), mmu-miR-339-5p(1), mmu-miR-10b-5p(1), mmu-miR-10a-5p(1), mmu-miR-351-5p(1), mmu-miR-363-3p(1), mmu-miR-29b-1-5p(1), mmu-miR-22-3p(1), mmu-miR-802-5p(1), mmu-miR-544-5p(1), mmu-miR-296-3p(1), mmu-miR-99a-5p(1)
siRNAs for circular RNA silencing
Junction sequence
GGCGCTAACCCATATGTTCACACGGA
siRNA sets
TGAACATATGGGTTAGCGC, TGTGAACATATGGGTTAGC, GAACATATGGGTTAGCGCC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCDIP2A_1614_ORF_1
1.0588
0.278235371
0.999999999
3174
ORF sequence:
ATGCCCATGCCTTCAAAGAGACGCTCCGCCCTTGTGCACTCCTCCGTTGAGACCTACACGCCTCCAGACACGTCATCTGCCTCAGAAGATGAGGGCTCTTTACGGCGACCTGGGCGACTCACCTCCACTCTGCTCCAGAGCCATTCCGGCATCGAGCCCTGGCTCGACAGGGTCATTCAGGGCTCCTCCACTTCATCCTCTGCATCCTCCACCTCATCCCACCCGGGAGGGAGACCTGCTGCTGCTCCCAGTGCCTCCACTGCGCTCGCAGGCCTCACGGCCCACGCCCACATAGATCTGCACTCTGCCCCACCGGATGTCACCACTGGGCTTGTGGAGCACTCATCCTACGAGCGTCCCCAGATGGCCTCTGTGCGAGGCATTCCTCGAGGGCACGGCAGGAACGTGCTGGAGACCGCGGACGGTGTGCCTGTGAACAGCAGAGTGTCCTCCAAAATCCAGCAGCTACTGAACACCCTGAAGAGGCCAAAGCGCCCTCCTCTGAAGGAATTTTTTGTGGATGATTTTGAGGAGTTACTGGAAGTTCAGCAACCAGATCCGAACCAGCCCAAGCCTGAGGGGGATCAGATGGCCGTACTGAAGGGAGAGCCTCTGTCAGTGGGGACTAATGGGCCACTGTCTCTGTTGGCTGCACTGCAGCTCTGGGGCACAACACAGCCGAAAGCCCCCTGTCTGACTGCCTTGGACACAGCGGGGAAAGCCACCTGCACCCTGACCTATGGCAAACTCTGGAGTCGGAGTCTAAAATTAGCGTATACTCTACTTAACAAACTGACTAGTAAGAATGAGCCCCTGCTTAACCCTGGAGACAGAGTGGCACTCGTGTTTCCAAACAGTGACCCTGTCATGTTCATGGTGGCGTTCTACGGGTGTCTCCTGGCGGAGCTGGTCCCTGTCCCTATAGAAGTACCACTAACGAGAAAGGATGCAGGCAGCCAGCAGGTTGGGTTTCTCCTGGGCAGCTGTGGGGTGACCCTGGCCCTGACCACAGATGCTTGTCAGAAGGGTCTGCCCAAGGCACCGACAGGCGAGGTGGCCACTTTCAAAGGTTGGCCCCCCCTCGCCTGGCTGGTGATTGATGGGAAGCATCTGACGAGGCCTCCAAAGGACTGGTACCCGCTGGCCCAGGACACAGGGTCCAGGACTGCCTACATTGAATATAAAACCAGCAAAGAAGGCAGCACAGTAGGGGTCACCGTATCCCACTCATCCCTGCTGGCGCAGTGCCAGGCCCTGACCCAGGCGTGCGGGTACACGGAAGCTGAAACGTTAACGAATGTGCTGGACTTCAAAAGGGATGCTGGTTTGTGGCACGGAGTCTTAACAAGTGTCATGAATAGGATGCATGTCATCACCATCCCATATGCCCTCATGAAGGTCAACCCACTCTCCTGGATTCAGAAAGTGTGTTCCTATAAAGCCCGGGCTGCCCTGGTAAAGTCCCGAGATATGCACTGGTCGCTCTTGGCACAGCGAGGTCAGAGGGATGTCTGTCTCAGCTCTCTGCGCATGTTGATTGTGGCAGACGGCGCTAACCCATATGTTCACACGGAAGCCGTGCAAGCAGCTTTGGCCAAGTACAAGGAGAGGAAGATGCCCATGCCTTCAAAGAGACGCTCCGCCCTTGTGCACTCCTCCGTTGAGACCTACACGCCTCCAGACACGTCATCTGCCTCAGAAGATGAGGGCTCTTTACGGCGACCTGGGCGACTCACCTCCACTCTGCTCCAGAGCCATTCCGGCATCGAGCCCTGGCTCGACAGGGTCATTCAGGGCTCCTCCACTTCATCCTCTGCATCCTCCACCTCATCCCACCCGGGAGGGAGACCTGCTGCTGCTCCCAGTGCCTCCACTGCGCTCGCAGGCCTCACGGCCCACGCCCACATAGATCTGCACTCTGCCCCACCGGATGTCACCACTGGGCTTGTGGAGCACTCATCCTACGAGCGTCCCCAGATGGCCTCTGTGCGAGGCATTCCTCGAGGGCACGGCAGGAACGTGCTGGAGACCGCGGACGGTGTGCCTGTGAACAGCAGAGTGTCCTCCAAAATCCAGCAGCTACTGAACACCCTGAAGAGGCCAAAGCGCCCTCCTCTGAAGGAATTTTTTGTGGATGATTTTGAGGAGTTACTGGAAGTTCAGCAACCAGATCCGAACCAGCCCAAGCCTGAGGGGGATCAGATGGCCGTACTGAAGGGAGAGCCTCTGTCAGTGGGGACTAATGGGCCACTGTCTCTGTTGGCTGCACTGCAGCTCTGGGGCACAACACAGCCGAAAGCCCCCTGTCTGACTGCCTTGGACACAGCGGGGAAAGCCACCTGCACCCTGACCTATGGCAAACTCTGGAGTCGGAGTCTAAAATTAGCGTATACTCTACTTAACAAACTGACTAGTAAGAATGAGCCCCTGCTTAACCCTGGAGACAGAGTGGCACTCGTGTTTCCAAACAGTGACCCTGTCATGTTCATGGTGGCGTTCTACGGGTGTCTCCTGGCGGAGCTGGTCCCTGTCCCTATAGAAGTACCACTAACGAGAAAGGATGCAGGCAGCCAGCAGGTTGGGTTTCTCCTGGGCAGCTGTGGGGTGACCCTGGCCCTGACCACAGATGCTTGTCAGAAGGGTCTGCCCAAGGCACCGACAGGCGAGGTGGCCACTTTCAAAGGTTGGCCCCCCCTCGCCTGGCTGGTGATTGATGGGAAGCATCTGACGAGGCCTCCAAAGGACTGGTACCCGCTGGCCCAGGACACAGGGTCCAGGACTGCCTACATTGAATATAAAACCAGCAAAGAAGGCAGCACAGTAGGGGTCACCGTATCCCACTCATCCCTGCTGGCGCAGTGCCAGGCCCTGACCCAGGCGTGCGGGTACACGGAAGCTGAAACGTTAACGAATGTGCTGGACTTCAAAAGGGATGCTGGTTTGTGGCACGGAGTCTTAACAAGTGTCATGAATAGGATGCATGTCATCACCATCCCATATGCCCTCATGAAGGTCAACCCACTCTCCTGGATTCAGAAAGTGTGTTCCTATAAAGCCCGGGCTGCCCTGGTAAAGTCCCGAGATATGCACTGGTCGCTCTTGGCACAGCGAGGTCAGAGGGATGTCTGTCTCAGCTCTCTGCGCATGTTGATTGTGGCAGACGGCGCTAACCCA
Peptide sequence:
MPMPSKRRSALVHSSVETYTPPDTSSASEDEGSLRRPGRLTSTLLQSHSGIEPWLDRVIQGSSTSSSASSTSSHPGGRPAAAPSASTALAGLTAHAHIDLHSAPPDVTTGLVEHSSYERPQMASVRGIPRGHGRNVLETADGVPVNSRVSSKIQQLLNTLKRPKRPPLKEFFVDDFEELLEVQQPDPNQPKPEGDQMAVLKGEPLSVGTNGPLSLLAALQLWGTTQPKAPCLTALDTAGKATCTLTYGKLWSRSLKLAYTLLNKLTSKNEPLLNPGDRVALVFPNSDPVMFMVAFYGCLLAELVPVPIEVPLTRKDAGSQQVGFLLGSCGVTLALTTDACQKGLPKAPTGEVATFKGWPPLAWLVIDGKHLTRPPKDWYPLAQDTGSRTAYIEYKTSKEGSTVGVTVSHSSLLAQCQALTQACGYTEAETLTNVLDFKRDAGLWHGVLTSVMNRMHVITIPYALMKVNPLSWIQKVCSYKARAALVKSRDMHWSLLAQRGQRDVCLSSLRMLIVADGANPYVHTEAVQAALAKYKERKMPMPSKRRSALVHSSVETYTPPDTSSASEDEGSLRRPGRLTSTLLQSHSGIEPWLDRVIQGSSTSSSASSTSSHPGGRPAAAPSASTALAGLTAHAHIDLHSAPPDVTTGLVEHSSYERPQMASVRGIPRGHGRNVLETADGVPVNSRVSSKIQQLLNTLKRPKRPPLKEFFVDDFEELLEVQQPDPNQPKPEGDQMAVLKGEPLSVGTNGPLSLLAALQLWGTTQPKAPCLTALDTAGKATCTLTYGKLWSRSLKLAYTLLNKLTSKNEPLLNPGDRVALVFPNSDPVMFMVAFYGCLLAELVPVPIEVPLTRKDAGSQQVGFLLGSCGVTLALTTDACQKGLPKAPTGEVATFKGWPPLAWLVIDGKHLTRPPKDWYPLAQDTGSRTAYIEYKTSKEGSTVGVTVSHSSLLAQCQALTQACGYTEAETLTNVLDFKRDAGLWHGVLTSVMNRMHVITIPYALMKVNPLSWIQKVCSYKARAALVKSRDMHWSLLAQRGQRDVCLSSLRMLIVADGANP
IRESfinder result
IRESfinder score
0.415887958
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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