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mmu_circCyb5b_159
Basic information of circular RNA
PanCircBase ID:
mmu_circCyb5b_159
mm39_Chr. Position ID:
chr8|107896442|107897052|+
Gene name:
Cyb5b
Transcript:
ENSMUST00000034400.5
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
2
Exon/intron indexes:
2,3
Exon/intron offsets:
0,580
Genomic length:
610
Exon/intron sizes in nt:
129,30
Flanking exon/introns:
chr8:107877505-107896442|chr8:107897052-107906045
Splice length:
159
Other database IDs:
CIRCpedia: MMU_CIRCpedia_2037, circAtlas: mmu-Cyb5b_0001
circAtlas: hsa-SCAF8_0001, CIRCpedia: HSA_CIRCpedia_18388
Human Ortholog IDs:
Mature spliced Sequence:
CATCCTGGCGGGGAAGAGGTTCTGCTGGAACAAGCGGGTGCTGATGCAACTGAAAGCTTTGAAGATGTCGGCCACTCCCCTGATGCCAGGGAGATGCTAAAGCAGTACTACATTGGAGATGTCCATCCGAGTGACCTTAAACCTAAAGGCGATGACAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TGCCAGGGAGATGCTAAAGC
60.11
GGAGTGGCCGACATCTTCAA
60.04
154
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TGCCAGGGAGATGCTAAAGC
60.11
TGGCCGACATCTTCAAAGCT
59.96
150
PCR template sequence:
TGATGCCAGGGAGATGCTAAAGCAGTACTACATTGGAGATGTCCATCCGAGTGACCTTAAACCTAAAGGCGATGACAAGCATCCTGGCGGGGAAGAGGTTCTGCTGGAACAAGCGGGTGCTGATGCAACTGAAAGCTTTGAAGATGTCGGCCACTCCC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
NA
Interacting miRNAs (#binding sites)
NA
siRNAs for circular RNA silencing
Junction sequence
AGGCGATGACAAGCATCCTGGCGGGG
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCCYB5B_159_ORF_1
1.2225
0.406470694
0.576499824
225
ORF sequence:
ATGCTAAAGCAGTACTACATTGGAGATGTCCATCCGAGTGACCTTAAACCTAAAGGCGATGACAAGCATCCTGGCGGGGAAGAGGTTCTGCTGGAACAAGCGGGTGCTGATGCAACTGAAAGCTTTGAAGATGTCGGCCACTCCCCTGATGCCAGGGAGATGCTAAAGCAGTACTACATTGGAGATGTCCATCCGAGTGACCTTAAACCTAAAGGCGATGACAAG
Peptide sequence:
MLKQYYIGDVHPSDLKPKGDDKHPGGEEVLLEQAGADATESFEDVGHSPDAREMLKQYYIGDVHPSDLKPKGDDK
IRESfinder result
IRESfinder score
0.053804308
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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