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mmu_circCyfip2_1046
Basic information of circular RNA
PanCircBase ID:
mmu_circCyfip2_1046
mm39_Chr. Position ID:
chr11|46143430|46157656|-
Gene name:
Cyfip2
Transcript:
ENSMUST00000165599.9
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
8
Exon/intron indexes:
19,18,17,16,15,14,13,12
Exon/intron offsets:
0,1343,1906,4945,8196,8833,13300,14106
Genomic length:
14226
Exon/intron sizes in nt:
7,79,71,57,15,41,48,16,00,00,000
Flanking exon/introns:
chr11:46140665-46143430|chr11:46157656-46161378
Splice length:
1046
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTGGTGACAGGCTCAGGGCTGGACAGCCAGAAGTCGGATGAAGAGTACCGGGAGCTTTTTGACCTGGCCCTGCGAGGCCTGCAGCTTCTATCCAAGTGGAGTGCCCATGTCATGGAGGTGTACTCTTGGAAGCTGGTTCACCCCACAGACAAGTTCTGCAACAAGGACTGCCCGGGCACCGCGGAAGAGTACGAGAGAGCCACGCGCTACAATTATACCAGCGAGGAGAAGTTTGCCTTTGTGGAGGTGATTGCCATGATCAAGGGTTTGCAGGTGCTCATGGGAAGGATGGAGAGTGTCTTCAACCAGGCCATCCGCAACACTATCTACGCAGCCCTGCAGGATTTCGCCCAGGTGACGCTGCGGGAGCCGTTGCGGCAAGCAGTTCGAAAGAAAAAGAATGTCCTTATCAGCGTTCTGCAAGCAATTCGAAAGACCATCTGTGACTGGGAGGGAGGCCGAGAGCCCCCCAATGACCCATGCTTAAGAGGGGAGAAGGACCCCAAAGGTGGCTTTGACATCAAGGTGCCCCGCCGTGCTGTGGGTCCATCCAGCACACAGCTGTACATGGTGCGCACCATGCTTGAGTCACTCATCGCAGACAAGAGCGGCTCCAAGAAGACGCTCAGAAGCAGCCTGGATGGCCCTATCGTCCTTGCCATAGAGGACTTCCACAAGCAGTCCTTCTTTTTCACACACCTGCTTAACATCAGCGAGGCTCTTCAGCAGTGCTGTGACCTCTCCCAGCTCTGGTTCCGAGAATTCTTCCTGGAGTTGACCATGGGCCGGCGAATCCAGTTCCCGATTGAGATGTCCATGCCCTGGATTCTAACGGACCATATTCTGGAAACCAAAGAACCTTCCATGATGGAGTATGTCCTCTACCCCTTGGATCTGTACAACGACAGCGCCTACTATGCCCTCACCAAATTCAAAAAGCAGTTCCTGTATGACGAGATCGAAGCCGAGGTAAACCTGTGTTTTGATCAGTTTGTCTACAAGCTGGCTGACCAGATCTTCGCCTACTACAAAGCCATGGCCGGCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GTCTACAAGCTGGCTGACCA
59.68
TTGGATAGAAGCTGCAGGCC
60.11
148
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TGTCTACAAGCTGGCTGACC
59.68
TTGGATAGAAGCTGCAGGCC
60.11
149
PCR template sequence:
TGTATGACGAGATCGAAGCCGAGGTAAACCTGTGTTTTGATCAGTTTGTCTACAAGCTGGCTGACCAGATCTTCGCCTACTACAAAGCCATGGCCGGCAGGTGGTGACAGGCTCAGGGCTGGACAGCCAGAAGTCGGATGAAGAGTACCGGGAGCTTTTTGACCTGGCCCTGCGAGGCCTGCAGCTTCTATCCAAGTGGA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
7
Interacting miRNAs (#binding sites)
mmu-miR-9-5p(1), mmu-miR-135a-5p(1), mmu-miR-216a-5p(1), mmu-miR-125a-5p(1), mmu-miR-29b-2-5p(1), mmu-miR-207(1), mmu-miR-673-5p(1)
siRNAs for circular RNA silencing
Junction sequence
CCATGGCCGGCAGGTGGTGACAGGCT
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCCYFIP2_1046_ORF_1
1.2926
0.490779528
0.997311678
942
ORF sequence:
ATGGAGGTGTACTCTTGGAAGCTGGTTCACCCCACAGACAAGTTCTGCAACAAGGACTGCCCGGGCACCGCGGAAGAGTACGAGAGAGCCACGCGCTACAATTATACCAGCGAGGAGAAGTTTGCCTTTGTGGAGGTGATTGCCATGATCAAGGGTTTGCAGGTGCTCATGGGAAGGATGGAGAGTGTCTTCAACCAGGCCATCCGCAACACTATCTACGCAGCCCTGCAGGATTTCGCCCAGGTGACGCTGCGGGAGCCGTTGCGGCAAGCAGTTCGAAAGAAAAAGAATGTCCTTATCAGCGTTCTGCAAGCAATTCGAAAGACCATCTGTGACTGGGAGGGAGGCCGAGAGCCCCCCAATGACCCATGCTTAAGAGGGGAGAAGGACCCCAAAGGTGGCTTTGACATCAAGGTGCCCCGCCGTGCTGTGGGTCCATCCAGCACACAGCTGTACATGGTGCGCACCATGCTTGAGTCACTCATCGCAGACAAGAGCGGCTCCAAGAAGACGCTCAGAAGCAGCCTGGATGGCCCTATCGTCCTTGCCATAGAGGACTTCCACAAGCAGTCCTTCTTTTTCACACACCTGCTTAACATCAGCGAGGCTCTTCAGCAGTGCTGTGACCTCTCCCAGCTCTGGTTCCGAGAATTCTTCCTGGAGTTGACCATGGGCCGGCGAATCCAGTTCCCGATTGAGATGTCCATGCCCTGGATTCTAACGGACCATATTCTGGAAACCAAAGAACCTTCCATGATGGAGTATGTCCTCTACCCCTTGGATCTGTACAACGACAGCGCCTACTATGCCCTCACCAAATTCAAAAAGCAGTTCCTGTATGACGAGATCGAAGCCGAGGTAAACCTGTGTTTTGATCAGTTTGTCTACAAGCTGGCTGACCAGATCTTCGCCTACTACAAAGCCATGGCCGGCAGGTGGTGA
Peptide sequence:
MEVYSWKLVHPTDKFCNKDCPGTAEEYERATRYNYTSEEKFAFVEVIAMIKGLQVLMGRMESVFNQAIRNTIYAALQDFAQVTLREPLRQAVRKKKNVLISVLQAIRKTICDWEGGREPPNDPCLRGEKDPKGGFDIKVPRRAVGPSSTQLYMVRTMLESLIADKSGSKKTLRSSLDGPIVLAIEDFHKQSFFFTHLLNISEALQQCCDLSQLWFREFFLELTMGRRIQFPIEMSMPWILTDHILETKEPSMMEYVLYPLDLYNDSAYYALTKFKKQFLYDEIEAEVNLCFDQFVYKLADQIFAYYKAMAGRW
IRESfinder result
IRESfinder score
0.337752906
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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