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mmu_circCwf19l2_919
Basic information of circular RNA
PanCircBase ID:
mmu_circCwf19l2_919
mm39_Chr. Position ID:
chr9|3441054|3458889|+
Gene name:
Cwf19l2
Transcript:
ENSMUST00000027027.7
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
7
Exon/intron indexes:
9,10,11,12,13,14,15
Exon/intron offsets:
0,1906,6182,8959,13485,15678,17679
Genomic length:
17835
Exon/intron sizes in nt:
91,87,11,71,38,21,31,10,000
Flanking exon/introns:
chr9:3431087-3441054|chr9:3458889-3460050
Splice length:
919
Other database IDs:
CIRCpedia: MMU_CIRCpedia_216880, circAtlas: mmu-Cwf19l2_0020
Human Ortholog IDs:
Mature spliced Sequence:
TCCAGAGGCAGAGTCCACCTATATCTTAAATGTTGATGAAAAGAACAAGTTGGGGGCCAAGATTATCAAGGCAGAAATGATGGGGAATATGGAATTAGCTGAACAACTTAAAGCCCAACTTAAAGAGGCAAATAAATTTAAAGAAACACAGATGCCGGCAAAAAGATTGGGCGTAGAGCATGAAGATGAACAAGAAGTAATCCTTATTCAAACAGATAAGTCTGGAAGAATGTGGCCTGTGAGCAGCCCTAGAGAAACCCTGGATATGAAAGCAGAACGAAGGAAGAGAAAGAGAGTTTCAACCCATGAGGATAAAGAAAGGGTCCGATACTTTCCTGATGATGATCATTTGAGCCTCAAAGATTTAGTCAAGAATGAGAAAATAGGAACAGATATCAACCAAAACAGACTTTTCATGAAAATGGCATCTAAGTTTATGGGAAAATCAGATGAAGACAATTATACTCTGGATGACATGTTTGTCTCCAAAGCAGCTGAGAAAGAGCATCTTGGTAAGAAGGAAGAGAGCCAGAGGAGAAGAGCGATTGCTGAGCACCAGAGTCTTGCTGCAAAAATGGCAAAGTGCCTTTACTGTTTTGACAGCTCTCAGTTCCCCAAGCACCTTATTGTTGCAATCGGTGTGAAGGTTTATTTATGTTTACCCAGTTTCCAGTCTCTTACTGAAGGACACTGCTTCATAGTCCCTCTGCAACATCATCAAGCAGCCACTGTATTAGATGAAGATGTCTGGGAGGAGATCCAGATGTTTCGAAAATCATTGGTAAAAATGTTTGAAGATAAAGAATTGGATTGTATCTTTTTGGAGACTAATATGGGTTTGAAGAAACACTATCATATGGTTTATGAGTGTATCCCTCTCCCAAAGGAAGTGGGTGATATGGCTCCCATCTATTTTAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GAGTGTATCCCTCTCCCAAAGG
59.56
TCCCCATCATTTCTGCCTTGA
59.36
140
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AGTGTATCCCTCTCCCAAAGGA
59.95
TTCCCCATCATTTCTGCCTTGA
59.96
140
PCR template sequence:
TTTGGAGACTAATATGGGTTTGAAGAAACACTATCATATGGTTTATGAGTGTATCCCTCTCCCAAAGGAAGTGGGTGATATGGCTCCCATCTATTTTAAGTCCAGAGGCAGAGTCCACCTATATCTTAAATGTTGATGAAAAGAACAAGTTGGGGGCCAAGATTATCAAGGCAGAAATGATGGGGAATATGGAATTAGCT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
6
Interacting miRNAs (#binding sites)
mmu-miR-129-5p(2), mmu-miR-30c-5p(1), mmu-miR-149-5p(1), mmu-miR-382-5p(1), mmu-miR-207(1), mmu-miR-1904(1)
siRNAs for circular RNA silencing
Junction sequence
CATCTATTTTAAGTCCAGAGGCAGAG
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCCWF19L2_919_ORF_1
0.9259
0.087169022
0.978711917
975
ORF sequence:
ATGAGTGTATCCCTCTCCCAAAGGAAGTGGGTGATATGGCTCCCATCTATTTTAAGTCCAGAGGCAGAGTCCACCTATATCTTAAATGTTGATGAAAAGAACAAGTTGGGGGCCAAGATTATCAAGGCAGAAATGATGGGGAATATGGAATTAGCTGAACAACTTAAAGCCCAACTTAAAGAGGCAAATAAATTTAAAGAAACACAGATGCCGGCAAAAAGATTGGGCGTAGAGCATGAAGATGAACAAGAAGTAATCCTTATTCAAACAGATAAGTCTGGAAGAATGTGGCCTGTGAGCAGCCCTAGAGAAACCCTGGATATGAAAGCAGAACGAAGGAAGAGAAAGAGAGTTTCAACCCATGAGGATAAAGAAAGGGTCCGATACTTTCCTGATGATGATCATTTGAGCCTCAAAGATTTAGTCAAGAATGAGAAAATAGGAACAGATATCAACCAAAACAGACTTTTCATGAAAATGGCATCTAAGTTTATGGGAAAATCAGATGAAGACAATTATACTCTGGATGACATGTTTGTCTCCAAAGCAGCTGAGAAAGAGCATCTTGGTAAGAAGGAAGAGAGCCAGAGGAGAAGAGCGATTGCTGAGCACCAGAGTCTTGCTGCAAAAATGGCAAAGTGCCTTTACTGTTTTGACAGCTCTCAGTTCCCCAAGCACCTTATTGTTGCAATCGGTGTGAAGGTTTATTTATGTTTACCCAGTTTCCAGTCTCTTACTGAAGGACACTGCTTCATAGTCCCTCTGCAACATCATCAAGCAGCCACTGTATTAGATGAAGATGTCTGGGAGGAGATCCAGATGTTTCGAAAATCATTGGTAAAAATGTTTGAAGATAAAGAATTGGATTGTATCTTTTTGGAGACTAATATGGGTTTGAAGAAACACTATCATATGGTTTATGAGTGTATCCCTCTCCCAAAGGAAGTGGGTGATATGGCTCCCATCTATTTTAAG
Peptide sequence:
MSVSLSQRKWVIWLPSILSPEAESTYILNVDEKNKLGAKIIKAEMMGNMELAEQLKAQLKEANKFKETQMPAKRLGVEHEDEQEVILIQTDKSGRMWPVSSPRETLDMKAERRKRKRVSTHEDKERVRYFPDDDHLSLKDLVKNEKIGTDINQNRLFMKMASKFMGKSDEDNYTLDDMFVSKAAEKEHLGKKEESQRRRAIAEHQSLAAKMAKCLYCFDSSQFPKHLIVAIGVKVYLCLPSFQSLTEGHCFIVPLQHHQAATVLDEDVWEEIQMFRKSLVKMFEDKELDCIFLETNMGLKKHYHMVYECIPLPKEVGDMAPIYFK
IRESfinder result
IRESfinder score
0.584388337
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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