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mmu_circDhx57_1522
Basic information of circular RNA
PanCircBase ID:
mmu_circDhx57_1522
mm39_Chr. Position ID:
chr17|80576180|80583464|-
Gene name:
Dhx57
Transcript:
ENSMUST00000038166.9
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
5
Exon/intron indexes:
7,6,5,4,3
Exon/intron offsets:
0,1615,4180,6012,7098
Genomic length:
7284
Exon/intron sizes in nt:
19,61,22,17,98,39,186
Flanking exon/introns:
chr17:80575068-80576180|chr17:80583464-80588624
Splice length:
1522
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
AAGAGGCACCTCTGGGGAGGAGGAAGACAGCGAGCCCCAGTGTGGCGAGGAGCAGGGCTGGCCAGCTGGACAAGAGCCTATCTTTCTTCCTGACTGCAGTCCTTGGGAATATATAGGCCCAGAAGAAGTGGAGCCTCCTGTGCCAGAATGTGCAGTCTCACCCTTGGCAGTGCAGAAACTTTCCAGGTACGGCTTCCACACTGAGCACTGTCAGCTGGCCCTGAGGATCTGTGATGGAGACTTAGGGGCGGCACTAGAGCATCTTCTCCGCCAGTGTTTCTCAGAGACGTTTGGAGAGAGGATGGCGCTCTCTGAGGCAGCTGTTTATGTGAGCTTGAATGAGTGTGTGGAGCAGAGACAGGAAGAGACGCTTGCTCTCAAGTCCATCTGCGGAGAAAAATTCATAGAAAGAATTCAGAACAGAGTCTGGACCATTGGATTGGAACTGGATTATTTGACAAACAAATTCTGCAAGTCTAAGCAAAAGGAAAGTAGCAAAAATGTACGGGACACTTCGCCTGAAACCTGTAAATTTTACCTCAAAGGAAATTGTAAATTTGGATCAAAATGCAAATTCAAACATGAAGTGCCCCCACATCAAATGATTGGGAGAGCAGAAAGAAACGTAAATGACCCTCACCTTGACGCTGATGACGACACAACCTTTATGTATGAGCTTCAGATTCGCTTTTCTAAAGACCACAAATACCCCTACCAAGCTCCCCTGGTGGCATTTTATTCCACCAATGAGAACCTACCTCTGGCTTGTCGTTTACATATTTCTGAGTTCCTTTATGGCAAGGCCTTGGAATTTGCAAAAACTTCAGAACCTGTTGTCTATTCTCTGATCACCCTTTTAGAGGAAGAGTCAGAGATTGTCAAGTTACTAACACACACTCAACATAAGTATAGCGTTCCTCCTGTGAACGTCCCTCCAGTACCTTCCGAGACCAGAATAAGTAAACCTGCCTACCGCAAACCAGTGGTCCCAAGTAACACTTTCCTTTCTAATCAAATGCTAGAAGGGGAAAGATTGTCAGAACTCGAGGAGGATGCCGATGAGGATGAAGGACCTGCATCAATTATCGTGGAGAATGAAAGCTATGTGAACCTGAAGAAGAGGAGTTACAAAAGATACGACAGGCCGGCAAAGTCCTTATTTGCTGAGAACAGTAAGATCTGCAGGCAGTTTCAGATGAAACAGGCTTCCAGACAGTTCCACGCAATTCTGCAAGAAAGACAGTTACTGCCTGCTTGGGAAGAGAGAGAGACCATTCTGAAACTGCTGAGCAAGCACCAAGTGGTTGTCATAAGTGGTATGACTGGATGTGGGAAAACCACACAGATTCCACAGTTTATCCTGGATAATTCTCTGAATGGGCCACCCGAGAGGGTGGCCAACATCATCTGTACCCAACCCCGACGGATCTCTGCAATCTCTGTTGCTGAACGGGTTGCTAAAGAAAGAGCAGAACGGGTGGGTCTGACTGTGGGATACCAGATTCGGCTGGAAAGTGTCAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AGAAAGAGCAGAACGGGTGG
59.96
ATAGGCTCTTGTCCAGCTGG
59.16
142
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TGTTGCTGAACGGGTTGCTA
60.18
ATAGGCTCTTGTCCAGCTGG
59.16
163
PCR template sequence:
ACGGATCTCTGCAATCTCTGTTGCTGAACGGGTTGCTAAAGAAAGAGCAGAACGGGTGGGTCTGACTGTGGGATACCAGATTCGGCTGGAAAGTGTCAAGAAGAGGCACCTCTGGGGAGGAGGAAGACAGCGAGCCCCAGTGTGGCGAGGAGCAGGGCTGGCCAGCTGGACAAGAGCCTATCTTTCTTCCTGACTGCAGT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
19
Interacting miRNAs (#binding sites)
mmu-miR-188-3p(2), mmu-miR-1904(2), mmu-miR-200a-3p(1), mmu-miR-141-3p(1), mmu-miR-106a-5p(1), mmu-miR-200c-3p(1), mmu-miR-26a-5p(1), mmu-miR-205-5p(1), mmu-miR-18a-5p(1), mmu-miR-26b-5p(1), mmu-miR-29b-1-5p(1), mmu-miR-127-5p(1), mmu-miR-324-3p(1), mmu-miR-383-5p(1), mmu-miR-98-5p(1), mmu-miR-802-5p(1), mmu-miR-129-5p(1), mmu-miR-5101(1), mmu-miR-574-5p(1)
siRNAs for circular RNA silencing
Junction sequence
GGAAAGTGTCAAGAAGAGGCACCTCT
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCDHX57_1522_ORF_1
0.7879
0.162850521
0.998165285
1314
ORF sequence:
ATGGCGCTCTCTGAGGCAGCTGTTTATGTGAGCTTGAATGAGTGTGTGGAGCAGAGACAGGAAGAGACGCTTGCTCTCAAGTCCATCTGCGGAGAAAAATTCATAGAAAGAATTCAGAACAGAGTCTGGACCATTGGATTGGAACTGGATTATTTGACAAACAAATTCTGCAAGTCTAAGCAAAAGGAAAGTAGCAAAAATGTACGGGACACTTCGCCTGAAACCTGTAAATTTTACCTCAAAGGAAATTGTAAATTTGGATCAAAATGCAAATTCAAACATGAAGTGCCCCCACATCAAATGATTGGGAGAGCAGAAAGAAACGTAAATGACCCTCACCTTGACGCTGATGACGACACAACCTTTATGTATGAGCTTCAGATTCGCTTTTCTAAAGACCACAAATACCCCTACCAAGCTCCCCTGGTGGCATTTTATTCCACCAATGAGAACCTACCTCTGGCTTGTCGTTTACATATTTCTGAGTTCCTTTATGGCAAGGCCTTGGAATTTGCAAAAACTTCAGAACCTGTTGTCTATTCTCTGATCACCCTTTTAGAGGAAGAGTCAGAGATTGTCAAGTTACTAACACACACTCAACATAAGTATAGCGTTCCTCCTGTGAACGTCCCTCCAGTACCTTCCGAGACCAGAATAAGTAAACCTGCCTACCGCAAACCAGTGGTCCCAAGTAACACTTTCCTTTCTAATCAAATGCTAGAAGGGGAAAGATTGTCAGAACTCGAGGAGGATGCCGATGAGGATGAAGGACCTGCATCAATTATCGTGGAGAATGAAAGCTATGTGAACCTGAAGAAGAGGAGTTACAAAAGATACGACAGGCCGGCAAAGTCCTTATTTGCTGAGAACAGTAAGATCTGCAGGCAGTTTCAGATGAAACAGGCTTCCAGACAGTTCCACGCAATTCTGCAAGAAAGACAGTTACTGCCTGCTTGGGAAGAGAGAGAGACCATTCTGAAACTGCTGAGCAAGCACCAAGTGGTTGTCATAAGTGGTATGACTGGATGTGGGAAAACCACACAGATTCCACAGTTTATCCTGGATAATTCTCTGAATGGGCCACCCGAGAGGGTGGCCAACATCATCTGTACCCAACCCCGACGGATCTCTGCAATCTCTGTTGCTGAACGGGTTGCTAAAGAAAGAGCAGAACGGGTGGGTCTGACTGTGGGATACCAGATTCGGCTGGAAAGTGTCAAGAAGAGGCACCTCTGGGGAGGAGGAAGACAGCGAGCCCCAGTGTGGCGAGGAGCAGGGCTGGCCAGCTGGACAAGAGCCTATCTTTCTTCCTGA
Peptide sequence:
MALSEAAVYVSLNECVEQRQEETLALKSICGEKFIERIQNRVWTIGLELDYLTNKFCKSKQKESSKNVRDTSPETCKFYLKGNCKFGSKCKFKHEVPPHQMIGRAERNVNDPHLDADDDTTFMYELQIRFSKDHKYPYQAPLVAFYSTNENLPLACRLHISEFLYGKALEFAKTSEPVVYSLITLLEEESEIVKLLTHTQHKYSVPPVNVPPVPSETRISKPAYRKPVVPSNTFLSNQMLEGERLSELEEDADEDEGPASIIVENESYVNLKKRSYKRYDRPAKSLFAENSKICRQFQMKQASRQFHAILQERQLLPAWEERETILKLLSKHQVVVISGMTGCGKTTQIPQFILDNSLNGPPERVANIICTQPRRISAISVAERVAKERAERVGLTVGYQIRLESVKKRHLWGGGRQRAPVWRGAGLASWTRAYLSS
IRESfinder result
IRESfinder score
0.565627169
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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