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mmu_circDcun1d4_364
Basic information of circular RNA
PanCircBase ID:
mmu_circDcun1d4_364
mm39_Chr. Position ID:
chr5|73679995|73701498|+
Gene name:
Dcun1d4
Transcript:
ENSMUST00000087181.12
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
4
Exon/intron indexes:
6,7,8,9
Exon/intron offsets:
0,8851,11953,21394
Genomic length:
21503
Exon/intron sizes in nt:
92,71,92,109
Flanking exon/introns:
chr5:73677573-73679995|chr5:73701498-73712701
Splice length:
364
Other database IDs:
CIRCpedia: MMU_CIRCpedia_28469, circAtlas: mmu-Dcun1d4_0015
Human Ortholog IDs:
Mature spliced Sequence:
CATGTATAGGAAATACGAGTCAACTAGAATAAAGACTGAAGAAGAGGCTTTCTCCAGTAAGCGGTGCCTGGAGTGGTTTTATGAATATGCAGGAACTGAGGATGCCGTAGGTCCCGAAGGCATGGAGAAGTTCTGTGAAGACATTGGTGTTGAACCAGAAAACGTAGTTATGCTTGTCCTAGCTTGGAAATTGGATGCACAAAACATGGGCTATTTTACTCTACAGGAGTGGCTGAAAGGAATGACTTCCCTACAATGTGATACAACAGAAAAACTCAGAACTACTTTGGATTACTTAAGATCATTATTAAACGATACAACAAACTTTAAGCTCATTTACAGATACGCGTTTGACTTTGCACGG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
ACAGAAAAACTCAGAACTACTTTGGA
58.89
ACCGCTTACTGGAGAAAGCC
60.04
164
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
ACAGAAAAACTCAGAACTACTTTGG
57.68
ACCGCTTACTGGAGAAAGCC
60.04
164
PCR template sequence:
AACAGAAAAACTCAGAACTACTTTGGATTACTTAAGATCATTATTAAACGATACAACAAACTTTAAGCTCATTTACAGATACGCGTTTGACTTTGCACGGCATGTATAGGAAATACGAGTCAACTAGAATAAAGACTGAAGAAGAGGCTTTCTCCAGTAAGCGGTGCCTGGAGTGGTTTTATGAATATGCAGGAACTGAG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
5
Interacting miRNAs (#binding sites)
mmu-miR-23b-3p(1), mmu-miR-19b-3p(1), mmu-miR-19a-3p(1), mmu-miR-23a-3p(1), mmu-miR-207(1)
siRNAs for circular RNA silencing
Junction sequence
TGACTTTGCACGGCATGTATAGGAAA
siRNA sets
TTTCCTATACATGCCGTGC, ATACATGCCGTGCAAAGTC, TTCCTATACATGCCGTGCA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCDCUN1D4_364_ORF_1
0.7476
0.04570586
0.269229419
372
ORF sequence:
ATGTATAGGAAATACGAGTCAACTAGAATAAAGACTGAAGAAGAGGCTTTCTCCAGTAAGCGGTGCCTGGAGTGGTTTTATGAATATGCAGGAACTGAGGATGCCGTAGGTCCCGAAGGCATGGAGAAGTTCTGTGAAGACATTGGTGTTGAACCAGAAAACGTAGTTATGCTTGTCCTAGCTTGGAAATTGGATGCACAAAACATGGGCTATTTTACTCTACAGGAGTGGCTGAAAGGAATGACTTCCCTACAATGTGATACAACAGAAAAACTCAGAACTACTTTGGATTACTTAAGATCATTATTAAACGATACAACAAACTTTAAGCTCATTTACAGATACGCGTTTGACTTTGCACGGCATGTATAG
Peptide sequence:
MYRKYESTRIKTEEEAFSSKRCLEWFYEYAGTEDAVGPEGMEKFCEDIGVEPENVVMLVLAWKLDAQNMGYFTLQEWLKGMTSLQCDTTEKLRTTLDYLRSLLNDTTNFKLIYRYAFDFARHV
IRESfinder result
IRESfinder score
0.625782462
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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