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mmu_circDclre1c_910
Basic information of circular RNA
PanCircBase ID:
mmu_circDclre1c_910
mm39_Chr. Position ID:
chr2|3434783|3451410|+
Gene name:
Dclre1c
Transcript:
ENSMUST00000100463.10
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
10
Exon/intron indexes:
4,5,6,7,8,9,10,11,12,13
Exon/intron offsets:
0,2691,3242,3929,4205,7011,9907,10454,13327,16532
Genomic length:
16627
Exon/intron sizes in nt:
60,56,102,73,141,102,137,55,89,95
Flanking exon/introns:
chr2:3430389-3434783|chr2:3451410-3458580
Splice length:
910
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
ATAACAATTGAAATTGAAACTCCTACGCAGATATCTTTAGTTGATGAGGCTTCGGGTGAGAAGGAAGAGGTTGTTGTGACTCTCTTACCAGCTGGTCACTGCCCAGGATCAGTTATGTTTTTATTTCAGGGCAGTAATGGAACTGTCTTATACACAGGAGACTTCAGACTGGCAAAAGGAGAAGCTTCCAGAATGGAGCTTCTGCACTCTGGAGGCAGAGTAAAAGACATCCAAAGTGTGTATTTAGACACGACTTTCTGTGACCCAAGGTTTTATCAGATCCCAAGTCGTGAGCAGTGTTTGAGGGGCATTTTGGAGCTGGTTCGGAGCTGGGTCACTAGGAGTCCGCACCACGTCGTGTGGCTGAACTGTAAAGCAGCTTATGGCTACGAGTATTTATTCACCAACCTAAGCGAGGAGCTGGGAGTTCAGGTTCATGTGGACAAGCTGGACATGTTTAAAAACATGCCTGATATCCTGCACCATCTCACAACGGACAGAAACACCCAGATCCACGCCTGCCGCCACCCAAAGGCAGAAGAGTGTTTTCAGTGGAATAAACTACCCTGTGGTATAACTTCCCAAAACAAAACTGCACTCCACACAATCAGCATCAAGCCATCTACCATGTGGTTTGGAGAGAGGACCAGAAAAACCAACGTGATCGTTAGGACTGGAGAGAGCTCATACAGAGCTTGCTTCTCTTTTCACTCCTCCTTCAGTGAGATTAAAGATTTTTTGAGCTACATCTGCCCAGTGAATGTGTATCCAAATGTCATCCCAGTTGGCCTCACTGTGGATAAGGTCATGGACGTTTTAAAGCCTCTGTGCCGGTCTCCCCAAAGTGTTGAGCCAAAGTACAAACCGCTTGGAAAATTGAAGAGAGCCAGAACAATCCATCTTGACTCGG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TTTAAAGCCTCTGTGCCGGT
59.89
CACCCGAAGCCTCATCAACT
60.04
153
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TTTAAAGCCTCTGTGCCGGT
59.89
TCACCCGAAGCCTCATCAAC
60.04
154
PCR template sequence:
GACGTTTTAAAGCCTCTGTGCCGGTCTCCCCAAAGTGTTGAGCCAAAGTACAAACCGCTTGGAAAATTGAAGAGAGCCAGAACAATCCATCTTGACTCGGATAACAATTGAAATTGAAACTCCTACGCAGATATCTTTAGTTGATGAGGCTTCGGGTGAGAAGGAAGAGGTTGTTGTGACTCTCTTACCAGCTGGTCACT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
6
Interacting miRNAs (#binding sites)
mmu-miR-34c-5p(1), mmu-miR-34a-5p(1), mmu-miR-181b-5p(1), mmu-miR-149-5p(1), mmu-miR-34b-5p(1), mmu-miR-181d-5p(1)
siRNAs for circular RNA silencing
Junction sequence
CATCTTGACTCGGATAACAATTGAAA
siRNA sets
AATTGTTATCCGAGTCAAG, ATTGTTATCCGAGTCAAGA, TTTCAATTGTTATCCGAGT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCDCLRE1C_910_ORF_1
0.824
0.079082755
0.916337915
807
ORF sequence:
ATGTTTTTATTTCAGGGCAGTAATGGAACTGTCTTATACACAGGAGACTTCAGACTGGCAAAAGGAGAAGCTTCCAGAATGGAGCTTCTGCACTCTGGAGGCAGAGTAAAAGACATCCAAAGTGTGTATTTAGACACGACTTTCTGTGACCCAAGGTTTTATCAGATCCCAAGTCGTGAGCAGTGTTTGAGGGGCATTTTGGAGCTGGTTCGGAGCTGGGTCACTAGGAGTCCGCACCACGTCGTGTGGCTGAACTGTAAAGCAGCTTATGGCTACGAGTATTTATTCACCAACCTAAGCGAGGAGCTGGGAGTTCAGGTTCATGTGGACAAGCTGGACATGTTTAAAAACATGCCTGATATCCTGCACCATCTCACAACGGACAGAAACACCCAGATCCACGCCTGCCGCCACCCAAAGGCAGAAGAGTGTTTTCAGTGGAATAAACTACCCTGTGGTATAACTTCCCAAAACAAAACTGCACTCCACACAATCAGCATCAAGCCATCTACCATGTGGTTTGGAGAGAGGACCAGAAAAACCAACGTGATCGTTAGGACTGGAGAGAGCTCATACAGAGCTTGCTTCTCTTTTCACTCCTCCTTCAGTGAGATTAAAGATTTTTTGAGCTACATCTGCCCAGTGAATGTGTATCCAAATGTCATCCCAGTTGGCCTCACTGTGGATAAGGTCATGGACGTTTTAAAGCCTCTGTGCCGGTCTCCCCAAAGTGTTGAGCCAAAGTACAAACCGCTTGGAAAATTGAAGAGAGCCAGAACAATCCATCTTGACTCGGATAACAATTGA
Peptide sequence:
MFLFQGSNGTVLYTGDFRLAKGEASRMELLHSGGRVKDIQSVYLDTTFCDPRFYQIPSREQCLRGILELVRSWVTRSPHHVVWLNCKAAYGYEYLFTNLSEELGVQVHVDKLDMFKNMPDILHHLTTDRNTQIHACRHPKAEECFQWNKLPCGITSQNKTALHTISIKPSTMWFGERTRKTNVIVRTGESSYRACFSFHSSFSEIKDFLSYICPVNVYPNVIPVGLTVDKVMDVLKPLCRSPQSVEPKYKPLGKLKRARTIHLDSDNN
IRESfinder result
IRESfinder score
0.730638595
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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