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mmu_circCyb5r3_712
Basic information of circular RNA
PanCircBase ID:
mmu_circCyb5r3_712
mm39_Chr. Position ID:
chr15|83043026|83050407|-
Gene name:
Cyb5r3
Transcript:
ENSMUST00000162834.3
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
7
Exon/intron indexes:
8,7,6,5,4,3,2
Exon/intron offsets:
0,1278,1512,1995,3024,3406,7249
Genomic length:
7381
Exon/intron sizes in nt:
100,86,84,130,107,73,132
Flanking exon/introns:
chr15:83038923-83043026|chr15:83050407-83054337
Splice length:
712
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
TTGAGCCACGTGGTGCTCTCGCCCGTCTGGTTCATCTACAGTCTCTTCATGAAGCTGTTTCAGCGCTCCACACCGGCCATCACCCTCGAGAACCCCGACATCAAGTACCCTCTGAGGCTCATCGACAAGGAGGTCATCAGTCCTGACACTCGGCGCTTCCGATTTGCCCTCCCTTCACCCCAGCACATCCTGGGCCTTCCTATTGGCCAACACATCTACCTCTCGACTAGGATCGATGGCAACTTGGTCATTCGGCCCTACACCCCTGTGTCGAGCGATGATGACAAGGGCTTTGTGGACTTGGTGGTCAAGGTTTACTTCAAGGACACCCATCCCAAGTTTCCAGCTGGAGGGAAAATGTCTCAGTACCTGGAAAACATGAAGATTGGAGACACCATTGAATTCCGGGGTCCCAATGGGCTACTGGTCTACCAGGGCAAAGGGAAGTTTGCCATCCGTGCAGACAAGAAGTCCAACCCCGTTGTCAGGACGGTGAAGTCTGTAGGCATGATTGCAGGAGGGACAGGCATCACCCCAATGCTGCAGGTGATCCGAGCCGTCCTGAAGGACCCAAACGACCACACCGTGTGCTATCTGCTCTTTGCCAACCAGTCCGAGAAAGATATCCTGTTGCGGCCTGAGCTGGAGGAACTGAGGAACGAACATTCTGCTCGCTTCAAGCTCTGGTACACAGTGGACAAAGCCCCTGATG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GGAACGAACATTCTGCTCGC
59.9
GTTCTCGAGGGTGATGGCC
60.15
150
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GGAACGAACATTCTGCTCGC
59.9
GGTTCTCGAGGGTGATGGC
60.15
151
PCR template sequence:
TCCGAGAAAGATATCCTGTTGCGGCCTGAGCTGGAGGAACTGAGGAACGAACATTCTGCTCGCTTCAAGCTCTGGTACACAGTGGACAAAGCCCCTGATGTTGAGCCACGTGGTGCTCTCGCCCGTCTGGTTCATCTACAGTCTCTTCATGAAGCTGTTTCAGCGCTCCACACCGGCCATCACCCTCGAGAACCCCGACA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
12
Interacting miRNAs (#binding sites)
mmu-let-7a-5p(1), mmu-miR-103-3p(1), mmu-let-7e-5p(1), mmu-let-7b-5p(1), mmu-let-7c-5p(1), mmu-miR-211-5p(1), mmu-miR-204-5p(1), mmu-let-7d-5p(1), mmu-miR-483-5p(1), mmu-miR-182-5p(1), mmu-miR-107-3p(1), mmu-miR-98-5p(1)
siRNAs for circular RNA silencing
Junction sequence
AAAGCCCCTGATGTTGAGCCACGTGG
siRNA sets
ACGTGGCTCAACATCAGGG, CACGTGGCTCAACATCAGG, CGTGGCTCAACATCAGGGG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCCYB5R3_712_ORF_1
1.2591
0.44927106
0.991398579
810
ORF sequence:
ATGAAGCTGTTTCAGCGCTCCACACCGGCCATCACCCTCGAGAACCCCGACATCAAGTACCCTCTGAGGCTCATCGACAAGGAGGTCATCAGTCCTGACACTCGGCGCTTCCGATTTGCCCTCCCTTCACCCCAGCACATCCTGGGCCTTCCTATTGGCCAACACATCTACCTCTCGACTAGGATCGATGGCAACTTGGTCATTCGGCCCTACACCCCTGTGTCGAGCGATGATGACAAGGGCTTTGTGGACTTGGTGGTCAAGGTTTACTTCAAGGACACCCATCCCAAGTTTCCAGCTGGAGGGAAAATGTCTCAGTACCTGGAAAACATGAAGATTGGAGACACCATTGAATTCCGGGGTCCCAATGGGCTACTGGTCTACCAGGGCAAAGGGAAGTTTGCCATCCGTGCAGACAAGAAGTCCAACCCCGTTGTCAGGACGGTGAAGTCTGTAGGCATGATTGCAGGAGGGACAGGCATCACCCCAATGCTGCAGGTGATCCGAGCCGTCCTGAAGGACCCAAACGACCACACCGTGTGCTATCTGCTCTTTGCCAACCAGTCCGAGAAAGATATCCTGTTGCGGCCTGAGCTGGAGGAACTGAGGAACGAACATTCTGCTCGCTTCAAGCTCTGGTACACAGTGGACAAAGCCCCTGATGTTGAGCCACGTGGTGCTCTCGCCCGTCTGGTTCATCTACAGTCTCTTCATGAAGCTGTTTCAGCGCTCCACACCGGCCATCACCCTCGAGAACCCCGACATCAAGTACCCTCTGAGGCTCATCGACAAGGAGGTCATCAGTCCTGA
Peptide sequence:
MKLFQRSTPAITLENPDIKYPLRLIDKEVISPDTRRFRFALPSPQHILGLPIGQHIYLSTRIDGNLVIRPYTPVSSDDDKGFVDLVVKVYFKDTHPKFPAGGKMSQYLENMKIGDTIEFRGPNGLLVYQGKGKFAIRADKKSNPVVRTVKSVGMIAGGTGITPMLQVIRAVLKDPNDHTVCYLLFANQSEKDILLRPELEELRNEHSARFKLWYTVDKAPDVEPRGALARLVHLQSLHEAVSALHTGHHPREPRHQVPSEAHRQGGHQS
IRESfinder result
IRESfinder score
0.449239216
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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