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mmu_circDazl_291
Basic information of circular RNA
PanCircBase ID:
mmu_circDazl_291
mm39_Chr. Position ID:
chr17|50595138|50596266|-
Gene name:
Dazl
Transcript:
ENSMUST00000233248.2
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
4,3,2
Exon/intron offsets:
0,503,981
Genomic length:
1128
Exon/intron sizes in nt:
52,92,147
Flanking exon/introns:
chr17:50594846-50595138|chr17:50596266-50599848
Splice length:
291
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
TCTGCCACAACTTCTGAGGCTCCAAATTCAGCTGTCTCCAGGGAGGCCAGCACTCAGTCTTCATCAGCAACCACAAGTCAAGGATATGTTTTGCCAGAAGGCAAAATCATGCCAAACACCGTTTTTGTTGGAGGAATTGATGTTAGGATGGATGAAACCGAAATCAGGAGTTTCTTTGCCAGATATGGCTCAGTAAAAGAAGTGAAGATAATCACTGATCGAACTGGTGTGTCGAAGGGCTATGGATTTGTCTCATTTTATAATGACGTGGATGTGCAGAAGATAGTAGAA
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TGATCGAACTGGTGTGTCGA
59.04
ATGAAGACTGAGTGCTGGCC
60.04
140
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CTGATCGAACTGGTGTGTCGA
60.07
ATGAAGACTGAGTGCTGGCC
60.04
141
PCR template sequence:
AGTAAAAGAAGTGAAGATAATCACTGATCGAACTGGTGTGTCGAAGGGCTATGGATTTGTCTCATTTTATAATGACGTGGATGTGCAGAAGATAGTAGAATCTGCCACAACTTCTGAGGCTCCAAATTCAGCTGTCTCCAGGGAGGCCAGCACTCAGTCTTCATCAGCAACCACAAGTCAAGGATATGTTTTGCCAGAAG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
1
Interacting miRNAs (#binding sites)
mmu-miR-3065-5p(1)
siRNAs for circular RNA silencing
Junction sequence
GAAGATAGTAGAATCTGCCACAACTT
siRNA sets
AGTTGTGGCAGATTCTACT, TGTGGCAGATTCTACTATC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCDAZL_291_ORF_1
0.9499
0.134382991
0.617682382
474
ORF sequence:
ATGCCAAACACCGTTTTTGTTGGAGGAATTGATGTTAGGATGGATGAAACCGAAATCAGGAGTTTCTTTGCCAGATATGGCTCAGTAAAAGAAGTGAAGATAATCACTGATCGAACTGGTGTGTCGAAGGGCTATGGATTTGTCTCATTTTATAATGACGTGGATGTGCAGAAGATAGTAGAATCTGCCACAACTTCTGAGGCTCCAAATTCAGCTGTCTCCAGGGAGGCCAGCACTCAGTCTTCATCAGCAACCACAAGTCAAGGATATGTTTTGCCAGAAGGCAAAATCATGCCAAACACCGTTTTTGTTGGAGGAATTGATGTTAGGATGGATGAAACCGAAATCAGGAGTTTCTTTGCCAGATATGGCTCAGTAAAAGAAGTGAAGATAATCACTGATCGAACTGGTGTGTCGAAGGGCTATGGATTTGTCTCATTTTATAATGACGTGGATGTGCAGAAGATAGTAGAA
Peptide sequence:
MPNTVFVGGIDVRMDETEIRSFFARYGSVKEVKIITDRTGVSKGYGFVSFYNDVDVQKIVESATTSEAPNSAVSREASTQSSSATTSQGYVLPEGKIMPNTVFVGGIDVRMDETEIRSFFARYGSVKEVKIITDRTGVSKGYGFVSFYNDVDVQKIVE
IRESfinder result
IRESfinder score
0.392982442
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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