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mmu_circDicer1_1951
Basic information of circular RNA
PanCircBase ID:
mmu_circDicer1_1951
mm39_Chr. Position ID:
chr12|104678439|104698246|-
Gene name:
Dicer1
Transcript:
ENSMUST00000041987.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
10
Exon/intron indexes:
11,10,9,8,7,6,5,4,3,2
Exon/intron offsets:
0,814,2593,9790,11721,16152,16947,17952,18839,19619
Genomic length:
19807
Exon/intron sizes in nt:
155,243,133,473,169,161,135,131,163,188
Flanking exon/introns:
chr12:104677016-104678439|chr12:104698246-104718006
Splice length:
1951
Other database IDs:
CIRCpedia: MMU_CIRCpedia_1324, circAtlas: mmu-Dicer1_0009
circAtlas: hsa-DICER1_0012, CIRCpedia: HSA_CIRCpedia_167973
Human Ortholog IDs:
Mature spliced Sequence:
TAAGCTGTGCTAGAACAAAATGCAATGAAAGAAACACTGGATGATTGAAAAGCCCTGCTTTGCAGCCCCTCAGCATGGCAGGCCTGCAGCTCATGACCCCTGCTTCCTCACCAATGGGTCCTTTCTTTGGACTGCCATGGCAACAAGAAGCAATTCATGATAACATTTATACGCCAAGAAAATACCAGGTTGAGCTGCTGGAAGCAGCTCTGGACCATAACACAATTGTGTGCTTGAACACTGGCTCAGGGAAGACGTTCATCGCGGTCCTGCTCACCAAAGAGCTGGCCCATCAGATCAGGGGCGACCTCAACCCGCATGCAAAAAGGACCGTGTTCCTCGTCAACTCTGCAAACCAGGTTGCTCAACAAGTGTCAGCCGTCAGAACTCACTCGGATCTCAAGGTTGGAGAGTACTCAGACCTAGAAGTAAATGCATCTTGGACAAAAGAGAGATGGAGCCAAGAGTTTACTAAGCACCAGGTTCTCATTATGACTTGCTATGTCGCCTTGACTGTTTTGAAAAATGGTTACTTATCACTGTCAGACATTAACCTTTTGGTGTTTGATGAGTGTCATCTTGCGATTCTAGACCACCCCTATCGTGAAATTATGAAGCTCTGTGAGAGTTGTCCATCATGCCCTCGAATTTTGGGACTAACTGCTTCCATTTTAAATGGGAAATGTGATCCAGAGGAATTGGAAGAGAAGATTCAGAAACTGGAGCGAATTCTCAGGAGTGACGCTGAGACGGCGACTGACTTGGTGGTCCTGGACAGATACACTTCTCAGCCTTGTGAGATTGTGGTAGACTGTGGACCGTTTACTGACAGAAGTGGGCTGTATGAGAGATTGCTGATGGAGTTAGAAGCAGCACTTGATTTTATCAATGATTGTAATGTAGCTGTACATTCAAAGGAAAGAGATTCTACTTTGATTTCCAAACAAATCCTCTCAGACTGCCGTGCAGTGTTGGTTGTTCTGGGACCCTGGTGTGCAGATAAAGTAGCTGGGATGATGGTAAGAGAACTTCAGAAGTACATCAAACATGAGCAAGAGGAGCTACACAGGAAGTTCCTATTGTTTACAGACACCTTGTTAAGGAAAATACACGCCCTGTGTGAAGAGTACTTCTCACCTGCCTCACTTGACCTGAAGTATGTAACTCCCAAAGTCATGAAACTGCTCGAGATCCTGCGCAAATACAAGCCCTATGAGCGACAGCAGTTTGAAAGTGTTGAGTGGTACAATAACAGGAATCAGGATAATTACGTGTCCTGGAGTGATTCCGAGGATGACGATGATGACGAAGAAATTGAAGAAAAAGAAAAGCCAGAGACAAATTTTCCTTCTCCGTTTACCAATATTTTATGTGGGATTATTTTTGTGGAAAGAAGATACACAGCAGTTGTCCTAAACAGATTGATAAAGGAAGCTGGCAAACAAGATCCAGAGCTGGCTTATATCAGCAGCAACTTTATAACTGGACACGGCATTGGAAAGAACCAGCCTCGCAGCAAACAGATGGAGGCGGAGTTCAGAAAGCAGGAGGAGGTACTTAGGAAATTTCGAGCTCATGAGACCAACCTGCTCATTGCAACAAGTGTTGTAGAAGAGGGTGTGGATATTCCCAAGTGCAATTTGGTGGTTCGTTTTGATTTGCCTACAGAGTATCGATCATATGTCCAGTCTAAAGGACGGGCACGAGCGCCGATCTCTAATTACGTAATGTTAGCAGATACAGACAAGATAAAGAGCTTTGAAGAAGACCTCAAGACCTACAAAGCTATTGAAAAGATCTTAAGGAACAAGTGTTCGAAGTCAGCGGATGGTGCTGAGGCGGACGTCCATGCTGGTGTAGATGATGAGGATGCTTTCCCGCCATATGTGCTGAGGCCTGATGATGGGGGTCCTCGAGTCACAATCAACACTGCCATTGGACACATCAACAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GAGGATGCTTTCCCGCCATA
59.89
GCAGGGCTTTTCAATCATCCA
59.17
144
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GAGGATGCTTTCCCGCCATA
59.89
AGCAGGGCTTTTCAATCATCC
58.89
145
PCR template sequence:
CTGGTGTAGATGATGAGGATGCTTTCCCGCCATATGTGCTGAGGCCTGATGATGGGGGTCCTCGAGTCACAATCAACACTGCCATTGGACACATCAACAGTAAGCTGTGCTAGAACAAAATGCAATGAAAGAAACACTGGATGATTGAAAAGCCCTGCTTTGCAGCCCCTCAGCATGGCAGGCCTGCAGCTCATGACCCC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
22
Interacting miRNAs (#binding sites)
mmu-miR-34b-5p(2), mmu-miR-200a-3p(1), mmu-miR-141-3p(1), mmu-miR-199a-5p(1), mmu-miR-34c-5p(1), mmu-miR-92a-3p(1), mmu-miR-29b-3p(1), mmu-miR-29a-3p(1), mmu-miR-29c-3p(1), mmu-miR-216a-5p(1), mmu-miR-132-3p(1), mmu-miR-709(1), mmu-miR-25-3p(1), mmu-miR-136-5p(1), mmu-miR-433-3p(1), mmu-miR-351-5p(1), mmu-miR-363-3p(1), mmu-miR-129-5p(1), mmu-miR-5101(1), mmu-miR-188-3p(1), mmu-miR-670-5p(1), mmu-miR-542-5p(1)
siRNAs for circular RNA silencing
Junction sequence
GACACATCAACAGTAAGCTGTGCTAG
siRNA sets
AGCTTACTGTTGATGTGTC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCDICER1_1951_ORF_1
0.9866
0.195200845
0.999982219
1890
ORF sequence:
ATGGCAGGCCTGCAGCTCATGACCCCTGCTTCCTCACCAATGGGTCCTTTCTTTGGACTGCCATGGCAACAAGAAGCAATTCATGATAACATTTATACGCCAAGAAAATACCAGGTTGAGCTGCTGGAAGCAGCTCTGGACCATAACACAATTGTGTGCTTGAACACTGGCTCAGGGAAGACGTTCATCGCGGTCCTGCTCACCAAAGAGCTGGCCCATCAGATCAGGGGCGACCTCAACCCGCATGCAAAAAGGACCGTGTTCCTCGTCAACTCTGCAAACCAGGTTGCTCAACAAGTGTCAGCCGTCAGAACTCACTCGGATCTCAAGGTTGGAGAGTACTCAGACCTAGAAGTAAATGCATCTTGGACAAAAGAGAGATGGAGCCAAGAGTTTACTAAGCACCAGGTTCTCATTATGACTTGCTATGTCGCCTTGACTGTTTTGAAAAATGGTTACTTATCACTGTCAGACATTAACCTTTTGGTGTTTGATGAGTGTCATCTTGCGATTCTAGACCACCCCTATCGTGAAATTATGAAGCTCTGTGAGAGTTGTCCATCATGCCCTCGAATTTTGGGACTAACTGCTTCCATTTTAAATGGGAAATGTGATCCAGAGGAATTGGAAGAGAAGATTCAGAAACTGGAGCGAATTCTCAGGAGTGACGCTGAGACGGCGACTGACTTGGTGGTCCTGGACAGATACACTTCTCAGCCTTGTGAGATTGTGGTAGACTGTGGACCGTTTACTGACAGAAGTGGGCTGTATGAGAGATTGCTGATGGAGTTAGAAGCAGCACTTGATTTTATCAATGATTGTAATGTAGCTGTACATTCAAAGGAAAGAGATTCTACTTTGATTTCCAAACAAATCCTCTCAGACTGCCGTGCAGTGTTGGTTGTTCTGGGACCCTGGTGTGCAGATAAAGTAGCTGGGATGATGGTAAGAGAACTTCAGAAGTACATCAAACATGAGCAAGAGGAGCTACACAGGAAGTTCCTATTGTTTACAGACACCTTGTTAAGGAAAATACACGCCCTGTGTGAAGAGTACTTCTCACCTGCCTCACTTGACCTGAAGTATGTAACTCCCAAAGTCATGAAACTGCTCGAGATCCTGCGCAAATACAAGCCCTATGAGCGACAGCAGTTTGAAAGTGTTGAGTGGTACAATAACAGGAATCAGGATAATTACGTGTCCTGGAGTGATTCCGAGGATGACGATGATGACGAAGAAATTGAAGAAAAAGAAAAGCCAGAGACAAATTTTCCTTCTCCGTTTACCAATATTTTATGTGGGATTATTTTTGTGGAAAGAAGATACACAGCAGTTGTCCTAAACAGATTGATAAAGGAAGCTGGCAAACAAGATCCAGAGCTGGCTTATATCAGCAGCAACTTTATAACTGGACACGGCATTGGAAAGAACCAGCCTCGCAGCAAACAGATGGAGGCGGAGTTCAGAAAGCAGGAGGAGGTACTTAGGAAATTTCGAGCTCATGAGACCAACCTGCTCATTGCAACAAGTGTTGTAGAAGAGGGTGTGGATATTCCCAAGTGCAATTTGGTGGTTCGTTTTGATTTGCCTACAGAGTATCGATCATATGTCCAGTCTAAAGGACGGGCACGAGCGCCGATCTCTAATTACGTAATGTTAGCAGATACAGACAAGATAAAGAGCTTTGAAGAAGACCTCAAGACCTACAAAGCTATTGAAAAGATCTTAAGGAACAAGTGTTCGAAGTCAGCGGATGGTGCTGAGGCGGACGTCCATGCTGGTGTAGATGATGAGGATGCTTTCCCGCCATATGTGCTGAGGCCTGATGATGGGGGTCCTCGAGTCACAATCAACACTGCCATTGGACACATCAACAGTAAGCTGTGCTAG
Peptide sequence:
MAGLQLMTPASSPMGPFFGLPWQQEAIHDNIYTPRKYQVELLEAALDHNTIVCLNTGSGKTFIAVLLTKELAHQIRGDLNPHAKRTVFLVNSANQVAQQVSAVRTHSDLKVGEYSDLEVNASWTKERWSQEFTKHQVLIMTCYVALTVLKNGYLSLSDINLLVFDECHLAILDHPYREIMKLCESCPSCPRILGLTASILNGKCDPEELEEKIQKLERILRSDAETATDLVVLDRYTSQPCEIVVDCGPFTDRSGLYERLLMELEAALDFINDCNVAVHSKERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKYIKHEQEELHRKFLLFTDTLLRKIHALCEEYFSPASLDLKYVTPKVMKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDDDDEEIEEKEKPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRSKQMEAEFRKQEEVLRKFRAHETNLLIATSVVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYVMLADTDKIKSFEEDLKTYKAIEKILRNKCSKSADGAEADVHAGVDDEDAFPPYVLRPDDGGPRVTINTAIGHINSKLC
IRESfinder result
IRESfinder score
0.532834858
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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