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mmu_circCrybg3_1003
Basic information of circular RNA
PanCircBase ID:
mmu_circCrybg3_1003
mm39_Chr. Position ID:
chr16|59349585|59370912|-
Gene name:
Crybg3
Transcript:
ENSMUST00000044604.16
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
9
Exon/intron indexes:
12,11,10,9,8,7,6,5,4
Exon/intron offsets:
0,881,1108,7475,10582,12837,13953,14806,21179
Genomic length:
21327
Exon/intron sizes in nt:
33,12,71,46,12,71,34,30,00,00,00,000
Flanking exon/introns:
chr16:59345333-59349585|chr16:59370912-59372534
Splice length:
1003
Other database IDs:
circAtlas: mmu-Crybg3_0018
Human Ortholog IDs:
Mature spliced Sequence:
CTGGATTCTGTATGAGAAGCCCCATTTCCAAGGTCAGAAGTGTGTGTTGGAAGAAGGGGAAAGAGTGTTAGACCGGGACTGGCTTCTTCAGAACAGGAAGCACCCAGAAAGGAACTTTGTCCTGGGCTCTATCAAACGTGTCTTAAAGGATTGCAGCATTCCGGTGATAGAGCTGTGCCCCAAGACTGACCCTGGGTGCAGCCCCATCTACATACACCGATCTGTCCCCAATGTGGAAGAGCTGAACATCCCCAAGTCTACATCCGTCACTGTGAAGTCAGGCGTTTGGCTGGCCTACCCAGATATTCATTTTAAGGGGCAAGCTACAATTTTGGAGGAAGACCAGGGGCTCTTTGAGATTTCTGCAGCCGAGATGAAATCACTGCATCCTCTTCAAATGGGTGGACTGAAAGTGGAAATGCCTATGAACTTAAAGGTTATCCTTTATGAGAAACCTCACTTCCTCGGACATACCAAAGAGTTTAGTGAACATATAGATTCTGTCCCTACGTTTTTAAAAAGCGATAAGGACTTCCATGGCATCGGATCGATTCGTGTCATTGGTGGAGTGTGGGTTGCCTATGAAAAGGAACATTTCAAAGGCCAGCAATTCCTACTAGAAGAAGGAGACTTTGAAGATAGCAGCGCTTGTGGGGCCTTAAGTGGACCCATCATGTCTTTCCGGTACCTACAAGCTAATTTTATAGAATCTTCGATCACACTGTTTGAATCATCTCACCTGGAAAGTGGGAAGTTTATTGACATCACGAATCAGGAAATTTCTGACTTAGAAGAAATCGGCTTTGGCAGCGAGACAAGATCCATTCACGTGAAGAGTGGAGTGTGGGTTGCCTACCACCAGAAGTTCTTCTGCGGCGATCAGTACATTTTAGAGAAAGGAAAATATAAATGCTTTTTTGATTGGGGAGGATCAAGTAATACAATCTTGTCTATCCGACCAATCCAATTGGAACCACTCGGGATAAATGAGCCTACACACTTG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TCCAATTGGAACCACTCGGG
59.96
TTCCTGTTCTGAAGAAGCCAGT
59.56
140
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CCAATTGGAACCACTCGGGA
59.96
CTTCCTGTTCTGAAGAAGCCAG
58.93
140
PCR template sequence:
ATATAAATGCTTTTTTGATTGGGGAGGATCAAGTAATACAATCTTGTCTATCCGACCAATCCAATTGGAACCACTCGGGATAAATGAGCCTACACACTTGCTGGATTCTGTATGAGAAGCCCCATTTCCAAGGTCAGAAGTGTGTGTTGGAAGAAGGGGAAAGAGTGTTAGACCGGGACTGGCTTCTTCAGAACAGGAAG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
6
Interacting miRNAs (#binding sites)
mmu-miR-27b-3p(1), mmu-miR-27a-3p(1), mmu-miR-216a-5p(1), mmu-miR-203-3p(1), mmu-miR-128-3p(1), mmu-miR-425-5p(1)
siRNAs for circular RNA silencing
Junction sequence
GCCTACACACTTGCTGGATTCTGTAT
siRNA sets
ATACAGAATCCAGCAAGTG, TACAGAATCCAGCAAGTGT, GAATCCAGCAAGTGTGTAG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCCRYBG3_1003_ORF_1
0.9037
0.141064603
0.986977467
1020
ORF sequence:
ATGAGCCTACACACTTGCTGGATTCTGTATGAGAAGCCCCATTTCCAAGGTCAGAAGTGTGTGTTGGAAGAAGGGGAAAGAGTGTTAGACCGGGACTGGCTTCTTCAGAACAGGAAGCACCCAGAAAGGAACTTTGTCCTGGGCTCTATCAAACGTGTCTTAAAGGATTGCAGCATTCCGGTGATAGAGCTGTGCCCCAAGACTGACCCTGGGTGCAGCCCCATCTACATACACCGATCTGTCCCCAATGTGGAAGAGCTGAACATCCCCAAGTCTACATCCGTCACTGTGAAGTCAGGCGTTTGGCTGGCCTACCCAGATATTCATTTTAAGGGGCAAGCTACAATTTTGGAGGAAGACCAGGGGCTCTTTGAGATTTCTGCAGCCGAGATGAAATCACTGCATCCTCTTCAAATGGGTGGACTGAAAGTGGAAATGCCTATGAACTTAAAGGTTATCCTTTATGAGAAACCTCACTTCCTCGGACATACCAAAGAGTTTAGTGAACATATAGATTCTGTCCCTACGTTTTTAAAAAGCGATAAGGACTTCCATGGCATCGGATCGATTCGTGTCATTGGTGGAGTGTGGGTTGCCTATGAAAAGGAACATTTCAAAGGCCAGCAATTCCTACTAGAAGAAGGAGACTTTGAAGATAGCAGCGCTTGTGGGGCCTTAAGTGGACCCATCATGTCTTTCCGGTACCTACAAGCTAATTTTATAGAATCTTCGATCACACTGTTTGAATCATCTCACCTGGAAAGTGGGAAGTTTATTGACATCACGAATCAGGAAATTTCTGACTTAGAAGAAATCGGCTTTGGCAGCGAGACAAGATCCATTCACGTGAAGAGTGGAGTGTGGGTTGCCTACCACCAGAAGTTCTTCTGCGGCGATCAGTACATTTTAGAGAAAGGAAAATATAAATGCTTTTTTGATTGGGGAGGATCAAGTAATACAATCTTGTCTATCCGACCAATCCAATTGGAACCACTCGGGATAAATGAGCCTACACACTTG
Peptide sequence:
MSLHTCWILYEKPHFQGQKCVLEEGERVLDRDWLLQNRKHPERNFVLGSIKRVLKDCSIPVIELCPKTDPGCSPIYIHRSVPNVEELNIPKSTSVTVKSGVWLAYPDIHFKGQATILEEDQGLFEISAAEMKSLHPLQMGGLKVEMPMNLKVILYEKPHFLGHTKEFSEHIDSVPTFLKSDKDFHGIGSIRVIGGVWVAYEKEHFKGQQFLLEEGDFEDSSACGALSGPIMSFRYLQANFIESSITLFESSHLESGKFIDITNQEISDLEEIGFGSETRSIHVKSGVWVAYHQKFFCGDQYILEKGKYKCFFDWGGSSNTILSIRPIQLEPLGINEPTHL
IRESfinder result
IRESfinder score
0.668699996
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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