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mmu_circCrppa_315
Basic information of circular RNA
PanCircBase ID:
mmu_circCrppa_315
mm39_Chr. Position ID:
chr12|36571431|36597959|+
Gene name:
Crppa
Transcript:
ENSMUST00000221452.2
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
7,8,9
Exon/intron offsets:
0,526,26396
Genomic length:
26528
Exon/intron sizes in nt:
90,93,132
Flanking exon/introns:
chr12:36552071-36571431|chr12:36597959-36633099
Splice length:
315
Other database IDs:
circBase: mmu_circ_0003950, CIRCpedia: MMU_CIRCpedia_18123, circAtlas: mmu-Ispd_0009
circBase: hsa_circ_0133854, circAtlas: hsa-ISPD_0017, CIRCpedia: HSA_CIRCpedia_57495
Human Ortholog IDs:
Mature spliced Sequence:
TGTATGAAAATCACATCTACAGTTATGGATCACATAGGCGGAGACATTAGGAACTTCATAGAGCAATGTTACAGTTTCATCTGTGTGAATGTTGTGTCCCCTGATAGTCAAGAAACCAGGAAGTTACTGCGTATCCTCGAAGAGAGCAGCCTTCCTCTTCTGTATCCTGTAGTTGTTGTTTTGGTACACTGCTTTGACTTCACGTCAGTGCCACTCGCTCAGAAGATGGAAAGCCTGGTGTGGATTAGGGGGTTAGCAAAGGAAGTGAAAGAAAGGAATATTCTCCTAAGTGGACTCCTCCTAAACTACTCACAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GATGGAAAGCCTGGTGTGGA
59.96
AGTTCCTAATGTCTCCGCCT
58.13
146
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AGATGGAAAGCCTGGTGTGG
59.96
AGTTCCTAATGTCTCCGCCT
58.13
147
PCR template sequence:
CGCTCAGAAGATGGAAAGCCTGGTGTGGATTAGGGGGTTAGCAAAGGAAGTGAAAGAAAGGAATATTCTCCTAAGTGGACTCCTCCTAAACTACTCACAGTGTATGAAAATCACATCTACAGTTATGGATCACATAGGCGGAGACATTAGGAACTTCATAGAGCAATGTTACAGTTTCATCTGTGTGAATGTTGTGTCCC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
3
Interacting miRNAs (#binding sites)
mmu-miR-298-5p(1), mmu-miR-382-3p(1), mmu-miR-377-3p(1)
siRNAs for circular RNA silencing
Junction sequence
AAACTACTCACAGTGTATGAAAATCA
siRNA sets
GATTTTCATACACTGTGAG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCCRPPA_315_ORF_1
0.9907
0.013047548
0.761785908
627
ORF sequence:
ATGAAAATCACATCTACAGTTATGGATCACATAGGCGGAGACATTAGGAACTTCATAGAGCAATGTTACAGTTTCATCTGTGTGAATGTTGTGTCCCCTGATAGTCAAGAAACCAGGAAGTTACTGCGTATCCTCGAAGAGAGCAGCCTTCCTCTTCTGTATCCTGTAGTTGTTGTTTTGGTACACTGCTTTGACTTCACGTCAGTGCCACTCGCTCAGAAGATGGAAAGCCTGGTGTGGATTAGGGGGTTAGCAAAGGAAGTGAAAGAAAGGAATATTCTCCTAAGTGGACTCCTCCTAAACTACTCACAGTGTATGAAAATCACATCTACAGTTATGGATCACATAGGCGGAGACATTAGGAACTTCATAGAGCAATGTTACAGTTTCATCTGTGTGAATGTTGTGTCCCCTGATAGTCAAGAAACCAGGAAGTTACTGCGTATCCTCGAAGAGAGCAGCCTTCCTCTTCTGTATCCTGTAGTTGTTGTTTTGGTACACTGCTTTGACTTCACGTCAGTGCCACTCGCTCAGAAGATGGAAAGCCTGGTGTGGATTAGGGGGTTAGCAAAGGAAGTGAAAGAAAGGAATATTCTCCTAAGTGGACTCCTCCTAAACTACTCACAG
Peptide sequence:
MKITSTVMDHIGGDIRNFIEQCYSFICVNVVSPDSQETRKLLRILEESSLPLLYPVVVVLVHCFDFTSVPLAQKMESLVWIRGLAKEVKERNILLSGLLLNYSQCMKITSTVMDHIGGDIRNFIEQCYSFICVNVVSPDSQETRKLLRILEESSLPLLYPVVVVLVHCFDFTSVPLAQKMESLVWIRGLAKEVKERNILLSGLLLNYSQ
IRESfinder result
IRESfinder score
0.708403838
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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