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mmu_circCops7b_343
Basic information of circular RNA
PanCircBase ID:
mmu_circCops7b_343
mm39_Chr. Position ID:
chr1|86516885|86524561|+
Gene name:
Cops7b
Transcript:
ENSMUST00000121534.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
3,4,5
Exon/intron offsets:
0,3127,7587
Genomic length:
7676
Exon/intron sizes in nt:
178,76,89
Flanking exon/introns:
chr1:86514957-86516885|chr1:86524561-86526724
Splice length:
343
Other database IDs:
CIRCpedia: MMU_CIRCpedia_214076, circAtlas: mmu-Cops7b_0004
Human Ortholog IDs:
Mature spliced Sequence:
ATCTCAAGACCAGAGAATGGCAGGTGAACAGAAACCCTCAAGTAACCTCTTGGAGCAGTTCATTTTATTAGCCAAAGGTACCAGTGGCTCAGCCCTCACCACTCTCATAAGCCAGGTGCTAGAGGCTCCTGGAGTTTATGTTTTTGGAGAACTGCTGGAGTTGGCCAATGTTCAGGAGCTTGCAGAAGGAGCTAATGCTGCGTATTTGCAGTTGCTGAACCTGTTTGCCTATGGTACATACCCGGATTACATAGCCAACAAGGAGAGCCTGCCAGAACTGAGTGTGGCTCAGCAGAACAAGCTGAAGCATCTTACCATCGTGAGCTTGGCATCAAGAATGAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GAGAGCCTGCCAGAACTGAG
60.11
GCTGAGCCACTGGTACCTTT
59.96
174
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AGTGTGGCTCAGCAGAACAA
59.82
GCTGAGCCACTGGTACCTTT
59.96
156
PCR template sequence:
GGATTACATAGCCAACAAGGAGAGCCTGCCAGAACTGAGTGTGGCTCAGCAGAACAAGCTGAAGCATCTTACCATCGTGAGCTTGGCATCAAGAATGAAGATCTCAAGACCAGAGAATGGCAGGTGAACAGAAACCCTCAAGTAACCTCTTGGAGCAGTTCATTTTATTAGCCAAAGGTACCAGTGGCTCAGCCCTCACC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
NA
Interacting miRNAs (#binding sites)
NA
siRNAs for circular RNA silencing
Junction sequence
ATCAAGAATGAAGATCTCAAGACCAG
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCCOPS7B_343_ORF_1
1.0508
0.24950066
0.567004485
354
ORF sequence:
ATGGCAGGTGAACAGAAACCCTCAAGTAACCTCTTGGAGCAGTTCATTTTATTAGCCAAAGGTACCAGTGGCTCAGCCCTCACCACTCTCATAAGCCAGGTGCTAGAGGCTCCTGGAGTTTATGTTTTTGGAGAACTGCTGGAGTTGGCCAATGTTCAGGAGCTTGCAGAAGGAGCTAATGCTGCGTATTTGCAGTTGCTGAACCTGTTTGCCTATGGTACATACCCGGATTACATAGCCAACAAGGAGAGCCTGCCAGAACTGAGTGTGGCTCAGCAGAACAAGCTGAAGCATCTTACCATCGTGAGCTTGGCATCAAGAATGAAGATCTCAAGACCAGAGAATGGCAGGTGA
Peptide sequence:
MAGEQKPSSNLLEQFILLAKGTSGSALTTLISQVLEAPGVYVFGELLELANVQELAEGANAAYLQLLNLFAYGTYPDYIANKESLPELSVAQQNKLKHLTIVSLASRMKISRPENGR
IRESfinder result
IRESfinder score
0.589937034
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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