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mmu_circDaam1_324
Basic information of circular RNA
PanCircBase ID:
mmu_circDaam1_324
mm39_Chr. Position ID:
chr12|71993001|71993911|+
Gene name:
Daam1
Transcript:
ENSMUST00000223272.2
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
9,10,11
Exon/intron offsets:
0,287,771
Genomic length:
910
Exon/intron sizes in nt:
6,71,18,139
Flanking exon/introns:
chr12:71992275-71993001|chr12:71993911-71994166
Splice length:
324
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GCATTTAGACTTTTTTGAAATGCTCCGAAATGAAGATGAACTAGAATTTGCCAAAAGATTTGAACTGGTTCACATAGACACAAAAAGCGCCACTCAGATGTTTGAGCTGACCAGGAGGAGGCTAACCCACAGCGAAGCCTACCCCCACTTCATGTCCATTCTGCACCATTGCCTCCAGATGCCTTACAAGAGGAGTGGTAACACAGTTCAGTACTGGCTGCTGTTAGACAGAATCATACAGCAGATAGTTATCCAGAATGACAAAGGGCAGGATCCCGACTCCACACCTCTGGAAAACTTTAATATCAAGAACGTCGTAAGAAT
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
ATGACAAAGGGCAGGATCCC
59.74
TGAGTGGCGCTTTTTGTGTC
59.62
163
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
ATGACAAAGGGCAGGATCCC
59.74
CTGAGTGGCGCTTTTTGTGT
59.62
164
PCR template sequence:
TAGACAGAATCATACAGCAGATAGTTATCCAGAATGACAAAGGGCAGGATCCCGACTCCACACCTCTGGAAAACTTTAATATCAAGAACGTCGTAAGAATGCATTTAGACTTTTTTGAAATGCTCCGAAATGAAGATGAACTAGAATTTGCCAAAAGATTTGAACTGGTTCACATAGACACAAAAAGCGCCACTCAGATG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
3
Interacting miRNAs (#binding sites)
mmu-miR-211-5p(1), mmu-miR-204-5p(1), mmu-miR-680(1)
siRNAs for circular RNA silencing
Junction sequence
ACGTCGTAAGAATGCATTTAGACTTT
siRNA sets
TAAATGCATTCTTACGACG,AAAGTCTAAATGCATTCTT,AAATGCATTCTTACGACGT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCDAAM1_324_ORF_1
1.1999
0.221933515
0.917220214
627
ORF sequence:
ATGCTCCGAAATGAAGATGAACTAGAATTTGCCAAAAGATTTGAACTGGTTCACATAGACACAAAAAGCGCCACTCAGATGTTTGAGCTGACCAGGAGGAGGCTAACCCACAGCGAAGCCTACCCCCACTTCATGTCCATTCTGCACCATTGCCTCCAGATGCCTTACAAGAGGAGTGGTAACACAGTTCAGTACTGGCTGCTGTTAGACAGAATCATACAGCAGATAGTTATCCAGAATGACAAAGGGCAGGATCCCGACTCCACACCTCTGGAAAACTTTAATATCAAGAACGTCGTAAGAATGCATTTAGACTTTTTTGAAATGCTCCGAAATGAAGATGAACTAGAATTTGCCAAAAGATTTGAACTGGTTCACATAGACACAAAAAGCGCCACTCAGATGTTTGAGCTGACCAGGAGGAGGCTAACCCACAGCGAAGCCTACCCCCACTTCATGTCCATTCTGCACCATTGCCTCCAGATGCCTTACAAGAGGAGTGGTAACACAGTTCAGTACTGGCTGCTGTTAGACAGAATCATACAGCAGATAGTTATCCAGAATGACAAAGGGCAGGATCCCGACTCCACACCTCTGGAAAACTTTAATATCAAGAACGTCGTAAGA
Peptide sequence:
MLRNEDELEFAKRFELVHIDTKSATQMFELTRRRLTHSEAYPHFMSILHHCLQMPYKRSGNTVQYWLLLDRIIQQIVIQNDKGQDPDSTPLENFNIKNVVRMHLDFFEMLRNEDELEFAKRFELVHIDTKSATQMFELTRRRLTHSEAYPHFMSILHHCLQMPYKRSGNTVQYWLLLDRIIQQIVIQNDKGQDPDSTPLENFNIKNVVR
IRESfinder result
IRESfinder score
0.524700944
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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