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mmu_circDhx33_585
Basic information of circular RNA
PanCircBase ID:
mmu_circDhx33_585
mm39_Chr. Position ID:
chr11|70886713|70890774|-
Gene name:
Dhx33
Transcript:
ENSMUST00000108527.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
5,4,3
Exon/intron offsets:
0,3482,3833
Genomic length:
4061
Exon/intron sizes in nt:
18,61,71,228
Flanking exon/introns:
chr11:70886076-70886713|chr11:70890774-70892344
Splice length:
585
Other database IDs:
circAtlas: mmu-Dhx33_0005
circAtlas: hsa-DHX33_0011
Human Ortholog IDs:
Mature spliced Sequence:
GTTGGCTATACAGTGCGCTTTGAGGATGTCACCTCAGAAGACACCAGGATCAAGTTCCTGACAGATGGCATGCTTCTGCGTGAAGCAATTTCAGACTCCTTGCTTCGGAAGTACAGCTGTGTCATTTTGGACGAGGCCCATGAGCGGACTATTCACACAGATGTGCTCTTTGGAGTGGTGAAGACTGCACAGAAGAGGCGAAAGGAGCTAGGGAAACTGCCTCTCAAAGTGATTGTGATGTCAGCTACCATGGATGTCGACCTGTTTTCTCAGTACTTCAATAGAGCCCCTGTCCTTTACCTTGAGGGTCGGCAGCATCCAATCCAGATCTTCTATACCAAACAGCCTCAGCAGGATTACCTGCATGCAGCCCTTGTCTCAGTCTTCCAGATCCACCAGGAAGCTCCTGCTTCTCAAGACATCCTGGTGTTCCTCACTGGTCAGGAGGAGATCGAAGCAATGAGCAAGACCTGCAGAGACATCGCAAGGCACCTCCCAGATGGCTGCCCTTCAATGCTCGTCCTTCCTCTGTACGCCTCCCTGCCCTATTCGCAGCAGCTTCGAGTCTTTCAAGGGGCCCCAAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CTCGTCCTTCCTCTGTACGC
59.9
GCAGAAGCATGCCATCTGTC
59.62
148
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GCTCGTCCTTCCTCTGTACG
59.9
GCAGAAGCATGCCATCTGTC
59.62
149
PCR template sequence:
AAGGCACCTCCCAGATGGCTGCCCTTCAATGCTCGTCCTTCCTCTGTACGCCTCCCTGCCCTATTCGCAGCAGCTTCGAGTCTTTCAAGGGGCCCCAAAGGTTGGCTATACAGTGCGCTTTGAGGATGTCACCTCAGAAGACACCAGGATCAAGTTCCTGACAGATGGCATGCTTCTGCGTGAAGCAATTTCAGACTCCT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
2
Interacting miRNAs (#binding sites)
mmu-miR-370-3p(1), mmu-miR-425-5p(1)
siRNAs for circular RNA silencing
Junction sequence
AGGGGCCCCAAAGGTTGGCTATACAG
siRNA sets
TGTATAGCCAACCTTTGGG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCDHX33_585_ORF_1
1.2805
0.353894107
0.998503195
1101
ORF sequence:
ATGCTTCTGCGTGAAGCAATTTCAGACTCCTTGCTTCGGAAGTACAGCTGTGTCATTTTGGACGAGGCCCATGAGCGGACTATTCACACAGATGTGCTCTTTGGAGTGGTGAAGACTGCACAGAAGAGGCGAAAGGAGCTAGGGAAACTGCCTCTCAAAGTGATTGTGATGTCAGCTACCATGGATGTCGACCTGTTTTCTCAGTACTTCAATAGAGCCCCTGTCCTTTACCTTGAGGGTCGGCAGCATCCAATCCAGATCTTCTATACCAAACAGCCTCAGCAGGATTACCTGCATGCAGCCCTTGTCTCAGTCTTCCAGATCCACCAGGAAGCTCCTGCTTCTCAAGACATCCTGGTGTTCCTCACTGGTCAGGAGGAGATCGAAGCAATGAGCAAGACCTGCAGAGACATCGCAAGGCACCTCCCAGATGGCTGCCCTTCAATGCTCGTCCTTCCTCTGTACGCCTCCCTGCCCTATTCGCAGCAGCTTCGAGTCTTTCAAGGGGCCCCAAAGGTTGGCTATACAGTGCGCTTTGAGGATGTCACCTCAGAAGACACCAGGATCAAGTTCCTGACAGATGGCATGCTTCTGCGTGAAGCAATTTCAGACTCCTTGCTTCGGAAGTACAGCTGTGTCATTTTGGACGAGGCCCATGAGCGGACTATTCACACAGATGTGCTCTTTGGAGTGGTGAAGACTGCACAGAAGAGGCGAAAGGAGCTAGGGAAACTGCCTCTCAAAGTGATTGTGATGTCAGCTACCATGGATGTCGACCTGTTTTCTCAGTACTTCAATAGAGCCCCTGTCCTTTACCTTGAGGGTCGGCAGCATCCAATCCAGATCTTCTATACCAAACAGCCTCAGCAGGATTACCTGCATGCAGCCCTTGTCTCAGTCTTCCAGATCCACCAGGAAGCTCCTGCTTCTCAAGACATCCTGGTGTTCCTCACTGGTCAGGAGGAGATCGAAGCAATGAGCAAGACCTGCAGAGACATCGCAAGGCACCTCCCAGATGGCTGCCCTTCAATGCTCGTCCTTCCTCTGTACGCCTCCCTGCCCTATTCGCAGCAGCTTCGAGTCTTTCAAGGGGCCCCAAAG
Peptide sequence:
MLLREAISDSLLRKYSCVILDEAHERTIHTDVLFGVVKTAQKRRKELGKLPLKVIVMSATMDVDLFSQYFNRAPVLYLEGRQHPIQIFYTKQPQQDYLHAALVSVFQIHQEAPASQDILVFLTGQEEIEAMSKTCRDIARHLPDGCPSMLVLPLYASLPYSQQLRVFQGAPKVGYTVRFEDVTSEDTRIKFLTDGMLLREAISDSLLRKYSCVILDEAHERTIHTDVLFGVVKTAQKRRKELGKLPLKVIVMSATMDVDLFSQYFNRAPVLYLEGRQHPIQIFYTKQPQQDYLHAALVSVFQIHQEAPASQDILVFLTGQEEIEAMSKTCRDIARHLPDGCPSMLVLPLYASLPYSQQLRVFQGAPK
IRESfinder result
IRESfinder score
0.531653942
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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