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mmu_circDars_838
Basic information of circular RNA
PanCircBase ID:
mmu_circDars_838
mm39_Chr. Position ID:
chr1|128294657|128314913|-
Gene name:
Dars
Transcript:
ENSMUST00000027602.15
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
8
Exon/intron indexes:
14,13,12,11,10,9,8,7
Exon/intron offsets:
0,1444,2572,5219,7016,9257,11845,20196
Genomic length:
20256
Exon/intron sizes in nt:
112,81,43,147,148,135,112,60
Flanking exon/introns:
chr1:128294506-128294657|chr1:128314913-128316855
Splice length:
838
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GACGGAAGAGCTACGGTTAACCAGGATACAAGATTAGACAACAGAGTCATTGACCTTAGGACATCAACTAGTCAGGCCATCTTCCGTCTCCAGTCCGGCATCTGCCATCTCTTCCGAGAAACTTTAATTAACAAAGGTTTTGTGGAAATCCAAACTCCTAAAATAATTTCAGCTGCCAGTGAAGGAGGAGCCAATGTTTTTACTGTGTCCTATTTTAAAAATAATGCCTACCTTGCTCAGTCTCCACAATTGTATAAGCAGATGTGCATTTGTGCTGATTTTGAGAAGGTTTTCTGCATTGGACCAGTTTTCAGAGCTGAAGACTCCAATACCCATAGACATCTGACTGAATTTGTTGGTTTGGATATTGAAATGGCCTTTAATTATCATTACCATGAAGTTGTAGAAGAGATTGCTGACACTTTGGTGCAAATATTCAAAGGACTTCAAGAAAGGTTTCAGACTGAAATTCAAACTGTGAGTAAACAGTTCCCATGTGAGCCGTTCAAATTTTTGGAGCCAACTTTAAGACTGGAGTATTGCGAGGCTTTGGCAATGCTTAGAGAAGCTGGAGTTGAAATGGACGATGAGGAAGATCTAAGTACACCAAATGAAAAACTGTTGGGTCGTTTGGTGAAGGAAAAGTATGATACAGACTTTTATGTTCTTGATAAATATCCATTGGCTGTAAGACCTTTCTATACCATGCCTGACCCAAGAAATCCGAAACAGTCCAACTCTTACGATATGTTCATGAGAGGAGAAGAAATATTGTCTGGAGCACAAAGAATCCATGATCCTCAGTTGCTTACAGAGAGAGCTTTACACCATGGAATTG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TGTCTGGAGCACAAAGAATCCA
59.89
CGGACTGGAGACGGAAGATG
59.9
163
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TGTCTGGAGCACAAAGAATCCA
59.89
GAGACGGAAGATGGCCTGAC
60.18
156
PCR template sequence:
TCTTACGATATGTTCATGAGAGGAGAAGAAATATTGTCTGGAGCACAAAGAATCCATGATCCTCAGTTGCTTACAGAGAGAGCTTTACACCATGGAATTGGACGGAAGAGCTACGGTTAACCAGGATACAAGATTAGACAACAGAGTCATTGACCTTAGGACATCAACTAGTCAGGCCATCTTCCGTCTCCAGTCCGGCA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
4
Interacting miRNAs (#binding sites)
mmu-miR-452-3p(1), mmu-miR-3065-5p(1), mmu-miR-3105-3p(1), mmu-miR-7a-1-3p(1)
siRNAs for circular RNA silencing
Junction sequence
CACCATGGAATTGGACGGAAGAGCTA
siRNA sets
AGCTCTTCCGTCCAATTCC, TAGCTCTTCCGTCCAATTC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCDARS_838_ORF_1
0.8574
0.0384448
0.944493835
882
ORF sequence:
ATGATCCTCAGTTGCTTACAGAGAGAGCTTTACACCATGGAATTGGACGGAAGAGCTACGGTTAACCAGGATACAAGATTAGACAACAGAGTCATTGACCTTAGGACATCAACTAGTCAGGCCATCTTCCGTCTCCAGTCCGGCATCTGCCATCTCTTCCGAGAAACTTTAATTAACAAAGGTTTTGTGGAAATCCAAACTCCTAAAATAATTTCAGCTGCCAGTGAAGGAGGAGCCAATGTTTTTACTGTGTCCTATTTTAAAAATAATGCCTACCTTGCTCAGTCTCCACAATTGTATAAGCAGATGTGCATTTGTGCTGATTTTGAGAAGGTTTTCTGCATTGGACCAGTTTTCAGAGCTGAAGACTCCAATACCCATAGACATCTGACTGAATTTGTTGGTTTGGATATTGAAATGGCCTTTAATTATCATTACCATGAAGTTGTAGAAGAGATTGCTGACACTTTGGTGCAAATATTCAAAGGACTTCAAGAAAGGTTTCAGACTGAAATTCAAACTGTGAGTAAACAGTTCCCATGTGAGCCGTTCAAATTTTTGGAGCCAACTTTAAGACTGGAGTATTGCGAGGCTTTGGCAATGCTTAGAGAAGCTGGAGTTGAAATGGACGATGAGGAAGATCTAAGTACACCAAATGAAAAACTGTTGGGTCGTTTGGTGAAGGAAAAGTATGATACAGACTTTTATGTTCTTGATAAATATCCATTGGCTGTAAGACCTTTCTATACCATGCCTGACCCAAGAAATCCGAAACAGTCCAACTCTTACGATATGTTCATGAGAGGAGAAGAAATATTGTCTGGAGCACAAAGAATCCATGATCCTCAGTTGCTTACAGAGAGAGCTTTACACCATGGAATT
Peptide sequence:
MILSCLQRELYTMELDGRATVNQDTRLDNRVIDLRTSTSQAIFRLQSGICHLFRETLINKGFVEIQTPKIISAASEGGANVFTVSYFKNNAYLAQSPQLYKQMCICADFEKVFCIGPVFRAEDSNTHRHLTEFVGLDIEMAFNYHYHEVVEEIADTLVQIFKGLQERFQTEIQTVSKQFPCEPFKFLEPTLRLEYCEALAMLREAGVEMDDEEDLSTPNEKLLGRLVKEKYDTDFYVLDKYPLAVRPFYTMPDPRNPKQSNSYDMFMRGEEILSGAQRIHDPQLLTERALHHGI
IRESfinder result
IRESfinder score
0.707735962
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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