top of page
mmu_circCiz1_2163
Basic information of circular RNA
PanCircBase ID:
mmu_circCiz1_2163
mm39_Chr. Position ID:
chr2|32261647|32268119|+
Gene name:
Ciz1
Transcript:
ENSMUST00000192758.6
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
8
Exon/intron indexes:
10,11,12,13,14,15,16,17
Exon/intron offsets:
0,662,2706,4373,4630,5657,5873,6328
Genomic length:
6472
Exon/intron sizes in nt:
1,22,19,81,23,11,14,16,00,00,00,000
Flanking exon/introns:
chr2:32261420-32261647|chr2:32268119-32269938
Splice length:
2163
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
ACCCAGACAAGGCCTTGCCAGAACCAGTAAGTGCCCAGAGCAGTGAAGACAGGAGCCGGGAGGCGTCCGCTGGTGGCCTGGATTTGGGAGAATGTGAAAAGAGAGCGGGAGAGATGCTGGGGATGTGGGGGGCTGGGAGCTCCCTGAAGGTCACCATCCTGCAGAGTAGCAACAGCCGGGCCTTTAACACCACACCCCTCACATCTGGACCTCGCCCTGGGGACTCTACCTCTGCCACCCCTGCCATTGCCAGCACACCCTCCAAGCAAAGCCTCCAGTTCTTCTGCTACATCTGCAAGGCCAGCAGCAGCAGCCAGCAGGAGTTCCAGGATCACATGTCAGAGGCTCAGCACCAACAGCGGCTTGGGGAAATACAACACTCGAGCCAGACCTGCCTGCTGTCCCTGCTGCCCATGCCTCGGGACATCCTGGAGAAAGAAGCGGAGTAAGTAGGGGCTGGCAGAGGCTCATGTGGGTCAGGCAGCGTCCCAGGCCAGGCCCTTAATAGACTGGCTGGCTGGGAGGTCAACCAGCCGTGCATACCATTAACTCCAATTTTGATCATCAGTTTCTCCTGCCTGTAAAATGTCTTGGTGAGGGGTGTAGTGACAGTCTCCTAAGGTCCCTTCCAGGTAAGAACAGTGTGACATTCCTATGGAAGAGAGGGGAGCTGTAATACAGAGAGACCTACCCCCACTGATCTGTGTGCTAGCCCTAACCAGAGAGGCACAGCGGTAAGGACCAGGTCATCTTGTGGCCCTGGGTGAGACCTTCCCTGAATGTATCCATTGTCACAGGGGATGTGGTTGGTCTGAAATGTCTACCCAGAATCTGGAACTAGCACCTGGTGTGAGAAGGCTTGGTGGGAAGTTGTAGGTCCCAGTGGGGTGTCCCAAGCCTGCAGGGCTGGAAAGCATCACTGGAGAGTGTCAAGCTGAGACTTGGGCCCTGGGAGACAAGGGCTGTGTCTCCTGGGGGAGCTGCCTCCGGTGGTTGTTAGAGCTCCCAGCTTTCCAGGGGGATCCAGGCACCTGGATAACTTCGGAAGCCTCACAGTTGCAGAGCATCATCAACAAAGTCAGATGTGATCCCGGCACTTGGGAGGCTGAGACAGGGCACAAGAGTTGACTTATTCAGAATGACAGAAAACCCAGATGAAGACAAATGAAACCGCTTCTCAGGCGGTACTGTTGTACGAGTCCATGATGGGAGGTTCCACTGAGGGCAAGGACAGGGACTCTTGGCTGCTCTGTGATTCACCTGGCTCCATCCGTTAGCTCCCACTTATAGAAGAGTCAGGAATCGCTATCCTGTCCTTTGTCTTTTTGATGCTCTGACTAGGACCCTGGCCCCTCATATACCCTAGCTTCTGAAGTGCAGCACCCTTCCCTTCCCTTCCCTTCCCTTCCGGAGCACTCTAGGAGAATATAGCATCACACGCTGCTTGCTTTTATTGCCGTCAGAGATCCTCCGCCCAAACGCTGGTGCAACACCTGCCAGGTGTACTACGTGGGAGACTTGATCCAGCACCGTAGGACACAGGAGCACAAGGTTGCCAAACAATCCCTGAGGCCCTTCTGCACCATATGCAACCGTTACTTCAAGACCCCTCGAAAGTTTGTGGAGCACGTGAAGTCCCAGGGACACAAGGACAAGGCCCAAGAGCTGAAGACACTTGAAAAGGAGACAGGCAGCCCAGATGAGGACCACTTCATCACTGTGGACGCCGTCGGTTGCTTTGAGAGTGGTCAAGAAGAGGACGAGGATGACGACGAGGAAGAAGAAGAAGAAGGAGAGATTGAGGCTGAGGAGGAATTCTGCAAGCAGGTGAAGCCGAGAGAAACATCCTCAGAGCAAGGGAAGGGCTCTGAGACGTACAACCCCAACACAGCCTATGGTGAGGATTTCCTGGTGCCAGTGATGGGCTATGTCTGTCAAATCTGTCACAAGTTCTACGACAGCAACTCAGAATTGCGGCTTTCTCACTGCAAGTCCCTGGCCCACTTTGAGAACCTGCAGAAATACAAAGCCAAGAACCCAAGCCCTCCTCCTACCCGGCCTGTGAGCCGCAAGTGTGCCATCAACGCCCGCAACGCCCTGACTGCACTGTTCACCTCTAGCCACCAGCCCAGCCCCCAGGACACAGTGAAAATGCCCAGCAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CCTGACTGCACTGTTCACCT
59.89
TTCTCCCAAATCCAGGCCAC
59.96
159
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GCACTGTTCACCTCTAGCCA
59.68
TTCTCCCAAATCCAGGCCAC
59.96
152
PCR template sequence:
GAGCCGCAAGTGTGCCATCAACGCCCGCAACGCCCTGACTGCACTGTTCACCTCTAGCCACCAGCCCAGCCCCCAGGACACAGTGAAAATGCCCAGCAAGACCCAGACAAGGCCTTGCCAGAACCAGTAAGTGCCCAGAGCAGTGAAGACAGGAGCCGGGAGGCGTCCGCTGGTGGCCTGGATTTGGGAGAATGTGAAAA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
16
Interacting miRNAs (#binding sites)
mmu-miR-149-5p(2), mmu-miR-705(2), mmu-miR-150-5p(1), mmu-miR-101a-3p(1), mmu-miR-122-5p(1), mmu-let-7c-5p(1), mmu-miR-148a-3p(1), mmu-miR-298-5p(1), mmu-miR-320-3p(1), mmu-miR-324-5p(1), mmu-miR-326-3p(1), mmu-miR-339-5p(1), mmu-miR-214-3p(1), mmu-miR-96-5p(1), mmu-miR-182-5p(1), mmu-miR-3061-5p(1)
siRNAs for circular RNA silencing
Junction sequence
AATGCCCAGCAAGACCCAGACAAGGC
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCCIZ1_2163_ORF_3
0.9353
0.386031231
0.485872986
273
ORF sequence:
ATGGGCTATGTCTGTCAAATCTGTCACAAGTTCTACGACAGCAACTCAGAATTGCGGCTTTCTCACTGCAAGTCCCTGGCCCACTTTGAGAACCTGCAGAAATACAAAGCCAAGAACCCAAGCCCTCCTCCTACCCGGCCTGTGAGCCGCAAGTGTGCCATCAACGCCCGCAACGCCCTGACTGCACTGTTCACCTCTAGCCACCAGCCCAGCCCCCAGGACACAGTGAAAATGCCCAGCAAGACCCAGACAAGGCCTTGCCAGAACCAGTAA
Peptide sequence:
MGYVCQICHKFYDSNSELRLSHCKSLAHFENLQKYKAKNPSPPPTRPVSRKCAINARNALTALFTSSHQPSPQDTVKMPSKTQTRPCQNQ
IRESfinder result
IRESfinder score
0.433944139
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
bottom of page