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mmu_circDcaf6_1063
Basic information of circular RNA
PanCircBase ID:
mmu_circDcaf6_1063
mm39_Chr. Position ID:
chr1|165198691|165247793|-
Gene name:
Dcaf6
Transcript:
ENSMUST00000195686.2
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
7
Exon/intron indexes:
11,10,9,8,7,6,5
Exon/intron offsets:
0,17535,19291,21016,28531,40341,48988
Genomic length:
49102
Exon/intron sizes in nt:
1,26,25,81,20,94,21,51,00,000
Flanking exon/introns:
chr1:165195074-165198691|chr1:165247793-165250424
Splice length:
1063
Other database IDs:
circAtlas: hsa-SCAF8_0001
Human Ortholog IDs:
Mature spliced Sequence:
ATTATGACTGTACCAAACGACCCTTACACCTTTCTGTCCTGTGGTGAAGATGGAACTGTTAGGTGGTTTGACACACGCATCAAAACCAGTTGCACAAAAGAAGACTGTAAAGATGATATTTTAATCAACTGTAGGCGTGCTGCCACATCTGTGGCTATTTGTCCCCCAGTACCATATTACCTTGCTGTGGGTTGTTCTGACAGCTCAGTACGGATTTATGATCGGCGAATGCTGGGCACAAGAGCTACAGGGAATTATGCAGGCCGAGGAACTACTGGAATGGTTGCTCGATTTATACCTTCTCATCTTAGTAACAAATCATGCAGAGTGACATCACTGTGTTACAGTGAAGATGGTCAAGAGATTCTTGTCAGTTATTCTTCAGACTACATCTATCTTTTTGACCCCAAAGATGATACTGCACGAGAACTTAAAACTCCTTCTGCAGAGGAGAGGCGAGAAGAGTTACGACAGCCTCCAGTTAAGCGCTTGAGACTTCGTGGTGATTGGTCAGATACTGGTCCCAGAGCACGGCCAGAAAGTGAACGAGAACGAGATGGAGAGCAAAGTCCCAATGTGTCACTGATGCAGAGAATGTCTGATATGTTATCAAGGTGGTTTGAAGAAGCAAGTGAAGTTGCACAAAGCAACAGAGGAAGAGGAAGACCTCGGCCCAGAGGTGGAACAAATCAGCCAGATGTTTCAACTCTTCCTACGGTTCCATCAAGTCCTAATTTGGAAGTGTGTGAAACTGCAATGGATGTAGACATGCCAGCTGCACTTCTTCAGCCTTCTACATCCTCTACAGATCCAGTTCAGGCTCAGGCAGCCACAGCCGCCATAGAAAGCCCTCGTTCCAGCTCGTTGCTGTCTTGCCCAGACAGTGAACCGAGGCAGTCTGTTGAGGCGTCTGGACACCATGCACATCATCAGTCAGGTCCCCTGAAGTCATGCATGGGTTGCTGGCTCCTGCTCAGAGTGACAACACCCTGTGGAATCTACTGGTACTTGCCCAACCACCACAGAGCCCACTGGAGCATGGAGCCAGCCTAGAGAGGGTCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TGCTCAGAGTGACAACACCC
59.89
GATGCGTGTGTCAAACCACC
60.04
174
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
ACTGGTACTTGCCCAACCAC
60.18
GATGCGTGTGTCAAACCACC
60.04
144
PCR template sequence:
TGGCTCCTGCTCAGAGTGACAACACCCTGTGGAATCTACTGGTACTTGCCCAACCACCACAGAGCCCACTGGAGCATGGAGCCAGCCTAGAGAGGGTCAGATTATGACTGTACCAAACGACCCTTACACCTTTCTGTCCTGTGGTGAAGATGGAACTGTTAGGTGGTTTGACACACGCATCAAAACCAGTTGCACAAAAG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
7
Interacting miRNAs (#binding sites)
mmu-miR-31-5p(1), mmu-miR-184-3p(1), mmu-miR-326-3p(1), mmu-miR-181b-5p(1), mmu-miR-149-5p(1), mmu-miR-377-3p(1), mmu-miR-338-3p(1)
siRNAs for circular RNA silencing
Junction sequence
TAGAGAGGGTCAGATTATGACTGTAC
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCDCAF6_1063_ORF_5
0.9194
0.02303019
0.067677665
120
ORF sequence:
ATGCATGGGTTGCTGGCTCCTGCTCAGAGTGACAACACCCTGTGGAATCTACTGGTACTTGCCCAACCACCACAGAGCCCACTGGAGCATGGAGCCAGCCTAGAGAGGGTCAGATTATGA
Peptide sequence:
MHGLLAPAQSDNTLWNLLVLAQPPQSPLEHGASLERVRL
IRESfinder result
IRESfinder score
0.540454871
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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