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mmu_circDaam1_1013
Basic information of circular RNA
PanCircBase ID:
mmu_circDaam1_1013
mm39_Chr. Position ID:
chr12|71993772|72017952|+
Gene name:
Daam1
Transcript:
ENSMUST00000223272.2
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
8
Exon/intron indexes:
11,12,13,14,15,16,17,18
Exon/intron offsets:
0,394,4195,4834,5453,11748,12807,24095
Genomic length:
24180
Exon/intron sizes in nt:
139,59,188,300,108,27,107,85
Flanking exon/introns:
chr12:71993406-71993772|chr12:72017952-72021963
Splice length:
1013
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
ACAAGAGGAGTGGTAACACAGTTCAGTACTGGCTGCTGTTAGACAGAATCATACAGCAGATAGTTATCCAGAATGACAAAGGGCAGGATCCCGACTCCACACCTCTGGAAAACTTTAATATCAAGAACGTCGTAAGAATGTTGGTTAATGAAAATGAAGTTAAGCAATGGAAAGAGCAGGCAGAGAAGATGAGGAAAGAGCACAATGAGCTACAACAGAAGCTGGAGAAGAAAGAGAGAGAATGTGATGCTAAGACCCAAGAGAAGGAAGAAATGATGCAGACCTTAAACAAGATGAAAGAGAAGCTGGAAAAGGAGACAACTGAGCACAAGCAAGTTAAGCAGCAGGTGGCAGACCTCACGGCGCAGCTCCACGAGCTGAACAGGAGGGCCGTCTGTGCAGCAGTCCCAGGAGGACCCTCACCCGGAGCCCCTGGAGGACCCTTTCCTTCCTCTGGGTTGGGGTCTCTTCTCCCTCCCCCACCACCCCCACTCCTTTCAGGTGGAGCACTTCCTCCTCCACCACCTCCCCTCCCTCCTGGGGGTCCTCCTCCTCCCCCAGGGCCTCCTCCCTTAGGAGGAGTTCTGCCACCTCCTGGTGCTCCAGTGAGTCTAACACTCAAGAAGAAGAATATTCCCCAGCCCACCAATGCCCTGAAATCCTTCAACTGGTCCAAGTTGCCTGAGAACAAATTGGATGGAACAGTATGGACTGAAATTGATGATACCAAAGTCTTCAAAATTCTAGACCTTGAAGACCTAGAAAGAACGTTCTCTGCCTACCAAAGACAGCAGGAGTTCTTTGTGAACAACTCCAAGCAGAAAGAAGCAGATGCCATTGATGACACACTGAGTTCCAAACTTAAAGTCAAAGAGCTGTCAGTGATTGATGGTCGGAGAGCTCAGAATTGCAACATCCTTCTGTCGAGGTTGAAATTATCCAATGATGAAATCAAGCGGGCAATTCTAACAATGGATGAACAGGAGGATCTGCCCAAGGACATGTTAGAACAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GCGGGCAATTCTAACAATGGA
59.25
GGGATCCTGCCCTTTGTCAT
59.74
150
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AGCGGGCAATTCTAACAATGG
59.25
GGGATCCTGCCCTTTGTCAT
59.74
151
PCR template sequence:
CATCCTTCTGTCGAGGTTGAAATTATCCAATGATGAAATCAAGCGGGCAATTCTAACAATGGATGAACAGGAGGATCTGCCCAAGGACATGTTAGAACAGACAAGAGGAGTGGTAACACAGTTCAGTACTGGCTGCTGTTAGACAGAATCATACAGCAGATAGTTATCCAGAATGACAAAGGGCAGGATCCCGACTCCAC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
6
Interacting miRNAs (#binding sites)
mmu-miR-211-5p(1), mmu-miR-204-5p(1), mmu-miR-680(1), mmu-miR-207(1), mmu-miR-335-3p(1), mmu-miR-3105-3p(1)
siRNAs for circular RNA silencing
Junction sequence
CATGTTAGAACAGACAAGAGGAGTGG
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCDAAM1_1013_ORF_1
1.1332
0.226730121
0.987488123
918
ORF sequence:
ATGTTGGTTAATGAAAATGAAGTTAAGCAATGGAAAGAGCAGGCAGAGAAGATGAGGAAAGAGCACAATGAGCTACAACAGAAGCTGGAGAAGAAAGAGAGAGAATGTGATGCTAAGACCCAAGAGAAGGAAGAAATGATGCAGACCTTAAACAAGATGAAAGAGAAGCTGGAAAAGGAGACAACTGAGCACAAGCAAGTTAAGCAGCAGGTGGCAGACCTCACGGCGCAGCTCCACGAGCTGAACAGGAGGGCCGTCTGTGCAGCAGTCCCAGGAGGACCCTCACCCGGAGCCCCTGGAGGACCCTTTCCTTCCTCTGGGTTGGGGTCTCTTCTCCCTCCCCCACCACCCCCACTCCTTTCAGGTGGAGCACTTCCTCCTCCACCACCTCCCCTCCCTCCTGGGGGTCCTCCTCCTCCCCCAGGGCCTCCTCCCTTAGGAGGAGTTCTGCCACCTCCTGGTGCTCCAGTGAGTCTAACACTCAAGAAGAAGAATATTCCCCAGCCCACCAATGCCCTGAAATCCTTCAACTGGTCCAAGTTGCCTGAGAACAAATTGGATGGAACAGTATGGACTGAAATTGATGATACCAAAGTCTTCAAAATTCTAGACCTTGAAGACCTAGAAAGAACGTTCTCTGCCTACCAAAGACAGCAGGAGTTCTTTGTGAACAACTCCAAGCAGAAAGAAGCAGATGCCATTGATGACACACTGAGTTCCAAACTTAAAGTCAAAGAGCTGTCAGTGATTGATGGTCGGAGAGCTCAGAATTGCAACATCCTTCTGTCGAGGTTGAAATTATCCAATGATGAAATCAAGCGGGCAATTCTAACAATGGATGAACAGGAGGATCTGCCCAAGGACATGTTAGAACAGACAAGAGGAGTGGTAACACAGTTCAGTACTGGCTGCTGTTAG
Peptide sequence:
MLVNENEVKQWKEQAEKMRKEHNELQQKLEKKERECDAKTQEKEEMMQTLNKMKEKLEKETTEHKQVKQQVADLTAQLHELNRRAVCAAVPGGPSPGAPGGPFPSSGLGSLLPPPPPPLLSGGALPPPPPPLPPGGPPPPPGPPPLGGVLPPPGAPVSLTLKKKNIPQPTNALKSFNWSKLPENKLDGTVWTEIDDTKVFKILDLEDLERTFSAYQRQQEFFVNNSKQKEADAIDDTLSSKLKVKELSVIDGRRAQNCNILLSRLKLSNDEIKRAILTMDEQEDLPKDMLEQTRGVVTQFSTGCC
IRESfinder result
IRESfinder score
0.519857446
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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