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mmu_circCryzl1_803
Basic information of circular RNA
PanCircBase ID:
mmu_circCryzl1_803
mm39_Chr. Position ID:
chr16|91487591|91509155|-
Gene name:
Cryzl1
Transcript:
ENSMUST00000073466.13
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
9
Exon/intron indexes:
12,11,10,9,8,7,6,5,4
Exon/intron offsets:
0,1895,3478,4557,8473,10368,16521,18125,21491
Genomic length:
21564
Exon/intron sizes in nt:
46,106,119,99,112,134,69,45,73
Flanking exon/introns:
chr16:91486803-91487591|chr16:91509155-91511636
Splice length:
803
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
CTTCTAGCAGAAATGAAGATGGAAAAGGATTTCTTCCCTGTTGGAAGAGAAGTTTCTGGAATTGTATTAGAGGTTGGCAGGAAGGTAACGTTCTTTCAGCCAGACGACGAAGTGGTCGGAATTCTGCCTTTGGATTCCGAAGACCCCGGGCTCTGTGAGGTTATTCGAGTACATGAGCATTACCTGGTTCATAAGCCAGAAAAGGTGTCATGGACGGAAGCTGCTGGCGTCATCCGGGACGGAGTCCGAGCCTGCACAGCTCTGTATTATCTTTCTCAACTCTCTCCTGGAAAGTCTGTGCTGATCATGGACGGGGCAAGTGCATTTGGCACAATAGCCATTCAGTTAGCCCACCACAGAGGAGCCAAGGTTATCTCAACAGCACACAGCCTGGAGGACAAGCAACATCTTGAAAGGCTTAGGCCTTCTATAGCCCGTGTGATTGATGTGTCTAATGGGAAAGTCCATGTTGCTGAAAGCTGTTTGGAGGAAACAGGTGGCCTGGGAGTAGATATTGTCATAGATGCAGGAGTGAGATTATATAGTAAAGATGATGAACCAGCTGTAAAGTTACATCTGCCACATAAGCATGACATCATCACACTTCTTGGTGTCGGAGGCCATTGGGTAACAACAGAAGAAAACCTTCAGTTGGATCCTCCAGATAGCCACTGCCTTTTCCTCAAGGGAGCAACGGTGGCTTTCCTTAATGATGAAGTTTGGAATTTGTCAAATGCACAACAGGGAAAATATCTTTGTATCTTAAAAGATGTGATGGAGAAGTTATCAGCTGGTGTTTTCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TGTCAAATGCACAACAGGGA
57.94
CGTTACCTTCCTGCCAACCT
59.96
166
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TGTCAAATGCACAACAGGGA
57.94
ACGTTACCTTCCTGCCAACC
60.25
167
PCR template sequence:
TCCTTAATGATGAAGTTTGGAATTTGTCAAATGCACAACAGGGAAAATATCTTTGTATCTTAAAAGATGTGATGGAGAAGTTATCAGCTGGTGTTTTCAGCTTCTAGCAGAAATGAAGATGGAAAAGGATTTCTTCCCTGTTGGAAGAGAAGTTTCTGGAATTGTATTAGAGGTTGGCAGGAAGGTAACGTTCTTTCAGC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
9
Interacting miRNAs (#binding sites)
mmu-miR-155-5p(1), mmu-miR-34c-5p(1), mmu-miR-34a-5p(1), mmu-miR-92a-3p(1), mmu-miR-216a-5p(1), mmu-miR-196a-5p(1), mmu-miR-34b-5p(1), mmu-miR-369-3p(1), mmu-miR-484(1)
siRNAs for circular RNA silencing
Junction sequence
CTGGTGTTTTCAGCTTCTAGCAGAAA
siRNA sets
CTAGAAGCTGAAAACACCA, GCTAGAAGCTGAAAACACC, TAGAAGCTGAAAACACCAG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCCRYZL1_803_ORF_1
1.0438
0.109812132
0.94624471
798
ORF sequence:
ATGAAGATGGAAAAGGATTTCTTCCCTGTTGGAAGAGAAGTTTCTGGAATTGTATTAGAGGTTGGCAGGAAGGTAACGTTCTTTCAGCCAGACGACGAAGTGGTCGGAATTCTGCCTTTGGATTCCGAAGACCCCGGGCTCTGTGAGGTTATTCGAGTACATGAGCATTACCTGGTTCATAAGCCAGAAAAGGTGTCATGGACGGAAGCTGCTGGCGTCATCCGGGACGGAGTCCGAGCCTGCACAGCTCTGTATTATCTTTCTCAACTCTCTCCTGGAAAGTCTGTGCTGATCATGGACGGGGCAAGTGCATTTGGCACAATAGCCATTCAGTTAGCCCACCACAGAGGAGCCAAGGTTATCTCAACAGCACACAGCCTGGAGGACAAGCAACATCTTGAAAGGCTTAGGCCTTCTATAGCCCGTGTGATTGATGTGTCTAATGGGAAAGTCCATGTTGCTGAAAGCTGTTTGGAGGAAACAGGTGGCCTGGGAGTAGATATTGTCATAGATGCAGGAGTGAGATTATATAGTAAAGATGATGAACCAGCTGTAAAGTTACATCTGCCACATAAGCATGACATCATCACACTTCTTGGTGTCGGAGGCCATTGGGTAACAACAGAAGAAAACCTTCAGTTGGATCCTCCAGATAGCCACTGCCTTTTCCTCAAGGGAGCAACGGTGGCTTTCCTTAATGATGAAGTTTGGAATTTGTCAAATGCACAACAGGGAAAATATCTTTGTATCTTAAAAGATGTGATGGAGAAGTTATCAGCTGGTGTTTTCAGCTTCTAG
Peptide sequence:
MKMEKDFFPVGREVSGIVLEVGRKVTFFQPDDEVVGILPLDSEDPGLCEVIRVHEHYLVHKPEKVSWTEAAGVIRDGVRACTALYYLSQLSPGKSVLIMDGASAFGTIAIQLAHHRGAKVISTAHSLEDKQHLERLRPSIARVIDVSNGKVHVAESCLEETGGLGVDIVIDAGVRLYSKDDEPAVKLHLPHKHDIITLLGVGGHWVTTEENLQLDPPDSHCLFLKGATVAFLNDEVWNLSNAQQGKYLCILKDVMEKLSAGVFSF
IRESfinder result
IRESfinder score
0.574704013
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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