top of page
mmu_circDcaf7_447
Basic information of circular RNA
PanCircBase ID:
mmu_circDcaf7_447
mm39_Chr. Position ID:
chr11|105938968|105944674|+
Gene name:
Dcaf7
Transcript:
ENSMUST00000058438.9
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
4,5,6
Exon/intron offsets:
0,3614,5588
Genomic length:
5706
Exon/intron sizes in nt:
11,92,10,118
Flanking exon/introns:
chr11:105938574-105938968|chr11:105944674-105945508
Splice length:
447
Other database IDs:
CIRCpedia: MMU_CIRCpedia_1670
Human Ortholog IDs:
Mature spliced Sequence:
GTACCTCAAGCATTGACACAACGTGCACCATCTGGGGACTAGAGACCGGGCAAGTGCTGGGACGAGTGAATCTCGTGTCTGGCCATGTGAAGACCCAGCTAATTGCCCATGACAAAGAGGTCTATGACATCGCCTTCAGCCGCGCTGGAGGGGGCAGGGACATGTTTGCCTCTGTGGGCGCTGATGGGTCTGTGAGAATGTTTGACCTCCGCCATCTGGAACATAGCACCATCATTTATGAAGACCCACAGCACCACCCCCTGCTTCGCCTCTGCTGGAACAAGCAGGACCCTAACTACCTGGCCACCATGGCCATGGACGGGATGGAGGTGGTGATTCTGGATGTTCGAGTTCCCTGCACACCGGTTGCTAGGCTAAACAACCACCGAGCATGTGTCAATGGCATTGCCTGGGCCCCACACTCGTCCTGCCACATCTGTACTGCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
ACCACCGAGCATGTGTCAAT
59.96
GGTCTTCACATGGCCAGACA
59.96
161
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
ACCACCGAGCATGTGTCAAT
59.96
TGGCCAGACACGAGATTCAC
60.04
151
PCR template sequence:
CGAGTTCCCTGCACACCGGTTGCTAGGCTAAACAACCACCGAGCATGTGTCAATGGCATTGCCTGGGCCCCACACTCGTCCTGCCACATCTGTACTGCAGGTACCTCAAGCATTGACACAACGTGCACCATCTGGGGACTAGAGACCGGGCAAGTGCTGGGACGAGTGAATCTCGTGTCTGGCCATGTGAAGACCCAGCT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
1
Interacting miRNAs (#binding sites)
mmu-miR-196a-5p(1)
siRNAs for circular RNA silencing
Junction sequence
ATCTGTACTGCAGGTACCTCAAGCAT
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCDCAF7_447_ORF_1
1.1839
0.313347718
0.9707197
732
ORF sequence:
ATGTTTGCCTCTGTGGGCGCTGATGGGTCTGTGAGAATGTTTGACCTCCGCCATCTGGAACATAGCACCATCATTTATGAAGACCCACAGCACCACCCCCTGCTTCGCCTCTGCTGGAACAAGCAGGACCCTAACTACCTGGCCACCATGGCCATGGACGGGATGGAGGTGGTGATTCTGGATGTTCGAGTTCCCTGCACACCGGTTGCTAGGCTAAACAACCACCGAGCATGTGTCAATGGCATTGCCTGGGCCCCACACTCGTCCTGCCACATCTGTACTGCAGGTACCTCAAGCATTGACACAACGTGCACCATCTGGGGACTAGAGACCGGGCAAGTGCTGGGACGAGTGAATCTCGTGTCTGGCCATGTGAAGACCCAGCTAATTGCCCATGACAAAGAGGTCTATGACATCGCCTTCAGCCGCGCTGGAGGGGGCAGGGACATGTTTGCCTCTGTGGGCGCTGATGGGTCTGTGAGAATGTTTGACCTCCGCCATCTGGAACATAGCACCATCATTTATGAAGACCCACAGCACCACCCCCTGCTTCGCCTCTGCTGGAACAAGCAGGACCCTAACTACCTGGCCACCATGGCCATGGACGGGATGGAGGTGGTGATTCTGGATGTTCGAGTTCCCTGCACACCGGTTGCTAGGCTAAACAACCACCGAGCATGTGTCAATGGCATTGCCTGGGCCCCACACTCGTCCTGCCACATCTGTACTGCA
Peptide sequence:
MFASVGADGSVRMFDLRHLEHSTIIYEDPQHHPLLRLCWNKQDPNYLATMAMDGMEVVILDVRVPCTPVARLNNHRACVNGIAWAPHSSCHICTAGTSSIDTTCTIWGLETGQVLGRVNLVSGHVKTQLIAHDKEVYDIAFSRAGGGRDMFASVGADGSVRMFDLRHLEHSTIIYEDPQHHPLLRLCWNKQDPNYLATMAMDGMEVVILDVRVPCTPVARLNNHRACVNGIAWAPHSSCHICTA
IRESfinder result
IRESfinder score
0.442710916
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
bottom of page