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mmu_circCwf19l1_854
Basic information of circular RNA
PanCircBase ID:
mmu_circCwf19l1_854
mm39_Chr. Position ID:
chr19|44109311|44119952|-
Gene name:
Cwf19l1
Transcript:
ENSMUST00000026218.7
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
7
Exon/intron indexes:
10,9,8,7,6,5,4
Exon/intron offsets:
0,976,2271,3935,6493,8325,10539
Genomic length:
10641
Exon/intron sizes in nt:
80,11,21,41,85,11,92,10,000
Flanking exon/introns:
chr19:44103172-44109311|chr19:44119952-44120524
Splice length:
854
Other database IDs:
circAtlas: hsa-SCAF8_0001
Human Ortholog IDs:
Mature spliced Sequence:
CTCCTATTCAGACATATGTGCTTGGTGCCAATAACGAGGAGACAGCAAACTATTTCCAGGGTGCTGATGGCTGTGAATTGGCTGAAAACATCACTTACCTGGGTCGGAAAGGTGTCTTCACTGGAAGCTCGGGGCTACAGATTGTATACCTCAGTGGGACCGAGTCCTTAGATGAACCAGTCCCAGCCCACAGCTTTAGTCCCAAGGATGTGTCTTCTCTGAGAACAATGCTGTGCTCGGCTTCCCAGTTTAAAGGTGTTGATATCTTACTCACGTCCCCGTGGCCCAAGTACGTGGGGAGCTTTGGGAATTCCTCTGGAGAAGTAGACACCAAAAACTGTGGCTCAGCTTTGATCTCCAGCCTCGCCGTGAGCTTGAAACCGAGGTATCATTTTGCTGCTTTGGAAAAGTCGTATTATGAGAGGCTTCCGTACCGAAACCATGTTGTTCTACAAGAAAGCGCGCAGCATGCCACTCGCTTCATAGCGCTGGCGAATGTTGGAAATCCAGAAAAGAAGAAGTATCTTTATGCCTTCAGTATTGTTCCCATGAAGCTGATGGCTGTAGCCGAACTGGTAAAGCAGCCTCCGGACGTCACCGAGAACCCTTACCGAGACTCTGGGAAGCAGGCAGCCGGAGGGAAACACATTCCCGCCCCTCAGGAAGAGTCAGCCTGTCAGTTCTTCTTCGATTTGAGTGAAAAGCAGGGAAGGAAGCGGCCGTCCACAGGCAGGGATACCAGGCCTCCCCACGCTAAGCAGCCTCGCAAGCCTCCTCAGCCTCCAGGCCCTTGTTGGTTTTGCCTCGCCAGCCCTGAAGTGGAAAAGCACCTGGTGGTCAACATTGGCACACAT
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TCCAGGCCCTTGTTGGTTTT
60.03
GCCAATTCACAGCCATCAGC
60.18
155
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CCCTTGTTGGTTTTGCCTCG
59.97
GCCAATTCACAGCCATCAGC
60.18
149
PCR template sequence:
TAAGCAGCCTCGCAAGCCTCCTCAGCCTCCAGGCCCTTGTTGGTTTTGCCTCGCCAGCCCTGAAGTGGAAAAGCACCTGGTGGTCAACATTGGCACACATCTCCTATTCAGACATATGTGCTTGGTGCCAATAACGAGGAGACAGCAAACTATTTCCAGGGTGCTGATGGCTGTGAATTGGCTGAAAACATCACTTACCT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
11
Interacting miRNAs (#binding sites)
mmu-miR-320-3p(2), mmu-miR-15a-5p(1), mmu-miR-200b-3p(1), mmu-miR-200c-3p(1), mmu-miR-322-5p(1), mmu-miR-15b-5p(1), mmu-miR-96-5p(1), mmu-miR-362-5p(1), mmu-miR-29b-2-5p(1), mmu-miR-1198-5p(1), mmu-miR-673-5p(1)
siRNAs for circular RNA silencing
Junction sequence
CATTGGCACACATCTCCTATTCAGAC
siRNA sets
TGAATAGGAGATGTGTGCC, CTGAATAGGAGATGTGTGC, GAATAGGAGATGTGTGCCA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCCWF19L1_854_ORF_1
0.7823
0.150787704
0.927363946
798
ORF sequence:
ATGCTGTGCTCGGCTTCCCAGTTTAAAGGTGTTGATATCTTACTCACGTCCCCGTGGCCCAAGTACGTGGGGAGCTTTGGGAATTCCTCTGGAGAAGTAGACACCAAAAACTGTGGCTCAGCTTTGATCTCCAGCCTCGCCGTGAGCTTGAAACCGAGGTATCATTTTGCTGCTTTGGAAAAGTCGTATTATGAGAGGCTTCCGTACCGAAACCATGTTGTTCTACAAGAAAGCGCGCAGCATGCCACTCGCTTCATAGCGCTGGCGAATGTTGGAAATCCAGAAAAGAAGAAGTATCTTTATGCCTTCAGTATTGTTCCCATGAAGCTGATGGCTGTAGCCGAACTGGTAAAGCAGCCTCCGGACGTCACCGAGAACCCTTACCGAGACTCTGGGAAGCAGGCAGCCGGAGGGAAACACATTCCCGCCCCTCAGGAAGAGTCAGCCTGTCAGTTCTTCTTCGATTTGAGTGAAAAGCAGGGAAGGAAGCGGCCGTCCACAGGCAGGGATACCAGGCCTCCCCACGCTAAGCAGCCTCGCAAGCCTCCTCAGCCTCCAGGCCCTTGTTGGTTTTGCCTCGCCAGCCCTGAAGTGGAAAAGCACCTGGTGGTCAACATTGGCACACATCTCCTATTCAGACATATGTGCTTGGTGCCAATAACGAGGAGACAGCAAACTATTTCCAGGGTGCTGATGGCTGTGAATTGGCTGAAAACATCACTTACCTGGGTCGGAAAGGTGTCTTCACTGGAAGCTCGGGGCTACAGATTGTATACCTCAGTGGGACCGAGTCCTTAG
Peptide sequence:
MLCSASQFKGVDILLTSPWPKYVGSFGNSSGEVDTKNCGSALISSLAVSLKPRYHFAALEKSYYERLPYRNHVVLQESAQHATRFIALANVGNPEKKKYLYAFSIVPMKLMAVAELVKQPPDVTENPYRDSGKQAAGGKHIPAPQEESACQFFFDLSEKQGRKRPSTGRDTRPPHAKQPRKPPQPPGPCWFCLASPEVEKHLVVNIGTHLLFRHMCLVPITRRQQTISRVLMAVNWLKTSLTWVGKVSSLEARGYRLYTSVGPSP
IRESfinder result
IRESfinder score
0.491898999
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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