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mmu_circCoq2_375
Basic information of circular RNA
PanCircBase ID:
mmu_circCoq2_375
mm39_Chr. Position ID:
chr5|100809702|100815924|-
Gene name:
Coq2
Transcript:
ENSMUST00000031262.9
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
4,3,2
Exon/intron offsets:
0,1743,6055
Genomic length:
6222
Exon/intron sizes in nt:
8,61,22,167
Flanking exon/introns:
chr5:100808171-100809702|chr5:100815924-100821854
Splice length:
375
Other database IDs:
circBase: hsa_circ_0070274, circAtlas: hsa-SCAF8_0001, CIRCpedia: HSA_CIRCpedia_49349
Human Ortholog IDs:
Mature spliced Sequence:
GAACCTGGCTGCTGTATTTGCCATGCACTTGGAGCATTGGTCTGGCCGCTGACCCAGGTTGTTTTCCAGACTGGTACATGTTATCACTTTTTGGCACTGGAGCTATTCTGATGCGTGGAGCAGGCTGTACTATTAATGACATGTGGGACCGTGACTTCGATAAAAAGGTTACAAGGACAGCAAACCGCCCAATCGCTGCTGGAGACATTTCGACCTTCCAGTCCTTCGTCTTCCTTGGGGGACAGCTGACCTTGGCACTGGGCGTTCTCCTGTGTTTAAACTACTACAGTATAGCAATGGGAGCAGCGTCCTTACTTCTTGTCGTCACCTACCCACTAGTGAAAAGAATCACGTTTTGGCCTCAGTTGGCTTTGG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AATGGGAGCAGCGTCCTTAC
60.11
GAAAACAACCTGGGTCAGCG
59.69
146
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CAATGGGAGCAGCGTCCTTA
60.11
GAAAACAACCTGGGTCAGCG
59.69
147
PCR template sequence:
TTAAACTACTACAGTATAGCAATGGGAGCAGCGTCCTTACTTCTTGTCGTCACCTACCCACTAGTGAAAAGAATCACGTTTTGGCCTCAGTTGGCTTTGGGAACCTGGCTGCTGTATTTGCCATGCACTTGGAGCATTGGTCTGGCCGCTGACCCAGGTTGTTTTCCAGACTGGTACATGTTATCACTTTTTGGCACTGG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
2
Interacting miRNAs (#binding sites)
mmu-miR-7a-5p(1), mmu-miR-188-3p(1)
siRNAs for circular RNA silencing
Junction sequence
CAGTTGGCTTTGGGAACCTGGCTGCT
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCCOQ2_375_ORF_1
0.8007
0.074863787
0.799602817
672
ORF sequence:
ATGTTATCACTTTTTGGCACTGGAGCTATTCTGATGCGTGGAGCAGGCTGTACTATTAATGACATGTGGGACCGTGACTTCGATAAAAAGGTTACAAGGACAGCAAACCGCCCAATCGCTGCTGGAGACATTTCGACCTTCCAGTCCTTCGTCTTCCTTGGGGGACAGCTGACCTTGGCACTGGGCGTTCTCCTGTGTTTAAACTACTACAGTATAGCAATGGGAGCAGCGTCCTTACTTCTTGTCGTCACCTACCCACTAGTGAAAAGAATCACGTTTTGGCCTCAGTTGGCTTTGGGAACCTGGCTGCTGTATTTGCCATGCACTTGGAGCATTGGTCTGGCCGCTGACCCAGGTTGTTTTCCAGACTGGTACATGTTATCACTTTTTGGCACTGGAGCTATTCTGATGCGTGGAGCAGGCTGTACTATTAATGACATGTGGGACCGTGACTTCGATAAAAAGGTTACAAGGACAGCAAACCGCCCAATCGCTGCTGGAGACATTTCGACCTTCCAGTCCTTCGTCTTCCTTGGGGGACAGCTGACCTTGGCACTGGGCGTTCTCCTGTGTTTAAACTACTACAGTATAGCAATGGGAGCAGCGTCCTTACTTCTTGTCGTCACCTACCCACTAGTGAAAAGAATCACGTTTTGGCCTCAGTTGGCTTTG
Peptide sequence:
MLSLFGTGAILMRGAGCTINDMWDRDFDKKVTRTANRPIAAGDISTFQSFVFLGGQLTLALGVLLCLNYYSIAMGAASLLLVVTYPLVKRITFWPQLALGTWLLYLPCTWSIGLAADPGCFPDWYMLSLFGTGAILMRGAGCTINDMWDRDFDKKVTRTANRPIAAGDISTFQSFVFLGGQLTLALGVLLCLNYYSIAMGAASLLLVVTYPLVKRITFWPQLAL
IRESfinder result
IRESfinder score
0.622670277
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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