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mmu_circDars_786
Basic information of circular RNA
PanCircBase ID:
mmu_circDars_786
mm39_Chr. Position ID:
chr1|128299876|128319110|-
Gene name:
Dars
Transcript:
ENSMUST00000027602.15
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
7
Exon/intron indexes:
11,10,9,8,7,6,5
Exon/intron offsets:
0,1797,4038,6626,14977,16979,19131
Genomic length:
19234
Exon/intron sizes in nt:
14,71,48,13,51,12,60,80,000
Flanking exon/introns:
chr1:128297272-128299876|chr1:128319110-128333102
Splice length:
786
Other database IDs:
CIRCpedia: MMU_CIRCpedia_7167
Human Ortholog IDs:
Mature spliced Sequence:
CATCAACAAAGAAAGTATTATTGACGTAGAAGGTGTTGTGAGGAAAGTCAATCAGAAAATTGGAAGCTGTACCCAGCAGGATGTTGAGTTGCATGTCCAGAAGATTTATGTGATCAGCTTGGCTGAACCTCGCCTGCCCTTGCAGCTGGATGATGCCATTCGGCCTGAAGTGGAAGGAGAAGAGGACGGAAGAGCTACGGTTAACCAGGATACAAGATTAGACAACAGAGTCATTGACCTTAGGACATCAACTAGTCAGGCCATCTTCCGTCTCCAGTCCGGCATCTGCCATCTCTTCCGAGAAACTTTAATTAACAAAGGTTTTGTGGAAATCCAAACTCCTAAAATAATTTCAGCTGCCAGTGAAGGAGGAGCCAATGTTTTTACTGTGTCCTATTTTAAAAATAATGCCTACCTTGCTCAGTCTCCACAATTGTATAAGCAGATGTGCATTTGTGCTGATTTTGAGAAGGTTTTCTGCATTGGACCAGTTTTCAGAGCTGAAGACTCCAATACCCATAGACATCTGACTGAATTTGTTGGTTTGGATATTGAAATGGCCTTTAATTATCATTACCATGAAGTTGTAGAAGAGATTGCTGACACTTTGGTGCAAATATTCAAAGGACTTCAAGAAAGGTTTCAGACTGAAATTCAAACTGTGAGTAAACAGTTCCCATGTGAGCCGTTCAAATTTTTGGAGCCAACTTTAAGACTGGAGTATTGCGAGGCTTTGGCAATGCTTAGAGAAGCTGGAGTTGAAATGGACGATGAGGAAGATCTAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AGTATTGCGAGGCTTTGGCA
60.32
GCAACTCAACATCCTGCTGG
59.48
159
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GAGTATTGCGAGGCTTTGGC
59.62
GCAACTCAACATCCTGCTGG
59.48
160
PCR template sequence:
CGTTCAAATTTTTGGAGCCAACTTTAAGACTGGAGTATTGCGAGGCTTTGGCAATGCTTAGAGAAGCTGGAGTTGAAATGGACGATGAGGAAGATCTAAGCATCAACAAAGAAAGTATTATTGACGTAGAAGGTGTTGTGAGGAAAGTCAATCAGAAAATTGGAAGCTGTACCCAGCAGGATGTTGAGTTGCATGTCCAG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
6
Interacting miRNAs (#binding sites)
mmu-miR-370-3p(1), mmu-miR-207(1), mmu-miR-452-3p(1), mmu-miR-3065-5p(1), mmu-miR-3105-3p(1), mmu-miR-7a-1-3p(1)
siRNAs for circular RNA silencing
Junction sequence
AGGAAGATCTAAGCATCAACAAAGAA
siRNA sets
TTGATGCTTAGATCTTCCT, GTTGATGCTTAGATCTTCC, TTTGTTGATGCTTAGATCT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCDARS_786_ORF_1
1.0242
0.100992212
0.9942688
1125
ORF sequence:
ATGTGCATTTGTGCTGATTTTGAGAAGGTTTTCTGCATTGGACCAGTTTTCAGAGCTGAAGACTCCAATACCCATAGACATCTGACTGAATTTGTTGGTTTGGATATTGAAATGGCCTTTAATTATCATTACCATGAAGTTGTAGAAGAGATTGCTGACACTTTGGTGCAAATATTCAAAGGACTTCAAGAAAGGTTTCAGACTGAAATTCAAACTGTGAGTAAACAGTTCCCATGTGAGCCGTTCAAATTTTTGGAGCCAACTTTAAGACTGGAGTATTGCGAGGCTTTGGCAATGCTTAGAGAAGCTGGAGTTGAAATGGACGATGAGGAAGATCTAAGCATCAACAAAGAAAGTATTATTGACGTAGAAGGTGTTGTGAGGAAAGTCAATCAGAAAATTGGAAGCTGTACCCAGCAGGATGTTGAGTTGCATGTCCAGAAGATTTATGTGATCAGCTTGGCTGAACCTCGCCTGCCCTTGCAGCTGGATGATGCCATTCGGCCTGAAGTGGAAGGAGAAGAGGACGGAAGAGCTACGGTTAACCAGGATACAAGATTAGACAACAGAGTCATTGACCTTAGGACATCAACTAGTCAGGCCATCTTCCGTCTCCAGTCCGGCATCTGCCATCTCTTCCGAGAAACTTTAATTAACAAAGGTTTTGTGGAAATCCAAACTCCTAAAATAATTTCAGCTGCCAGTGAAGGAGGAGCCAATGTTTTTACTGTGTCCTATTTTAAAAATAATGCCTACCTTGCTCAGTCTCCACAATTGTATAAGCAGATGTGCATTTGTGCTGATTTTGAGAAGGTTTTCTGCATTGGACCAGTTTTCAGAGCTGAAGACTCCAATACCCATAGACATCTGACTGAATTTGTTGGTTTGGATATTGAAATGGCCTTTAATTATCATTACCATGAAGTTGTAGAAGAGATTGCTGACACTTTGGTGCAAATATTCAAAGGACTTCAAGAAAGGTTTCAGACTGAAATTCAAACTGTGAGTAAACAGTTCCCATGTGAGCCGTTCAAATTTTTGGAGCCAACTTTAAGACTGGAGTATTGCGAGGCTTTGGCAATGCTTAGAGAAGCTGGAGTTGAAATGGACGATGAGGAAGATCTA
Peptide sequence:
MCICADFEKVFCIGPVFRAEDSNTHRHLTEFVGLDIEMAFNYHYHEVVEEIADTLVQIFKGLQERFQTEIQTVSKQFPCEPFKFLEPTLRLEYCEALAMLREAGVEMDDEEDLSINKESIIDVEGVVRKVNQKIGSCTQQDVELHVQKIYVISLAEPRLPLQLDDAIRPEVEGEEDGRATVNQDTRLDNRVIDLRTSTSQAIFRLQSGICHLFRETLINKGFVEIQTPKIISAASEGGANVFTVSYFKNNAYLAQSPQLYKQMCICADFEKVFCIGPVFRAEDSNTHRHLTEFVGLDIEMAFNYHYHEVVEEIADTLVQIFKGLQERFQTEIQTVSKQFPCEPFKFLEPTLRLEYCEALAMLREAGVEMDDEEDL
IRESfinder result
IRESfinder score
0.601665798
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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