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mmu_circCkap5_3724
Basic information of circular RNA
PanCircBase ID:
mmu_circCkap5_3724
mm39_Chr. Position ID:
chr2|91376666|91425177|+
Gene name:
Ckap5
Transcript:
ENSMUST00000111338.10
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
28
Exon/intron indexes:
2,3,4,5,6,7,8,9,10,11,12,13,14,15,16,17,18,19,20,21,22,23,24,25,26,27,28,29
Exon/intron offsets:
0,2452,4230,8945,10178,11253,12727,14485,16432,16627,17998,19299,21815,22315,23858,26026,26651,29694,29841,31242,31713,33101,34788,39400,39916,42347,45344,48361
Genomic length:
48511
Exon/intron sizes in nt:
9,41,94,20,71,72,13,31,00,00,00,00,00,00,00,00,00,00,00,00,00,00,00,00,00,00,00,00,00,00,00,00,00,00,00,00,00,00,000
Flanking exon/introns:
chr2:91357195-91376666|chr2:91425177-91425655
Splice length:
3724
Other database IDs:
circAtlas: mmu-Ckap5_0125
Human Ortholog IDs:
Mature spliced Sequence:
GAACTACTTTTAACTCCAAGAGAAAACTAGAAGCACAATGGGAGATGACAGTGAGTGGTTGAAATTACCAGTTGATCAGAAATGTGAACACAAGCTATGGAAAGCAAGATTAAGTGGGTATGAAGAAGCCTTGAAGATCTTCCAGAAAATAAAGGATGAAAAGAGTCCAGAATGGTCCAAATACTTAGGATTAATCAAAAAATTTGTCACTGATTCCAATGCAGTGGTTCAATTGAAAGGACTAGAAGCTGCACTTGTCTATGTTGAAAATGCTCATGTTGCAGGAAAAACAACGGGAGAGGTTGTATCAGGTGTTGTAAGTAAAGTCTTCAACCAACCTAAAGCCAAAGCCAAGGAACTGGGCATAGAGATTTGCCTCATGTATGTTGAGATTGAAAAAGGAGAGTCTGTACAAGAAGAGCTTCTGAAGGGCCTGGATAACAAGAACCCCAAGATCATAGTGGCCTGTATAGAAACACTGAGGAAAGCCTTAAGTGAATTTGGTTCCAAAATCATCTCACTTAAGCCAATTATTAAAGTGTTGCCAAAACTATTTGAATCTCGAGACAAGGCTGTTCGGGATGAAGCCAAATTATTTGCTATAGAGATTTATCGATGGAATCGAGATGCTGTGAAACACACACTACAAAATATAAATTCTGTCCAGTTGAAAGAATTAGAAGAAGAGTGGGTCAAGCTGCCAACAGGTGCTCCTAAGCCCAGTCGGTTTCTGCGTTCCCAACAAGAACTAGAAGCTAAACTGGAACAGCAACAGTCTGCTGGTGGAGATGCAGAAGGGGGTGGTGATGATGGTGATGAGGTACCACAGGTAGATGCTTATGAGCTTCTGGATGCGGTAGAGATCCTTTCCAAACTTCCCAAAGACTTTTATGACAAAATTGAGGCAAAGAAATGGCAAGAGAGAAAAGAAGCTCTGGAGGCTGTAGAAGTACTAGTGAAAAACCCCAAGCTGGAAGCAGGCGATTACGCAGATTTAGTGAAAGCATTAAAAAAGGTTGTTGGAAAGGACACCAATGTCATGTTGGTAGCTTTGGCAGCAAAATGTCTTACTGGCTTAGCTGTTGGGCTCAGAAAGAAATTTGGACAATATGCAGGACATGTTGTGCCAACAATTTTGGAAAAATTCAAAGAAAAGAAGCCTCAAGTGGTACAAGCCCTGCAGGAGGCAATTGATGCAATCTTCCTCACTACCACACTACAGAACATCAGTGAGGATGTCTTAGCAGTAATGGATAATAAAAATCCCACCATCAAGCAGCAGACATCACTTTTTATTGCAAGAAGCTTTCGTCACTGCACTTCCTCTACATTGCCGAAGAGTCTACTAAAACCCTTTTGTGCTGCTCTACTTAAGCACATCAATGATTCTGCTCCTGAAGTCAGAGATGCTGCATTTGAAGCACTGGGTACAGCTTTGAAGGTGGTTGGTGAGAAATCAGTAAACCCATTTTTAGCTGATGTTGACAAACTCAAACTTGATAGGATCAAAGAATGTTCAGAAAAAGTAGAACTGGTTCATGGTAAAAAATCTGGACTTGCAACTGAAAAGAAGGAATCCAAGCCTCTACCTGGAAGGGCTGCAGCATCAGGGGCTGCAGGGGACAAAGACACAAAAGATGTTTCAGGACCCAAACCAGGACCGTTAAAAAAAACACCTACTGCTAAGGCTGGTGGACCATCTAAGAAAGGTAAAACAACTGCACCAGGTGGCTCAGCAAGTGCCGGGACAAAGAATAAGAAAGGATTGGAGACCAAAGAAATAGTGGAGCCAGAACTCTCAATTGAAGTATGTGAAGAGAAAGCTTCAGCTGTTCTTCCCCCAACCTGTATTCAGCTTCTAGACAGCAGCAACTGGAAGGAAAGGCTGGCCTGTATGGAAGAATTCCAAAAGGCTGTTGAACTAATGGAGCGAACTGAGATGCCGTGCCAGGCATTGGTGAAGATGCTAGCAAAAAAACCTGGATGGAAAGAAACTAATTTTCAGGTGATGCAGATGAAGCTTCACATTGTTGCTTTGATTGCCCAGAAGGGAAATTTTTCTAAAACGTCTGCTCAGATTGTATTAGATGGCCTTGTAGACAAGATTGGAGATGTGAAATGTGGGAACAATGCAAAAGAAGCCATGACAGCAATAGCTGAAGCGTGCATGCTGCCCTGGACAGCTGAGCAGGTGATGTCAATGGCCTTCTCACAAAAGAATCCTAAGAATCAATCAGAAACTCTAAATTGGCTATCAAATGCAATAAAAGAATTTGGATTTTCTGAGTTGAATGTCAAAGCTTTCATTAGCAACGTGAAGACTGCTCTTGCTGCAACAAATCCTGCTGTGAGGACTTCTGCCATCACCCTGCTTGGCGTGATGTATCTATATGTTGGTCCTTCTTTGAGAATGATCTTTGAGGATGAGAAGCCTGCCCTCCTATCCCAGATAGATGCAGAATTTCAAAAGATGCAGGGACAAAGTCCTCCCGCTCCAACGAGGGGAATTGCTAAGCACAGCACAAGTGCTACAGATGAAGGAGAAGATGGAGAGGAACCAGGTGAAGGGGGCAATGATGTTGTTGATCTTTTGCCGAGGATTGAGATCAGTGACAAAATTACTTCAGAGTTGGTGTCTAAGATTGGTGACAAGAATTGGAAGATCAGGAAAGAAGGCCTAGATGAAGTGGCAGGTATTATCAATGAAGCAAAATTTATCCAACCAAATATAGGTGAACTTCCAACTGCCTTGAAGGGTCGACTGAATGATTCAAACAAAATCTTGGTGCAGCAGACACTGAATATCCTTCAGCAACTGGCAGTAGCCATGGGTGCAAACATCAGGCAGCATGTAAAGAACCTGGGCATCCCTGTCATTACTGTTCTCGGAGATAGCAAGAACAATGTTCGAGCTGCTGCCCTAGCAACTGTGAATGCTTGGGCAGAACAAACTGGCATGAAAGAATGGCTGGAGGGAGAAGATCTTTCTGAAGAACTTAAAAAGGAAAATCCCTTCCTGAGGCAAGAGCTTCTGGGATGGCTGGCAGAGAAACTACCTACACTCCGTTCCACTCCTACAGATCTTATCCTTTGTGTTCCTCATCTGTATTCCTGTCTAGAAGATCGAAATGGAGATGTACGGAAGAAGGCCCAAGATGCTTTGCCATTCTTTATGATGCATTTAGGATATGAAAAAATGGCTAAGGCTACTGGGAAACTAAAGCCAACTTCTAAAGATCAAGTACTGGCCATGCTAGAAAAGGCCAAAGCTAACATGCCATCCAAGCCTGCTGCACCTGCTAAGGCCATGTCCAAACCAATGGGAGGGTCGGCTCCAGCCAAAACCCAGCCTATACCAGCACCTGTTGAAGATTCTGTTTCCAGTACCATAGAAGCTAAGCCTGATCTGAAAAAGGCTAAAGCTCCAGGCGTTTCTTCCAAAGCAAAGAGTGTACAAGGGAAGAAAGTGCCAAGCAAAACCACCTTAAAGGAAGACGACGACAAATCTGGTCCTATTTTTATTGTTGTTCCAAATGGAAAGGAGCAAAGGATGAGAGATGAGAAAGGATTAAAGGTGCTAAAGTGGAATTTTACTACCCCACGGGATGAGTACATTGAACAGCTGAAGACTCAGATGTCTACCTGTGTGGCTAAATGGTTACAGGATGAAATGTTTCACTCAGATTTCCAGCATCACAATAAAGCCCTAGCTGTCATGGTTGAT
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TCAGATGTCTACCTGTGTGGC
59.45
CCACTCACTGTCATCTCCCA
58.73
152
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TCAGATGTCTACCTGTGTGGC
59.45
ACCACTCACTGTCATCTCCC
58.73
153
PCR template sequence:
GAAGACTCAGATGTCTACCTGTGTGGCTAAATGGTTACAGGATGAAATGTTTCACTCAGATTTCCAGCATCACAATAAAGCCCTAGCTGTCATGGTTGATGAACTACTTTTAACTCCAAGAGAAAACTAGAAGCACAATGGGAGATGACAGTGAGTGGTTGAAATTACCAGTTGATCAGAAATGTGAACACAAGCTATGG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
29
Interacting miRNAs (#binding sites)
mmu-miR-33-5p(2), mmu-miR-136-5p(2), mmu-miR-9-5p(1), mmu-miR-16-5p(1), mmu-miR-15a-5p(1), mmu-miR-27b-3p(1), mmu-miR-27a-3p(1), mmu-miR-181a-5p(1), mmu-miR-103-3p(1), mmu-miR-135a-5p(1), mmu-miR-15b-5p(1), mmu-miR-216a-5p(1), mmu-miR-217-5p(1), mmu-miR-196a-5p(1), mmu-miR-214-3p(1), mmu-miR-149-5p(1), mmu-miR-434-3p(1), mmu-miR-494-3p(1), mmu-miR-96-5p(1), mmu-miR-483-5p(1), mmu-miR-107-3p(1), mmu-miR-27a-5p(1), mmu-miR-29b-2-5p(1), mmu-miR-207(1), mmu-miR-673-5p(1), mmu-miR-369-3p(1), mmu-miR-335-3p(1), mmu-miR-669d-5p(1), mmu-miR-3087-3p(1)
siRNAs for circular RNA silencing
Junction sequence
TGTCATGGTTGATGAACTACTTTTAA
siRNA sets
AAAAGTAGTTCATCAACCA, TTAAAAGTAGTTCATCAAC, AAAGTAGTTCATCAACCAT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCCKAP5_3724_ORF_1
1.095
0.137793454
1
3717
ORF sequence:
ATGGGAGATGACAGTGAGTGGTTGAAATTACCAGTTGATCAGAAATGTGAACACAAGCTATGGAAAGCAAGATTAAGTGGGTATGAAGAAGCCTTGAAGATCTTCCAGAAAATAAAGGATGAAAAGAGTCCAGAATGGTCCAAATACTTAGGATTAATCAAAAAATTTGTCACTGATTCCAATGCAGTGGTTCAATTGAAAGGACTAGAAGCTGCACTTGTCTATGTTGAAAATGCTCATGTTGCAGGAAAAACAACGGGAGAGGTTGTATCAGGTGTTGTAAGTAAAGTCTTCAACCAACCTAAAGCCAAAGCCAAGGAACTGGGCATAGAGATTTGCCTCATGTATGTTGAGATTGAAAAAGGAGAGTCTGTACAAGAAGAGCTTCTGAAGGGCCTGGATAACAAGAACCCCAAGATCATAGTGGCCTGTATAGAAACACTGAGGAAAGCCTTAAGTGAATTTGGTTCCAAAATCATCTCACTTAAGCCAATTATTAAAGTGTTGCCAAAACTATTTGAATCTCGAGACAAGGCTGTTCGGGATGAAGCCAAATTATTTGCTATAGAGATTTATCGATGGAATCGAGATGCTGTGAAACACACACTACAAAATATAAATTCTGTCCAGTTGAAAGAATTAGAAGAAGAGTGGGTCAAGCTGCCAACAGGTGCTCCTAAGCCCAGTCGGTTTCTGCGTTCCCAACAAGAACTAGAAGCTAAACTGGAACAGCAACAGTCTGCTGGTGGAGATGCAGAAGGGGGTGGTGATGATGGTGATGAGGTACCACAGGTAGATGCTTATGAGCTTCTGGATGCGGTAGAGATCCTTTCCAAACTTCCCAAAGACTTTTATGACAAAATTGAGGCAAAGAAATGGCAAGAGAGAAAAGAAGCTCTGGAGGCTGTAGAAGTACTAGTGAAAAACCCCAAGCTGGAAGCAGGCGATTACGCAGATTTAGTGAAAGCATTAAAAAAGGTTGTTGGAAAGGACACCAATGTCATGTTGGTAGCTTTGGCAGCAAAATGTCTTACTGGCTTAGCTGTTGGGCTCAGAAAGAAATTTGGACAATATGCAGGACATGTTGTGCCAACAATTTTGGAAAAATTCAAAGAAAAGAAGCCTCAAGTGGTACAAGCCCTGCAGGAGGCAATTGATGCAATCTTCCTCACTACCACACTACAGAACATCAGTGAGGATGTCTTAGCAGTAATGGATAATAAAAATCCCACCATCAAGCAGCAGACATCACTTTTTATTGCAAGAAGCTTTCGTCACTGCACTTCCTCTACATTGCCGAAGAGTCTACTAAAACCCTTTTGTGCTGCTCTACTTAAGCACATCAATGATTCTGCTCCTGAAGTCAGAGATGCTGCATTTGAAGCACTGGGTACAGCTTTGAAGGTGGTTGGTGAGAAATCAGTAAACCCATTTTTAGCTGATGTTGACAAACTCAAACTTGATAGGATCAAAGAATGTTCAGAAAAAGTAGAACTGGTTCATGGTAAAAAATCTGGACTTGCAACTGAAAAGAAGGAATCCAAGCCTCTACCTGGAAGGGCTGCAGCATCAGGGGCTGCAGGGGACAAAGACACAAAAGATGTTTCAGGACCCAAACCAGGACCGTTAAAAAAAACACCTACTGCTAAGGCTGGTGGACCATCTAAGAAAGGTAAAACAACTGCACCAGGTGGCTCAGCAAGTGCCGGGACAAAGAATAAGAAAGGATTGGAGACCAAAGAAATAGTGGAGCCAGAACTCTCAATTGAAGTATGTGAAGAGAAAGCTTCAGCTGTTCTTCCCCCAACCTGTATTCAGCTTCTAGACAGCAGCAACTGGAAGGAAAGGCTGGCCTGTATGGAAGAATTCCAAAAGGCTGTTGAACTAATGGAGCGAACTGAGATGCCGTGCCAGGCATTGGTGAAGATGCTAGCAAAAAAACCTGGATGGAAAGAAACTAATTTTCAGGTGATGCAGATGAAGCTTCACATTGTTGCTTTGATTGCCCAGAAGGGAAATTTTTCTAAAACGTCTGCTCAGATTGTATTAGATGGCCTTGTAGACAAGATTGGAGATGTGAAATGTGGGAACAATGCAAAAGAAGCCATGACAGCAATAGCTGAAGCGTGCATGCTGCCCTGGACAGCTGAGCAGGTGATGTCAATGGCCTTCTCACAAAAGAATCCTAAGAATCAATCAGAAACTCTAAATTGGCTATCAAATGCAATAAAAGAATTTGGATTTTCTGAGTTGAATGTCAAAGCTTTCATTAGCAACGTGAAGACTGCTCTTGCTGCAACAAATCCTGCTGTGAGGACTTCTGCCATCACCCTGCTTGGCGTGATGTATCTATATGTTGGTCCTTCTTTGAGAATGATCTTTGAGGATGAGAAGCCTGCCCTCCTATCCCAGATAGATGCAGAATTTCAAAAGATGCAGGGACAAAGTCCTCCCGCTCCAACGAGGGGAATTGCTAAGCACAGCACAAGTGCTACAGATGAAGGAGAAGATGGAGAGGAACCAGGTGAAGGGGGCAATGATGTTGTTGATCTTTTGCCGAGGATTGAGATCAGTGACAAAATTACTTCAGAGTTGGTGTCTAAGATTGGTGACAAGAATTGGAAGATCAGGAAAGAAGGCCTAGATGAAGTGGCAGGTATTATCAATGAAGCAAAATTTATCCAACCAAATATAGGTGAACTTCCAACTGCCTTGAAGGGTCGACTGAATGATTCAAACAAAATCTTGGTGCAGCAGACACTGAATATCCTTCAGCAACTGGCAGTAGCCATGGGTGCAAACATCAGGCAGCATGTAAAGAACCTGGGCATCCCTGTCATTACTGTTCTCGGAGATAGCAAGAACAATGTTCGAGCTGCTGCCCTAGCAACTGTGAATGCTTGGGCAGAACAAACTGGCATGAAAGAATGGCTGGAGGGAGAAGATCTTTCTGAAGAACTTAAAAAGGAAAATCCCTTCCTGAGGCAAGAGCTTCTGGGATGGCTGGCAGAGAAACTACCTACACTCCGTTCCACTCCTACAGATCTTATCCTTTGTGTTCCTCATCTGTATTCCTGTCTAGAAGATCGAAATGGAGATGTACGGAAGAAGGCCCAAGATGCTTTGCCATTCTTTATGATGCATTTAGGATATGAAAAAATGGCTAAGGCTACTGGGAAACTAAAGCCAACTTCTAAAGATCAAGTACTGGCCATGCTAGAAAAGGCCAAAGCTAACATGCCATCCAAGCCTGCTGCACCTGCTAAGGCCATGTCCAAACCAATGGGAGGGTCGGCTCCAGCCAAAACCCAGCCTATACCAGCACCTGTTGAAGATTCTGTTTCCAGTACCATAGAAGCTAAGCCTGATCTGAAAAAGGCTAAAGCTCCAGGCGTTTCTTCCAAAGCAAAGAGTGTACAAGGGAAGAAAGTGCCAAGCAAAACCACCTTAAAGGAAGACGACGACAAATCTGGTCCTATTTTTATTGTTGTTCCAAATGGAAAGGAGCAAAGGATGAGAGATGAGAAAGGATTAAAGGTGCTAAAGTGGAATTTTACTACCCCACGGGATGAGTACATTGAACAGCTGAAGACTCAGATGTCTACCTGTGTGGCTAAATGGTTACAGGATGAAATGTTTCACTCAGATTTCCAGCATCACAATAAAGCCCTAGCTGTCATGGTTGATGAACTACTTTTAACTCCAAGAGAAAACTAG
Peptide sequence:
MGDDSEWLKLPVDQKCEHKLWKARLSGYEEALKIFQKIKDEKSPEWSKYLGLIKKFVTDSNAVVQLKGLEAALVYVENAHVAGKTTGEVVSGVVSKVFNQPKAKAKELGIEICLMYVEIEKGESVQEELLKGLDNKNPKIIVACIETLRKALSEFGSKIISLKPIIKVLPKLFESRDKAVRDEAKLFAIEIYRWNRDAVKHTLQNINSVQLKELEEEWVKLPTGAPKPSRFLRSQQELEAKLEQQQSAGGDAEGGGDDGDEVPQVDAYELLDAVEILSKLPKDFYDKIEAKKWQERKEALEAVEVLVKNPKLEAGDYADLVKALKKVVGKDTNVMLVALAAKCLTGLAVGLRKKFGQYAGHVVPTILEKFKEKKPQVVQALQEAIDAIFLTTTLQNISEDVLAVMDNKNPTIKQQTSLFIARSFRHCTSSTLPKSLLKPFCAALLKHINDSAPEVRDAAFEALGTALKVVGEKSVNPFLADVDKLKLDRIKECSEKVELVHGKKSGLATEKKESKPLPGRAAASGAAGDKDTKDVSGPKPGPLKKTPTAKAGGPSKKGKTTAPGGSASAGTKNKKGLETKEIVEPELSIEVCEEKASAVLPPTCIQLLDSSNWKERLACMEEFQKAVELMERTEMPCQALVKMLAKKPGWKETNFQVMQMKLHIVALIAQKGNFSKTSAQIVLDGLVDKIGDVKCGNNAKEAMTAIAEACMLPWTAEQVMSMAFSQKNPKNQSETLNWLSNAIKEFGFSELNVKAFISNVKTALAATNPAVRTSAITLLGVMYLYVGPSLRMIFEDEKPALLSQIDAEFQKMQGQSPPAPTRGIAKHSTSATDEGEDGEEPGEGGNDVVDLLPRIEISDKITSELVSKIGDKNWKIRKEGLDEVAGIINEAKFIQPNIGELPTALKGRLNDSNKILVQQTLNILQQLAVAMGANIRQHVKNLGIPVITVLGDSKNNVRAAALATVNAWAEQTGMKEWLEGEDLSEELKKENPFLRQELLGWLAEKLPTLRSTPTDLILCVPHLYSCLEDRNGDVRKKAQDALPFFMMHLGYEKMAKATGKLKPTSKDQVLAMLEKAKANMPSKPAAPAKAMSKPMGGSAPAKTQPIPAPVEDSVSSTIEAKPDLKKAKAPGVSSKAKSVQGKKVPSKTTLKEDDDKSGPIFIVVPNGKEQRMRDEKGLKVLKWNFTTPRDEYIEQLKTQMSTCVAKWLQDEMFHSDFQHHNKALAVMVDELLLTPREN
IRESfinder result
IRESfinder score
0.475407434
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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