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mmu_circDcaf6_429
Basic information of circular RNA
PanCircBase ID:
mmu_circDcaf6_429
mm39_Chr. Position ID:
chr1|165217982|165227437|-
Gene name:
Dcaf6
Transcript:
ENSMUST00000027856.13
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
9,8,7
Exon/intron offsets:
0,1725,9240
Genomic length:
9455
Exon/intron sizes in nt:
1,20,94,215
Flanking exon/introns:
chr1:165216484-165217982|chr1:165227437-165239032
Splice length:
429
Other database IDs:
circAtlas: mmu-Dcaf6_0032
Human Ortholog IDs:
Mature spliced Sequence:
GGAATTATGCAGGCCGAGGAACTACTGGAATGGTTGCTCGATTTATACCTTCTCATCTTAGTAACAAATCATGCAGAGTGACATCACTGTGTTACAGTGAAGATGGTCAAGAGATTCTTGTCAGTTATTCTTCAGACTACATCTATCTTTTTGACCCCAAAGATGATACTGCACGAGAACTTAAAACTCCTTCTGCAGAGGAGAGGCGAGAAGAGTTACGACAGCCTCCAGTTAAGCGCTTGAGACTTCGTGGTGATTGGTCAGATACTGGTCCCAGAGCACGGCCAGAAAGTGAACGAGAACGAGATGGAGAGCAAAGTCCCAATGTGTCACTGATGCAGAGAATGTCTGATATGTTATCAAGGTGGTTTGAAGAAGCAAGTGAAGTTGCACAAAGCAACAGAGGAAGAGGAAGACCTCGGCCCAGAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TCACTGATGCAGAGAATGTCT
56.74
TCGAGCAACCATTCCAGTAGT
59.1
141
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TCACTGATGCAGAGAATGTCT
56.74
TCGAGCAACCATTCCAGTAGTT
59.7
141
PCR template sequence:
TCACTGATGCAGAGAATGTCTGATATGTTATCAAGGTGGTTTGAAGAAGCAAGTGAAGTTGCACAAAGCAACAGAGGAAGAGGAAGACCTCGGCCCAGAGGGAATTATGCAGGCCGAGGAACTACTGGAATGGTTGCTCGATTTATACCTTCTCATCTTAGTAACAAATCATGCAGAGTGACATCACTGTGTTACAGTGA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
1
Interacting miRNAs (#binding sites)
mmu-miR-326-3p(1)
siRNAs for circular RNA silencing
Junction sequence
CCTCGGCCCAGAGGGAATTATGCAGG
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCDCAF6_429_ORF_1
1.1616
0.08765497
0.961966514
828
ORF sequence:
ATGGTTGCTCGATTTATACCTTCTCATCTTAGTAACAAATCATGCAGAGTGACATCACTGTGTTACAGTGAAGATGGTCAAGAGATTCTTGTCAGTTATTCTTCAGACTACATCTATCTTTTTGACCCCAAAGATGATACTGCACGAGAACTTAAAACTCCTTCTGCAGAGGAGAGGCGAGAAGAGTTACGACAGCCTCCAGTTAAGCGCTTGAGACTTCGTGGTGATTGGTCAGATACTGGTCCCAGAGCACGGCCAGAAAGTGAACGAGAACGAGATGGAGAGCAAAGTCCCAATGTGTCACTGATGCAGAGAATGTCTGATATGTTATCAAGGTGGTTTGAAGAAGCAAGTGAAGTTGCACAAAGCAACAGAGGAAGAGGAAGACCTCGGCCCAGAGGGAATTATGCAGGCCGAGGAACTACTGGAATGGTTGCTCGATTTATACCTTCTCATCTTAGTAACAAATCATGCAGAGTGACATCACTGTGTTACAGTGAAGATGGTCAAGAGATTCTTGTCAGTTATTCTTCAGACTACATCTATCTTTTTGACCCCAAAGATGATACTGCACGAGAACTTAAAACTCCTTCTGCAGAGGAGAGGCGAGAAGAGTTACGACAGCCTCCAGTTAAGCGCTTGAGACTTCGTGGTGATTGGTCAGATACTGGTCCCAGAGCACGGCCAGAAAGTGAACGAGAACGAGATGGAGAGCAAAGTCCCAATGTGTCACTGATGCAGAGAATGTCTGATATGTTATCAAGGTGGTTTGAAGAAGCAAGTGAAGTTGCACAAAGCAACAGAGGAAGAGGAAGACCTCGGCCCAGA
Peptide sequence:
MVARFIPSHLSNKSCRVTSLCYSEDGQEILVSYSSDYIYLFDPKDDTARELKTPSAEERREELRQPPVKRLRLRGDWSDTGPRARPESERERDGEQSPNVSLMQRMSDMLSRWFEEASEVAQSNRGRGRPRPRGNYAGRGTTGMVARFIPSHLSNKSCRVTSLCYSEDGQEILVSYSSDYIYLFDPKDDTARELKTPSAEERREELRQPPVKRLRLRGDWSDTGPRARPESERERDGEQSPNVSLMQRMSDMLSRWFEEASEVAQSNRGRGRPRPR
IRESfinder result
IRESfinder score
0.5491316
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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