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mmu_circDaglb_891
Basic information of circular RNA
PanCircBase ID:
mmu_circDaglb_891
mm39_Chr. Position ID:
chr5|143464168|143486952|+
Gene name:
Daglb
Transcript:
ENSMUST00000045593.12
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
9
Exon/intron indexes:
5,6,7,8,9,10,11,12,13
Exon/intron offsets:
0,3216,5812,8734,11513,15744,16128,22556,22711
Genomic length:
22784
Exon/intron sizes in nt:
123,128,127,84,78,81,128,69,73
Flanking exon/introns:
chr5:143460626-143464168|chr5:143486952-143488616
Splice length:
891
Other database IDs:
circAtlas: mmu-Daglb_0027
circAtlas: hsa-SCAF8_0001, CIRCpedia: HSA_CIRCpedia_58448
Human Ortholog IDs:
Mature spliced Sequence:
GACACAGACCTGGTGCCTAGTGATATCGCAGCAGGCTTCACCCTTCTCCACCAGCAACAAGACAATATCAGCCACAGCCGGGAGCCTCCGGAGGTCGTGACCCACACACCAGGACAGCCTCAGGAAACTGAGTTAGATGCAGAAGTGGAGAACTGCCATCATTACATGCCGTTTGCAGCAGCTGCCTATGGGTGGCCGCTCTACATCTACAGGAACCCGTTCACGGGGCTGTGCAGGATTGGTGGCGACTGTTGCAGAGCCAGAGACATAGAGTACGATGCAGTGGAAGGTGACCAGCACAACTGCCACTTCGCCTCCATCCTGAAGACCACAGGGCTGCAGTACAGGGATTTCATTCACATAAGCTTTCACGACAAGGTGTATGAGCTGCCCTTCATAGTGGTTCTGGACCACAGGAAGGAGTCTGTTGTGGTCGCCGTGAGAGGGACCATGTCTCTCCAGGACGTCCTGACCGACCTGTCTGCCGAGAGTGAGACCCTGGAGCTGGGGATTGAGCTACAGGACTGTGTGGCCCACAAGGGAATTGCTCAAGCGGCCAGATACATTCACCGCAGACTGGTCAATGATGGGATTCTGAGCCAAGCCTTCAGTGTCGCTCCGGAGTACCAGCTCGTTCTGGTGGGGCACAGCCTAGGAGCCGGCGCTGCGGCCCTGTTAGCCATCATGCTCCGGGGGGCCTACCCACAAGTCCGTGCTTATGCCTTCTCCCCGCCCAGGGGGCTGCTCAGCAAATCCCTTTACGAGTACTCCAAGGACTTTGTCGTGTCGCTTATCCTAGGGATGGATGTGATCCCCAGGTTAAGTGTGACCAACATGGAGGACCTCAAGAGGAGGATCCTGAGAGTGATCGCTAACTGCAATAAGCCGAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TCCCCAGGTTAAGTGTGACC
58.94
TTGTTGCTGGTGGAGAAGGG
60.18
140
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CCCCAGGTTAAGTGTGACCA
59.23
TGTCTTGTTGCTGGTGGAGA
59.16
143
PCR template sequence:
TATCCTAGGGATGGATGTGATCCCCAGGTTAAGTGTGACCAACATGGAGGACCTCAAGAGGAGGATCCTGAGAGTGATCGCTAACTGCAATAAGCCGAAGGACACAGACCTGGTGCCTAGTGATATCGCAGCAGGCTTCACCCTTCTCCACCAGCAACAAGACAATATCAGCCACAGCCGGGAGCCTCCGGAGGTCGTGA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
8
Interacting miRNAs (#binding sites)
mmu-miR-217-5p(1), mmu-miR-132-3p(1), mmu-miR-185-5p(1), mmu-miR-149-5p(1), mmu-miR-212-3p(1), mmu-miR-370-3p(1), mmu-miR-541-5p(1), mmu-miR-490-3p(1)
siRNAs for circular RNA silencing
Junction sequence
CAATAAGCCGAAGGACACAGACCTGG
siRNA sets
CAGGTCTGTGTCCTTCGGC, CCAGGTCTGTGTCCTTCGG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCDAGLB_891_ORF_1
1.2809
0.36101108
0.999964363
1617
ORF sequence:
ATGCCGTTTGCAGCAGCTGCCTATGGGTGGCCGCTCTACATCTACAGGAACCCGTTCACGGGGCTGTGCAGGATTGGTGGCGACTGTTGCAGAGCCAGAGACATAGAGTACGATGCAGTGGAAGGTGACCAGCACAACTGCCACTTCGCCTCCATCCTGAAGACCACAGGGCTGCAGTACAGGGATTTCATTCACATAAGCTTTCACGACAAGGTGTATGAGCTGCCCTTCATAGTGGTTCTGGACCACAGGAAGGAGTCTGTTGTGGTCGCCGTGAGAGGGACCATGTCTCTCCAGGACGTCCTGACCGACCTGTCTGCCGAGAGTGAGACCCTGGAGCTGGGGATTGAGCTACAGGACTGTGTGGCCCACAAGGGAATTGCTCAAGCGGCCAGATACATTCACCGCAGACTGGTCAATGATGGGATTCTGAGCCAAGCCTTCAGTGTCGCTCCGGAGTACCAGCTCGTTCTGGTGGGGCACAGCCTAGGAGCCGGCGCTGCGGCCCTGTTAGCCATCATGCTCCGGGGGGCCTACCCACAAGTCCGTGCTTATGCCTTCTCCCCGCCCAGGGGGCTGCTCAGCAAATCCCTTTACGAGTACTCCAAGGACTTTGTCGTGTCGCTTATCCTAGGGATGGATGTGATCCCCAGGTTAAGTGTGACCAACATGGAGGACCTCAAGAGGAGGATCCTGAGAGTGATCGCTAACTGCAATAAGCCGAAGGACACAGACCTGGTGCCTAGTGATATCGCAGCAGGCTTCACCCTTCTCCACCAGCAACAAGACAATATCAGCCACAGCCGGGAGCCTCCGGAGGTCGTGACCCACACACCAGGACAGCCTCAGGAAACTGAGTTAGATGCAGAAGTGGAGAACTGCCATCATTACATGCCGTTTGCAGCAGCTGCCTATGGGTGGCCGCTCTACATCTACAGGAACCCGTTCACGGGGCTGTGCAGGATTGGTGGCGACTGTTGCAGAGCCAGAGACATAGAGTACGATGCAGTGGAAGGTGACCAGCACAACTGCCACTTCGCCTCCATCCTGAAGACCACAGGGCTGCAGTACAGGGATTTCATTCACATAAGCTTTCACGACAAGGTGTATGAGCTGCCCTTCATAGTGGTTCTGGACCACAGGAAGGAGTCTGTTGTGGTCGCCGTGAGAGGGACCATGTCTCTCCAGGACGTCCTGACCGACCTGTCTGCCGAGAGTGAGACCCTGGAGCTGGGGATTGAGCTACAGGACTGTGTGGCCCACAAGGGAATTGCTCAAGCGGCCAGATACATTCACCGCAGACTGGTCAATGATGGGATTCTGAGCCAAGCCTTCAGTGTCGCTCCGGAGTACCAGCTCGTTCTGGTGGGGCACAGCCTAGGAGCCGGCGCTGCGGCCCTGTTAGCCATCATGCTCCGGGGGGCCTACCCACAAGTCCGTGCTTATGCCTTCTCCCCGCCCAGGGGGCTGCTCAGCAAATCCCTTTACGAGTACTCCAAGGACTTTGTCGTGTCGCTTATCCTAGGGATGGATGTGATCCCCAGGTTAAGTGTGACCAACATGGAGGACCTCAAGAGGAGGATCCTGAGAGTGATCGCTAACTGCAATAAGCCGAAG
Peptide sequence:
MPFAAAAYGWPLYIYRNPFTGLCRIGGDCCRARDIEYDAVEGDQHNCHFASILKTTGLQYRDFIHISFHDKVYELPFIVVLDHRKESVVVAVRGTMSLQDVLTDLSAESETLELGIELQDCVAHKGIAQAARYIHRRLVNDGILSQAFSVAPEYQLVLVGHSLGAGAAALLAIMLRGAYPQVRAYAFSPPRGLLSKSLYEYSKDFVVSLILGMDVIPRLSVTNMEDLKRRILRVIANCNKPKDTDLVPSDIAAGFTLLHQQQDNISHSREPPEVVTHTPGQPQETELDAEVENCHHYMPFAAAAYGWPLYIYRNPFTGLCRIGGDCCRARDIEYDAVEGDQHNCHFASILKTTGLQYRDFIHISFHDKVYELPFIVVLDHRKESVVVAVRGTMSLQDVLTDLSAESETLELGIELQDCVAHKGIAQAARYIHRRLVNDGILSQAFSVAPEYQLVLVGHSLGAGAAALLAIMLRGAYPQVRAYAFSPPRGLLSKSLYEYSKDFVVSLILGMDVIPRLSVTNMEDLKRRILRVIANCNKPK
IRESfinder result
IRESfinder score
0.42145813
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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