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mmu_circDhtkd1_2418
Basic information of circular RNA
PanCircBase ID:
mmu_circDhtkd1_2418
mm39_Chr. Position ID:
chr2|5907139|5937353|-
Gene name:
Dhtkd1
Transcript:
ENSMUST00000095147.9
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
14
Exon/intron indexes:
15,14,13,12,11,10,9,8,7,6,5,4,3,2
Exon/intron offsets:
0,639,1631,7973,9485,11183,12481,15344,17111,19336,21540,27309,28446,30058
Genomic length:
30214
Exon/intron sizes in nt:
1,70,83,16,51,07,15,11,00,00,00,00,00,00,00,00,00,00,00,000
Flanking exon/introns:
chr2:5904807-5907139|chr2:5937353-5947325
Splice length:
2418
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
TTGACCATGGCCTTGCCAGGCTGGTGACAGTGTATTGTGAGCATGGCCACAAAGCTGCCCAGATCAACCCTCTCTTCCCTGGACAAGCCCTCCTGGATACTGTGCCTGAAATCCAAGCTCTTGTCCGGACACTCCAGGGACCCTTCACTACCACAGGACTGTTAAACCTGGGCAAAGAAGCAGCTTCTCTTGAGGAAGTATTGGCCTATCTGAACCATATCTACTGTGGCCCAATTTCTATCGAAACCGCCCAGCTTCAGAGCCAGGAAGAGAGAGACTGGTTTGCCAGAAGGTTTGAGGAACTGAAAAAGGAGACGTTCACCACAGAAGAACGAAAATACCTGTCCAAACTATTGCTAGAGTCTCAGGAGTTTGACCACTTTTTGGCCACCAAGTTTGCCACGGTGAAGAGATACGGCGGTGAAGGGGCAGAGAGCATGATGGGCTTTTTTCATGAGTTGCTGAAATTGTCAGCCTATGGCGGTATAACCGACATCATTATCGGGATGCCCCATAGAGGGAGGTTGAATTTGTTGACAGGCCTTCTGCAGTTACCTCCGGAGCTGATGTTCCGTAAAATGCGAGGCTTAAGCGAGTTTCCAGAGAATGTTGCCACCATTGGTGATGTCCTGTCCCACCTGACCTCCTCAGTGGACCTAGACTTCGGTGCCCATCAACCCCTCCATGTGACAATGCTGCCCAACCCCTCACATCTGGAAGCTGTTAACCCCGTGGCTGTAGGGAAAACTCGTGGCAGGCAGCAGTCTCGGGAAGATGGGGACTACTCCCCGAATGGTTCTGCTCAGCCTGGGGACAAAGTCATATGCCTGCAGGTCCACGGTGACGCTTCCTTCTGCGGTCAGGGGATTGTCCTTGAGACGTTCACACTCTCTAATCTTCCACACTTCAGAATTGGTGGGAGCATCCATCTAATTGTCAATAACCAGCTGGGCTACACCACTCCTGCAGAGAGAGGAAGGTCGTCCTTATACAGCTCTGATATCGGAAAGCTCGTAGGCTGTGCCATCATCCATGTCAATGGAGACAGCCCAGAGGAAGTGGTCCGAGCGACACGCCTGGCTTTTGAATATCAGCGCCAGTTCCGCAAGGATGTGATTGTTGATTTGTTATGCTATAGGCAGTGGGGTCATAACGAGCTAGACGAGCCTTTCTTCACCAACCCAGTCATGTACAAAATCATCAGAGCCCGCAAGAGCATTCCTGATACCTACGCAGAGCACCTCATTGCCAGTGGACTCATGACCCAGGAGGAGGTTTCCGACATAAAAACCTCCTACTATACCAAGTTAAATGACCACTTGGCCAATGTGGCCCACTACAGCCCCCCGGCCACTAACCTGCAGGCCCGCTGGCAGGGCCTGGTGCAGCCAGAAGCATGTGTCACCACCTGGGACACAGGGGTGCCTCTAGAGCTGCTGCGGTTTATTGGTGTGAAGTCCGTGGAGGTTCCAGAGGAACTGCAGGTGCACAGCCACCTTCTAAAGATGTATGTTCAGTCAAGAATGGAGAAGGTGAAGAATGGAAGCGGTCTAGACTGGGCCACCGCAGAGACACTTGCCTTGGGCTCCTTACTCGCTCAAGGTTTCAATGTTCGTCTGAGCGGCCAAGATGTTGGCCGTGGGACTTTTAGTCAAAGGCATGCCATGGTGGTTTGCCAGGACACTGATGATGCCTACATCCCGCTGAACCACATGGACCCCAACCAGAAGGGTTTTCTGGAGGTTAGCAACAGCCCCCTGTCAGAAGAGGCTGTCCTGGGATTTGAATATGGGATGAGCATCGAAAGCCCCACGTTGCTGCCCCTCTGGGAAGCTCAGTTTGGCGATTTCTTCAATGGAGCCCAGATCATCTTTGACACATTCATCTCAGGAGGAGAGGCCAAGTGGCTCCTGCAGAGTGGCCTCGTCATCCTCCTTCCACATGGCTATGATGGAGCTGGGCCTGAGCACTCATCTTGTCGGATAGAGCGCTTCTTGCAGATGTGCGATAGTGCAGAAGAAGGTGTGGACAGCGACACTGTGAACATGTTTGTGGTACACCCGACCACCCCTGCTCAGTACTTCCATTTGCTAAGAAGGCAGATGATTCGGAACTTCAGAAAACCTCTCATTGTTGCATCTCCAAAAATGTTACTCAGGTACCCTGCAGCTGTGTCAACTCTTGAGGAAATGGCACCAGGAACAGCATTCAAACCTGTCATTGGTGATTCATCAGTAGACCCAAAAAATGTTAAAACCCTCATATTCTGCTCCGGCAAACATTTTTATGCCCTGCTGAAGCAAAGAGAGTCTCTGGGGACTAAGAAACATGATTTTGCCATCATTCGACTAGAGGAGCTCTGCCCTTTCCCGCTGGATGCATTACAACAAGAGATGAGCAAATATAAACATGTTCGAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GACTAGAGGAGCTCTGCCCT
60.11
TATCCAGGAGGGCTTGTCCA
59.96
171
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GACTAGAGGAGCTCTGCCCT
60.11
GAGAGGGTTGATCTGGGCAG
59.82
146
PCR template sequence:
ACTAAGAAACATGATTTTGCCATCATTCGACTAGAGGAGCTCTGCCCTTTCCCGCTGGATGCATTACAACAAGAGATGAGCAAATATAAACATGTTCGAGTTGACCATGGCCTTGCCAGGCTGGTGACAGTGTATTGTGAGCATGGCCACAAAGCTGCCCAGATCAACCCTCTCTTCCCTGGACAAGCCCTCCTGGATAC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
18
Interacting miRNAs (#binding sites)
mmu-miR-136-5p(2), mmu-miR-7a-1-3p(2), mmu-miR-27b-3p(1), mmu-miR-27a-3p(1), mmu-miR-103-3p(1), mmu-miR-122-5p(1), mmu-miR-324-5p(1), mmu-miR-326-3p(1), mmu-miR-214-3p(1), mmu-miR-127-5p(1), mmu-miR-182-5p(1), mmu-miR-362-5p(1), mmu-miR-107-3p(1), mmu-miR-532-5p(1), mmu-miR-3065-5p(1), mmu-miR-3470b(1), mmu-miR-574-5p(1), mmu-miR-296-3p(1)
siRNAs for circular RNA silencing
Junction sequence
AAACATGTTCGAGTTGACCATGGCCT
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCDHTKD1_2418_ORF_1
1.1836
0.272196085
1
4398
ORF sequence:
ATGATGGGCTTTTTTCATGAGTTGCTGAAATTGTCAGCCTATGGCGGTATAACCGACATCATTATCGGGATGCCCCATAGAGGGAGGTTGAATTTGTTGACAGGCCTTCTGCAGTTACCTCCGGAGCTGATGTTCCGTAAAATGCGAGGCTTAAGCGAGTTTCCAGAGAATGTTGCCACCATTGGTGATGTCCTGTCCCACCTGACCTCCTCAGTGGACCTAGACTTCGGTGCCCATCAACCCCTCCATGTGACAATGCTGCCCAACCCCTCACATCTGGAAGCTGTTAACCCCGTGGCTGTAGGGAAAACTCGTGGCAGGCAGCAGTCTCGGGAAGATGGGGACTACTCCCCGAATGGTTCTGCTCAGCCTGGGGACAAAGTCATATGCCTGCAGGTCCACGGTGACGCTTCCTTCTGCGGTCAGGGGATTGTCCTTGAGACGTTCACACTCTCTAATCTTCCACACTTCAGAATTGGTGGGAGCATCCATCTAATTGTCAATAACCAGCTGGGCTACACCACTCCTGCAGAGAGAGGAAGGTCGTCCTTATACAGCTCTGATATCGGAAAGCTCGTAGGCTGTGCCATCATCCATGTCAATGGAGACAGCCCAGAGGAAGTGGTCCGAGCGACACGCCTGGCTTTTGAATATCAGCGCCAGTTCCGCAAGGATGTGATTGTTGATTTGTTATGCTATAGGCAGTGGGGTCATAACGAGCTAGACGAGCCTTTCTTCACCAACCCAGTCATGTACAAAATCATCAGAGCCCGCAAGAGCATTCCTGATACCTACGCAGAGCACCTCATTGCCAGTGGACTCATGACCCAGGAGGAGGTTTCCGACATAAAAACCTCCTACTATACCAAGTTAAATGACCACTTGGCCAATGTGGCCCACTACAGCCCCCCGGCCACTAACCTGCAGGCCCGCTGGCAGGGCCTGGTGCAGCCAGAAGCATGTGTCACCACCTGGGACACAGGGGTGCCTCTAGAGCTGCTGCGGTTTATTGGTGTGAAGTCCGTGGAGGTTCCAGAGGAACTGCAGGTGCACAGCCACCTTCTAAAGATGTATGTTCAGTCAAGAATGGAGAAGGTGAAGAATGGAAGCGGTCTAGACTGGGCCACCGCAGAGACACTTGCCTTGGGCTCCTTACTCGCTCAAGGTTTCAATGTTCGTCTGAGCGGCCAAGATGTTGGCCGTGGGACTTTTAGTCAAAGGCATGCCATGGTGGTTTGCCAGGACACTGATGATGCCTACATCCCGCTGAACCACATGGACCCCAACCAGAAGGGTTTTCTGGAGGTTAGCAACAGCCCCCTGTCAGAAGAGGCTGTCCTGGGATTTGAATATGGGATGAGCATCGAAAGCCCCACGTTGCTGCCCCTCTGGGAAGCTCAGTTTGGCGATTTCTTCAATGGAGCCCAGATCATCTTTGACACATTCATCTCAGGAGGAGAGGCCAAGTGGCTCCTGCAGAGTGGCCTCGTCATCCTCCTTCCACATGGCTATGATGGAGCTGGGCCTGAGCACTCATCTTGTCGGATAGAGCGCTTCTTGCAGATGTGCGATAGTGCAGAAGAAGGTGTGGACAGCGACACTGTGAACATGTTTGTGGTACACCCGACCACCCCTGCTCAGTACTTCCATTTGCTAAGAAGGCAGATGATTCGGAACTTCAGAAAACCTCTCATTGTTGCATCTCCAAAAATGTTACTCAGGTACCCTGCAGCTGTGTCAACTCTTGAGGAAATGGCACCAGGAACAGCATTCAAACCTGTCATTGGTGATTCATCAGTAGACCCAAAAAATGTTAAAACCCTCATATTCTGCTCCGGCAAACATTTTTATGCCCTGCTGAAGCAAAGAGAGTCTCTGGGGACTAAGAAACATGATTTTGCCATCATTCGACTAGAGGAGCTCTGCCCTTTCCCGCTGGATGCATTACAACAAGAGATGAGCAAATATAAACATGTTCGAGTTGACCATGGCCTTGCCAGGCTGGTGACAGTGTATTGTGAGCATGGCCACAAAGCTGCCCAGATCAACCCTCTCTTCCCTGGACAAGCCCTCCTGGATACTGTGCCTGAAATCCAAGCTCTTGTCCGGACACTCCAGGGACCCTTCACTACCACAGGACTGTTAAACCTGGGCAAAGAAGCAGCTTCTCTTGAGGAAGTATTGGCCTATCTGAACCATATCTACTGTGGCCCAATTTCTATCGAAACCGCCCAGCTTCAGAGCCAGGAAGAGAGAGACTGGTTTGCCAGAAGGTTTGAGGAACTGAAAAAGGAGACGTTCACCACAGAAGAACGAAAATACCTGTCCAAACTATTGCTAGAGTCTCAGGAGTTTGACCACTTTTTGGCCACCAAGTTTGCCACGGTGAAGAGATACGGCGGTGAAGGGGCAGAGAGCATGATGGGCTTTTTTCATGAGTTGCTGAAATTGTCAGCCTATGGCGGTATAACCGACATCATTATCGGGATGCCCCATAGAGGGAGGTTGAATTTGTTGACAGGCCTTCTGCAGTTACCTCCGGAGCTGATGTTCCGTAAAATGCGAGGCTTAAGCGAGTTTCCAGAGAATGTTGCCACCATTGGTGATGTCCTGTCCCACCTGACCTCCTCAGTGGACCTAGACTTCGGTGCCCATCAACCCCTCCATGTGACAATGCTGCCCAACCCCTCACATCTGGAAGCTGTTAACCCCGTGGCTGTAGGGAAAACTCGTGGCAGGCAGCAGTCTCGGGAAGATGGGGACTACTCCCCGAATGGTTCTGCTCAGCCTGGGGACAAAGTCATATGCCTGCAGGTCCACGGTGACGCTTCCTTCTGCGGTCAGGGGATTGTCCTTGAGACGTTCACACTCTCTAATCTTCCACACTTCAGAATTGGTGGGAGCATCCATCTAATTGTCAATAACCAGCTGGGCTACACCACTCCTGCAGAGAGAGGAAGGTCGTCCTTATACAGCTCTGATATCGGAAAGCTCGTAGGCTGTGCCATCATCCATGTCAATGGAGACAGCCCAGAGGAAGTGGTCCGAGCGACACGCCTGGCTTTTGAATATCAGCGCCAGTTCCGCAAGGATGTGATTGTTGATTTGTTATGCTATAGGCAGTGGGGTCATAACGAGCTAGACGAGCCTTTCTTCACCAACCCAGTCATGTACAAAATCATCAGAGCCCGCAAGAGCATTCCTGATACCTACGCAGAGCACCTCATTGCCAGTGGACTCATGACCCAGGAGGAGGTTTCCGACATAAAAACCTCCTACTATACCAAGTTAAATGACCACTTGGCCAATGTGGCCCACTACAGCCCCCCGGCCACTAACCTGCAGGCCCGCTGGCAGGGCCTGGTGCAGCCAGAAGCATGTGTCACCACCTGGGACACAGGGGTGCCTCTAGAGCTGCTGCGGTTTATTGGTGTGAAGTCCGTGGAGGTTCCAGAGGAACTGCAGGTGCACAGCCACCTTCTAAAGATGTATGTTCAGTCAAGAATGGAGAAGGTGAAGAATGGAAGCGGTCTAGACTGGGCCACCGCAGAGACACTTGCCTTGGGCTCCTTACTCGCTCAAGGTTTCAATGTTCGTCTGAGCGGCCAAGATGTTGGCCGTGGGACTTTTAGTCAAAGGCATGCCATGGTGGTTTGCCAGGACACTGATGATGCCTACATCCCGCTGAACCACATGGACCCCAACCAGAAGGGTTTTCTGGAGGTTAGCAACAGCCCCCTGTCAGAAGAGGCTGTCCTGGGATTTGAATATGGGATGAGCATCGAAAGCCCCACGTTGCTGCCCCTCTGGGAAGCTCAGTTTGGCGATTTCTTCAATGGAGCCCAGATCATCTTTGACACATTCATCTCAGGAGGAGAGGCCAAGTGGCTCCTGCAGAGTGGCCTCGTCATCCTCCTTCCACATGGCTATGATGGAGCTGGGCCTGAGCACTCATCTTGTCGGATAGAGCGCTTCTTGCAGATGTGCGATAGTGCAGAAGAAGGTGTGGACAGCGACACTGTGAACATGTTTGTGGTACACCCGACCACCCCTGCTCAGTACTTCCATTTGCTAAGAAGGCAGATGATTCGGAACTTCAGAAAACCTCTCATTGTTGCATCTCCAAAAATGTTACTCAGGTACCCTGCAGCTGTGTCAACTCTTGAGGAAATGGCACCAGGAACAGCATTCAAACCTGTCATTGGTGATTCATCAGTAGACCCAAAAAATGTTAAAACCCTCATATTCTGCTCCGGCAAACATTTTTATGCCCTGCTGAAGCAAAGAGAGTCTCTGGGGACTAAGAAACATGATTTTGCCATCATTCGACTAGAGGAGCTCTGCCCTTTCCCGCTGGATGCATTACAACAAGAGATGAGCAAATATAAACATGTTCGA
Peptide sequence:
MMGFFHELLKLSAYGGITDIIIGMPHRGRLNLLTGLLQLPPELMFRKMRGLSEFPENVATIGDVLSHLTSSVDLDFGAHQPLHVTMLPNPSHLEAVNPVAVGKTRGRQQSREDGDYSPNGSAQPGDKVICLQVHGDASFCGQGIVLETFTLSNLPHFRIGGSIHLIVNNQLGYTTPAERGRSSLYSSDIGKLVGCAIIHVNGDSPEEVVRATRLAFEYQRQFRKDVIVDLLCYRQWGHNELDEPFFTNPVMYKIIRARKSIPDTYAEHLIASGLMTQEEVSDIKTSYYTKLNDHLANVAHYSPPATNLQARWQGLVQPEACVTTWDTGVPLELLRFIGVKSVEVPEELQVHSHLLKMYVQSRMEKVKNGSGLDWATAETLALGSLLAQGFNVRLSGQDVGRGTFSQRHAMVVCQDTDDAYIPLNHMDPNQKGFLEVSNSPLSEEAVLGFEYGMSIESPTLLPLWEAQFGDFFNGAQIIFDTFISGGEAKWLLQSGLVILLPHGYDGAGPEHSSCRIERFLQMCDSAEEGVDSDTVNMFVVHPTTPAQYFHLLRRQMIRNFRKPLIVASPKMLLRYPAAVSTLEEMAPGTAFKPVIGDSSVDPKNVKTLIFCSGKHFYALLKQRESLGTKKHDFAIIRLEELCPFPLDALQQEMSKYKHVRVDHGLARLVTVYCEHGHKAAQINPLFPGQALLDTVPEIQALVRTLQGPFTTTGLLNLGKEAASLEEVLAYLNHIYCGPISIETAQLQSQEERDWFARRFEELKKETFTTEERKYLSKLLLESQEFDHFLATKFATVKRYGGEGAESMMGFFHELLKLSAYGGITDIIIGMPHRGRLNLLTGLLQLPPELMFRKMRGLSEFPENVATIGDVLSHLTSSVDLDFGAHQPLHVTMLPNPSHLEAVNPVAVGKTRGRQQSREDGDYSPNGSAQPGDKVICLQVHGDASFCGQGIVLETFTLSNLPHFRIGGSIHLIVNNQLGYTTPAERGRSSLYSSDIGKLVGCAIIHVNGDSPEEVVRATRLAFEYQRQFRKDVIVDLLCYRQWGHNELDEPFFTNPVMYKIIRARKSIPDTYAEHLIASGLMTQEEVSDIKTSYYTKLNDHLANVAHYSPPATNLQARWQGLVQPEACVTTWDTGVPLELLRFIGVKSVEVPEELQVHSHLLKMYVQSRMEKVKNGSGLDWATAETLALGSLLAQGFNVRLSGQDVGRGTFSQRHAMVVCQDTDDAYIPLNHMDPNQKGFLEVSNSPLSEEAVLGFEYGMSIESPTLLPLWEAQFGDFFNGAQIIFDTFISGGEAKWLLQSGLVILLPHGYDGAGPEHSSCRIERFLQMCDSAEEGVDSDTVNMFVVHPTTPAQYFHLLRRQMIRNFRKPLIVASPKMLLRYPAAVSTLEEMAPGTAFKPVIGDSSVDPKNVKTLIFCSGKHFYALLKQRESLGTKKHDFAIIRLEELCPFPLDALQQEMSKYKHVR
IRESfinder result
IRESfinder score
0.460647071
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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