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mmu_circDars2_324
Basic information of circular RNA
PanCircBase ID:
mmu_circDars2_324
mm39_Chr. Position ID:
chr1|160874346|160879008|-
Gene name:
Dars2
Transcript:
ENSMUST00000035430.4
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
13,12,11
Exon/intron offsets:
0,3161,4554
Genomic length:
4662
Exon/intron sizes in nt:
1,53,63,108
Flanking exon/introns:
chr1:160873046-160874346|chr1:160879008-160881510
Splice length:
324
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
ATTGTGGATGTCAGTGATGTGTTCAGAAACACAGAGCTCAGATTCCTTCAGGATGCACTGGCTAAGCCCCAAGGAACTGTCAAAGCAATATGTGTTCATGACGGAGCGAAATACTTAAGGAAGGAAGACATTGAGTTCATTAGGAAGTTTGCAGTTCACCATTTCAGTCAGGAAGTCCTACCTATATTCCTGAATGCCAAGAAGAACTGGAGTTCGCCATTTGCTAAGTTCATAATGGAGGAGGAAAGACTGGAATTAGCCAGATCGATGGAGATACAAGAAGAAGACATAGTGTTACTGACTGCTGGGGAACATGAGAAAGCA
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TGGAGGAGGAAAGACTGGAA
57.58
ACAGTTCCTTGGGGCTTAGC
59.96
169
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TGGAGGAGGAAAGACTGGAA
57.58
GCTTAGCCAGTGCATCCTGA
60.11
156
PCR template sequence:
TAAGTTCATAATGGAGGAGGAAAGACTGGAATTAGCCAGATCGATGGAGATACAAGAAGAAGACATAGTGTTACTGACTGCTGGGGAACATGAGAAAGCAATTGTGGATGTCAGTGATGTGTTCAGAAACACAGAGCTCAGATTCCTTCAGGATGCACTGGCTAAGCCCCAAGGAACTGTCAAAGCAATATGTGTTCATG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
6
Interacting miRNAs (#binding sites)
mmu-miR-152-3p(1), mmu-miR-148b-3p(1), mmu-miR-148a-3p(1), mmu-miR-182-5p(1), mmu-miR-27a-5p(1), mmu-miR-802-5p(1)
siRNAs for circular RNA silencing
Junction sequence
ACATGAGAAAGCAATTGTGGATGTCA
siRNA sets
ATCCACAATTGCTTTCTCA, CATCCACAATTGCTTTCTC, ACATCCACAATTGCTTTCT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCDARS2_324_ORF_1
0.9809
0.163108029
0.554852117
414
ORF sequence:
ATGGAGGAGGAAAGACTGGAATTAGCCAGATCGATGGAGATACAAGAAGAAGACATAGTGTTACTGACTGCTGGGGAACATGAGAAAGCAATTGTGGATGTCAGTGATGTGTTCAGAAACACAGAGCTCAGATTCCTTCAGGATGCACTGGCTAAGCCCCAAGGAACTGTCAAAGCAATATGTGTTCATGACGGAGCGAAATACTTAAGGAAGGAAGACATTGAGTTCATTAGGAAGTTTGCAGTTCACCATTTCAGTCAGGAAGTCCTACCTATATTCCTGAATGCCAAGAAGAACTGGAGTTCGCCATTTGCTAAGTTCATAATGGAGGAGGAAAGACTGGAATTAGCCAGATCGATGGAGATACAAGAAGAAGACATAGTGTTACTGACTGCTGGGGAACATGAGAAAGCA
Peptide sequence:
MEEERLELARSMEIQEEDIVLLTAGEHEKAIVDVSDVFRNTELRFLQDALAKPQGTVKAICVHDGAKYLRKEDIEFIRKFAVHHFSQEVLPIFLNAKKNWSSPFAKFIMEEERLELARSMEIQEEDIVLLTAGEHEKA
IRESfinder result
IRESfinder score
0.434670657
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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