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mmu_circDhx8_1097
Basic information of circular RNA
PanCircBase ID:
mmu_circDhx8_1097
mm39_Chr. Position ID:
chr11|101640710|101645698|+
Gene name:
Dhx8
Transcript:
ENSMUST00000039152.14
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
6
Exon/intron indexes:
12,13,14,15,16,17
Exon/intron offsets:
0,993,2126,2400,3263,4847
Genomic length:
4988
Exon/intron sizes in nt:
182,195,186,211,182,141
Flanking exon/introns:
chr11:101636079-101640710|chr11:101645698-101648418
Splice length:
1097
Other database IDs:
circBase: hsa_circ_0043988, circAtlas: hsa-DHX8_0019, CIRCpedia: HSA_CIRCpedia_20933
Human Ortholog IDs:
Mature spliced Sequence:
CGGAAGGCAGGCAGATCGCTGCCAACATGAGGGGGATTGGGATGATGCCCAACGACATTCCTGAGTGGAAGAAGCATGCCTTTGGGGGCAACAAAGCTTCTTATGGGAAGAAGACCCAGATGTCAATCCTTGAGCAGAGAGAGAGCCTGCCCATCTACAAACTGAAGGAGCAGCTGGTGCAGGCTGTGCATGACAATCAGATCCTAATTGTAATTGGAGAGACAGGGTCTGGGAAGACAACACAGATCACCCAGTACCTGGCAGAGGCAGGCTATACTTCCAGGGGCAAGATTGGGTGTACCCAGCCCAGAAGAGTGGCAGCTATGTCTGTGGCCAAAAGAGTCTCAGAAGAGTTTGGTTGTTGCCTTGGTCAAGAGGTGGGCTACACCATTCGATTTGAGGATTGCACCAGCCCAGAAACAGTCATCAAGTACATGACAGATGGCATGCTGCTAAGAGAGTGCCTGATTGACCCCGATCTCACTCAGTATGCCATCATTATGTTGGATGAAGCACACGAGAGGACCATTCACACAGATGTGCTCTTTGGATTGTTGAAAAAGACAGTTCAAAAACGACAAGACATGAAGCTGATTGTCACCTCAGCCACGCTGGATGCGGTGAAGTTTTCTCAGTACTTCTATGAAGCACCCATCTTCACAATCCCAGGTCGAACCTATCCAGTGGAAATTCTGTACACAAAGGAGCCGGAAACAGACTATTTGGACGCTAGCCTGATCACGGTCATGCAGATCCATCTGACAGAGCCGCCAGGTGACATCTTGGTTTTCTTGACTGGTCAGGAAGAGATCGACACTGCTTGTGAGATCCTGTATGAAAGAATGAAGTCCCTGGGACCTGATGTCCCAGAGTTGATCATCCTCCCAGTGTACTCTGCCCTTCCGAGTGAGATGCAGACCCGAATCTTTGATCCAGCTCCCCCTGGTAGCAGAAAGGTGGTGATTGCCACCAACATTGCAGAAACATCTCTGACCATCGACGGCATCTACTATGTGGTTGACCCTGGATTTGTGAAGCAGAAAGTGTACAATTCTAAAACAGGGATCGATCAGCTTGTGGTGACACCTATTTCTCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TGACCCTGGATTTGTGAAGCA
59.86
CTCAGGAATGTCGTTGGGCA
60.32
144
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TGACCCTGGATTTGTGAAGCA
59.86
ACTCAGGAATGTCGTTGGGC
60.32
145
PCR template sequence:
CGACGGCATCTACTATGTGGTTGACCCTGGATTTGTGAAGCAGAAAGTGTACAATTCTAAAACAGGGATCGATCAGCTTGTGGTGACACCTATTTCTCAGCGGAAGGCAGGCAGATCGCTGCCAACATGAGGGGGATTGGGATGATGCCCAACGACATTCCTGAGTGGAAGAAGCATGCCTTTGGGGGCAACAAAGCTTC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
7
Interacting miRNAs (#binding sites)
mmu-miR-322-5p(1), mmu-miR-15b-5p(1), mmu-miR-339-5p(1), mmu-miR-10a-5p(1), mmu-miR-218-5p(1), mmu-miR-540-3p(1), mmu-miR-1904(1)
siRNAs for circular RNA silencing
Junction sequence
ACCTATTTCTCAGCGGAAGGCAGGCA
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCDHX8_1097_ORF_1
1.206
0.352124257
0.998226833
1101
ORF sequence:
ATGAGGGGGATTGGGATGATGCCCAACGACATTCCTGAGTGGAAGAAGCATGCCTTTGGGGGCAACAAAGCTTCTTATGGGAAGAAGACCCAGATGTCAATCCTTGAGCAGAGAGAGAGCCTGCCCATCTACAAACTGAAGGAGCAGCTGGTGCAGGCTGTGCATGACAATCAGATCCTAATTGTAATTGGAGAGACAGGGTCTGGGAAGACAACACAGATCACCCAGTACCTGGCAGAGGCAGGCTATACTTCCAGGGGCAAGATTGGGTGTACCCAGCCCAGAAGAGTGGCAGCTATGTCTGTGGCCAAAAGAGTCTCAGAAGAGTTTGGTTGTTGCCTTGGTCAAGAGGTGGGCTACACCATTCGATTTGAGGATTGCACCAGCCCAGAAACAGTCATCAAGTACATGACAGATGGCATGCTGCTAAGAGAGTGCCTGATTGACCCCGATCTCACTCAGTATGCCATCATTATGTTGGATGAAGCACACGAGAGGACCATTCACACAGATGTGCTCTTTGGATTGTTGAAAAAGACAGTTCAAAAACGACAAGACATGAAGCTGATTGTCACCTCAGCCACGCTGGATGCGGTGAAGTTTTCTCAGTACTTCTATGAAGCACCCATCTTCACAATCCCAGGTCGAACCTATCCAGTGGAAATTCTGTACACAAAGGAGCCGGAAACAGACTATTTGGACGCTAGCCTGATCACGGTCATGCAGATCCATCTGACAGAGCCGCCAGGTGACATCTTGGTTTTCTTGACTGGTCAGGAAGAGATCGACACTGCTTGTGAGATCCTGTATGAAAGAATGAAGTCCCTGGGACCTGATGTCCCAGAGTTGATCATCCTCCCAGTGTACTCTGCCCTTCCGAGTGAGATGCAGACCCGAATCTTTGATCCAGCTCCCCCTGGTAGCAGAAAGGTGGTGATTGCCACCAACATTGCAGAAACATCTCTGACCATCGACGGCATCTACTATGTGGTTGACCCTGGATTTGTGAAGCAGAAAGTGTACAATTCTAAAACAGGGATCGATCAGCTTGTGGTGACACCTATTTCTCAGCGGAAGGCAGGCAGATCGCTGCCAACATGA
Peptide sequence:
MRGIGMMPNDIPEWKKHAFGGNKASYGKKTQMSILEQRESLPIYKLKEQLVQAVHDNQILIVIGETGSGKTTQITQYLAEAGYTSRGKIGCTQPRRVAAMSVAKRVSEEFGCCLGQEVGYTIRFEDCTSPETVIKYMTDGMLLRECLIDPDLTQYAIIMLDEAHERTIHTDVLFGLLKKTVQKRQDMKLIVTSATLDAVKFSQYFYEAPIFTIPGRTYPVEILYTKEPETDYLDASLITVMQIHLTEPPGDILVFLTGQEEIDTACEILYERMKSLGPDVPELIILPVYSALPSEMQTRIFDPAPPGSRKVVIATNIAETSLTIDGIYYVVDPGFVKQKVYNSKTGIDQLVVTPISQRKAGRSLPT
IRESfinder result
IRESfinder score
0.460469795
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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