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mmu_circD030028A08Rik_864
Basic information of circular RNA
PanCircBase ID:
mmu_circD030028A08Rik_864
mm39_Chr. Position ID:
chr11|96850379|96851822|+
Gene name:
D030028A08Rik
Transcript:
ENSMUST00000135825.9
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
2
Exon/intron indexes:
4,5
Exon/intron offsets:
0,1307
Genomic length:
1443
Exon/intron sizes in nt:
7,28,136
Flanking exon/introns:
chr11:96843554-96850379|chr11:96851822-96854396
Splice length:
864
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
ATGGAGACTACAGGAGGACTCGGAATCCTCTGGGAGTGGAGTTACAGCTGGTTGAGAGCCACGTGAGTGCTGGGAATCAACCTTGGAACCTCTGGAAAAAGCGGCGAGTCAACCACAACCCATCGCTCAAGCCCCATTGCTGGGCCTGGTGACAACGCATGTCAGTCCTGCCTCAGCCCCCTGAATGCATGTTTACAGATGTGCACCAGAGCACCTGACTCAAGTTTTAAACGATCATTTTGAGCACTCCTTCTCATGGCCATTTCCCTGTAAATCAGCAGAGACACTGGGGAATGGAGCAGTTTGACTCCCATGGCTGCTGGAGTCTCCTTTACTGCCCCGTCTGTCTTGAGCAACACTGGGCTCTGTCTGTTCTTGTCTGCTTTTAGAGTGGGCTGAGCTGGTAACATAAAGGAGCAACAGCTGAGTGGCGGTGATGACGCCTTTAATCCCAGCCCTCAGGAGGCAGAGGTAGGCAGATTTCTGAGTCTCAGGCCAGCCTGGGCTACAGAAAAAAAAAAAGGGAAGGAAGAAAAAAGCAGCAGCAGAGACTGCAAAGTGACCAGAGAGCTAAGCAGCCGGCAGGGCAGGCTACCAAAGCAGCAAGATGGCGGCCACAGGGCAGCTAAAAAGACAAGAGTTGACAGCTGGCAGAATGGCCAGCTGCCCTGACAGCAGCAGAAGCAAAAGCAGCCGTGAGCACACTCCAGAACCCAGAGGTCATCATGGTCAGGACTACTTGGCCTAAGACCTGAAGAGACTCCAGGGGAGAAGCCATTTCCTGCCCACCCCAAATGATGGTGACTCCAGCTTCCCGTAAGATCTGACGATGATCTGCCTCAGTTTCAAAAGCAAGCCAAAAGG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
ATTTCCTGCCCACCCCAAAT
59.88
TGATTCCCAGCACTCACGTG
60.32
167
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
ATTTCCTGCCCACCCCAAAT
59.88
GTGGCTCTCAACCAGCTGTA
59.68
150
PCR template sequence:
AGGGGAGAAGCCATTTCCTGCCCACCCCAAATGATGGTGACTCCAGCTTCCCGTAAGATCTGACGATGATCTGCCTCAGTTTCAAAAGCAAGCCAAAAGGATGGAGACTACAGGAGGACTCGGAATCCTCTGGGAGTGGAGTTACAGCTGGTTGAGAGCCACGTGAGTGCTGGGAATCAACCTTGGAACCTCTGGAAAAA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
13
Interacting miRNAs (#binding sites)
mmu-miR-135a-5p(1), mmu-miR-30e-5p(1), mmu-miR-122-5p(1), mmu-miR-211-5p(1), mmu-miR-30c-5p(1), mmu-miR-18a-5p(1), mmu-miR-30b-5p(1), mmu-miR-136-5p(1), mmu-miR-433-3p(1), mmu-miR-483-5p(1), mmu-miR-207(1), mmu-miR-452-3p(1), mmu-miR-1904(1)
siRNAs for circular RNA silencing
Junction sequence
GCAAGCCAAAAGGATGGAGACTACAG
siRNA sets
TAGTCTCCATCCTTTTGGC, GTAGTCTCCATCCTTTTGG, AGTCTCCATCCTTTTGGCT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
NA
NA
NA
NA
NA
ORF sequence:
NA
Peptide sequence:
NA
IRESfinder result
IRESfinder score
0.417840193
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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