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mmu_circCfap61_1362
Basic information of circular RNA
PanCircBase ID:
mmu_circCfap61_1362
mm39_Chr. Position ID:
chr2|145937543|145995837|+
Gene name:
Cfap61
Transcript:
ENSMUST00000133433.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
7
Exon/intron indexes:
19,20,21,22,23,24,25
Exon/intron offsets:
0,6329,14283,33330,47389,49760,58088
Genomic length:
58294
Exon/intron sizes in nt:
99,16,91,75,29,33,28,90,000
Flanking exon/introns:
chr2:145919308-145937543|chr2:145995837-146042629
Splice length:
1362
Other database IDs:
circAtlas: hsa-SCAF8_0001
Human Ortholog IDs:
Mature spliced Sequence:
CTCTCACCTGAAATTTAATAATCTCACCCTGATTTCAACTCATGGACTCCCAGGAAAAAGACTTTTGCACAACGAACAGAGGAAGTTTCTAGCAAGCGACCACTGTTTTAATGATAAGGATTATGCACTGATGTCACTGTGCTCCTGGGTGAACGTTGTGGTTGGTCGAATGACGGCCATAGACCGAGCAGCCAAGCAGGTTGTGGTTTCCAAGAGTGAGATCGTGTTCTACGACCACCTCATTCTCTGTACCGGGCTGCAGTACCAGGTTCCGTGCCCCACAGGGGCTGATACTGAGCAGCACCTGACGAACAGAGAGGTTTTGGAGCTCAGCAAGCAGCGGTACACCGGCACTGTTCCTTCCAACCTGTTCATTCTCAACGACGAGGAAGACTGCGTTAGGATGCTCTGTTGGCTGAGAAACAGCTCCATCCTCACAGAAGGGAAGGTCATTGTCTACGGGAATACACTCGACGCTTACACAACAGTGGAGACACTCCTCAACATTGGCATGAAGGGGAGCGGCATCTACTTTGTGCATCCCCCAACTGAATCAAACATAACCTGTATCAACAACAATGATGTAGAGAGTGCCGTGGAAGATGCACTCAGTGCCGCTGGGGTGACAGTCTTTCATGACGCCTTGTTGGCTCAGTGGAACCATGGCCAGCACCCGGATCCTATCTACAATGCCTGCTTCACCACCTCCACCAAGCCTATCAGACTGGAGTGTTCAGCGTTCTTCAGCTTCTACAAGAAGAATGTGGATTACGAAACATTTAAAGCATTTAATGATGCATGCCTTGTCTATGATGGCCGACTTGTGATCGATACTACCTTCCACACCAATGACATAGCCATCAGAGCTGCTGGTTCCCTCACCAAATTCTCCAACCGATATTACTCAAATGAGTGGACTCACAGCAATTTCAGTTCCAAAGAAATTGGCTTCCAGCTGGCTGCAGCTATGCTCAGTCTCTTTGACCCAACCCTTGAGCCTGTGACTGAACCACCAGCTGACCTTGACCGCCTCATCCCCATGTACAAGGGAGCCAAGATCCAAGGAGGGATTCTTCCTGGGTCTTATCATTATCTGCATATTTCCAAACCTGCCATCCCAACTCCTTTGAATCTACAAAGGTCACAGCCTGATTTTGGTTCAGACGTAGTGACAGGTAATGCGAAAAAGGGGACGTACTTCCGACTATACATTAACAACTATAAGTTGGTGGAGGCCATCACGTGCTTTTCAAAGGAGCCTTTCCCATCCTCCAACTACATCCGCTTGTTTGGACAACATGAGCAAGTCCTCAACAACCTGTGTACTCGCTTTGATGACAAACTGATCCCAGATCTTTACAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CCTCCAACTACATCCGCTTGT
60.07
ACTTCCTCTGTTCGTTGTGCA
60.13
180
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CCTCCAACTACATCCGCTTGT
60.07
TCCTGGGAGTCCATGAGTTGA
60.2
149
PCR template sequence:
TCCCATCCTCCAACTACATCCGCTTGTTTGGACAACATGAGCAAGTCCTCAACAACCTGTGTACTCGCTTTGATGACAAACTGATCCCAGATCTTTACAGCTCTCACCTGAAATTTAATAATCTCACCCTGATTTCAACTCATGGACTCCCAGGAAAAAGACTTTTGCACAACGAACAGAGGAAGTTTCTAGCAAGCGAC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
7
Interacting miRNAs (#binding sites)
mmu-miR-122-5p(1), mmu-miR-205-5p(1), mmu-miR-152-3p(1), mmu-miR-138-5p(1), mmu-miR-128-3p(1), mmu-miR-296-3p(1), mmu-miR-376a-3p(1)
siRNAs for circular RNA silencing
Junction sequence
CAGATCTTTACAGCTCTCACCTGAAA
siRNA sets
TTTCAGGTGAGAGCTGTAA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCCFAP61_1362_ORF_1
1.1137
0.291137771
0.999999942
2592
ORF sequence:
ATGTCACTGTGCTCCTGGGTGAACGTTGTGGTTGGTCGAATGACGGCCATAGACCGAGCAGCCAAGCAGGTTGTGGTTTCCAAGAGTGAGATCGTGTTCTACGACCACCTCATTCTCTGTACCGGGCTGCAGTACCAGGTTCCGTGCCCCACAGGGGCTGATACTGAGCAGCACCTGACGAACAGAGAGGTTTTGGAGCTCAGCAAGCAGCGGTACACCGGCACTGTTCCTTCCAACCTGTTCATTCTCAACGACGAGGAAGACTGCGTTAGGATGCTCTGTTGGCTGAGAAACAGCTCCATCCTCACAGAAGGGAAGGTCATTGTCTACGGGAATACACTCGACGCTTACACAACAGTGGAGACACTCCTCAACATTGGCATGAAGGGGAGCGGCATCTACTTTGTGCATCCCCCAACTGAATCAAACATAACCTGTATCAACAACAATGATGTAGAGAGTGCCGTGGAAGATGCACTCAGTGCCGCTGGGGTGACAGTCTTTCATGACGCCTTGTTGGCTCAGTGGAACCATGGCCAGCACCCGGATCCTATCTACAATGCCTGCTTCACCACCTCCACCAAGCCTATCAGACTGGAGTGTTCAGCGTTCTTCAGCTTCTACAAGAAGAATGTGGATTACGAAACATTTAAAGCATTTAATGATGCATGCCTTGTCTATGATGGCCGACTTGTGATCGATACTACCTTCCACACCAATGACATAGCCATCAGAGCTGCTGGTTCCCTCACCAAATTCTCCAACCGATATTACTCAAATGAGTGGACTCACAGCAATTTCAGTTCCAAAGAAATTGGCTTCCAGCTGGCTGCAGCTATGCTCAGTCTCTTTGACCCAACCCTTGAGCCTGTGACTGAACCACCAGCTGACCTTGACCGCCTCATCCCCATGTACAAGGGAGCCAAGATCCAAGGAGGGATTCTTCCTGGGTCTTATCATTATCTGCATATTTCCAAACCTGCCATCCCAACTCCTTTGAATCTACAAAGGTCACAGCCTGATTTTGGTTCAGACGTAGTGACAGGTAATGCGAAAAAGGGGACGTACTTCCGACTATACATTAACAACTATAAGTTGGTGGAGGCCATCACGTGCTTTTCAAAGGAGCCTTTCCCATCCTCCAACTACATCCGCTTGTTTGGACAACATGAGCAAGTCCTCAACAACCTGTGTACTCGCTTTGATGACAAACTGATCCCAGATCTTTACAGCTCTCACCTGAAATTTAATAATCTCACCCTGATTTCAACTCATGGACTCCCAGGAAAAAGACTTTTGCACAACGAACAGAGGAAGTTTCTAGCAAGCGACCACTGTTTTAATGATAAGGATTATGCACTGATGTCACTGTGCTCCTGGGTGAACGTTGTGGTTGGTCGAATGACGGCCATAGACCGAGCAGCCAAGCAGGTTGTGGTTTCCAAGAGTGAGATCGTGTTCTACGACCACCTCATTCTCTGTACCGGGCTGCAGTACCAGGTTCCGTGCCCCACAGGGGCTGATACTGAGCAGCACCTGACGAACAGAGAGGTTTTGGAGCTCAGCAAGCAGCGGTACACCGGCACTGTTCCTTCCAACCTGTTCATTCTCAACGACGAGGAAGACTGCGTTAGGATGCTCTGTTGGCTGAGAAACAGCTCCATCCTCACAGAAGGGAAGGTCATTGTCTACGGGAATACACTCGACGCTTACACAACAGTGGAGACACTCCTCAACATTGGCATGAAGGGGAGCGGCATCTACTTTGTGCATCCCCCAACTGAATCAAACATAACCTGTATCAACAACAATGATGTAGAGAGTGCCGTGGAAGATGCACTCAGTGCCGCTGGGGTGACAGTCTTTCATGACGCCTTGTTGGCTCAGTGGAACCATGGCCAGCACCCGGATCCTATCTACAATGCCTGCTTCACCACCTCCACCAAGCCTATCAGACTGGAGTGTTCAGCGTTCTTCAGCTTCTACAAGAAGAATGTGGATTACGAAACATTTAAAGCATTTAATGATGCATGCCTTGTCTATGATGGCCGACTTGTGATCGATACTACCTTCCACACCAATGACATAGCCATCAGAGCTGCTGGTTCCCTCACCAAATTCTCCAACCGATATTACTCAAATGAGTGGACTCACAGCAATTTCAGTTCCAAAGAAATTGGCTTCCAGCTGGCTGCAGCTATGCTCAGTCTCTTTGACCCAACCCTTGAGCCTGTGACTGAACCACCAGCTGACCTTGACCGCCTCATCCCCATGTACAAGGGAGCCAAGATCCAAGGAGGGATTCTTCCTGGGTCTTATCATTATCTGCATATTTCCAAACCTGCCATCCCAACTCCTTTGAATCTACAAAGGTCACAGCCTGATTTTGGTTCAGACGTAGTGACAGGTAATGCGAAAAAGGGGACGTACTTCCGACTATACATTAACAACTATAAGTTGGTGGAGGCCATCACGTGCTTTTCAAAGGAGCCTTTCCCATCCTCCAACTACATCCGCTTGTTTGGACAACATGAGCAAGTCCTCAACAACCTGTGTACTCGCTTTGATGACAAACTGATCCCAGATCTTTAC
Peptide sequence:
MSLCSWVNVVVGRMTAIDRAAKQVVVSKSEIVFYDHLILCTGLQYQVPCPTGADTEQHLTNREVLELSKQRYTGTVPSNLFILNDEEDCVRMLCWLRNSSILTEGKVIVYGNTLDAYTTVETLLNIGMKGSGIYFVHPPTESNITCINNNDVESAVEDALSAAGVTVFHDALLAQWNHGQHPDPIYNACFTTSTKPIRLECSAFFSFYKKNVDYETFKAFNDACLVYDGRLVIDTTFHTNDIAIRAAGSLTKFSNRYYSNEWTHSNFSSKEIGFQLAAAMLSLFDPTLEPVTEPPADLDRLIPMYKGAKIQGGILPGSYHYLHISKPAIPTPLNLQRSQPDFGSDVVTGNAKKGTYFRLYINNYKLVEAITCFSKEPFPSSNYIRLFGQHEQVLNNLCTRFDDKLIPDLYSSHLKFNNLTLISTHGLPGKRLLHNEQRKFLASDHCFNDKDYALMSLCSWVNVVVGRMTAIDRAAKQVVVSKSEIVFYDHLILCTGLQYQVPCPTGADTEQHLTNREVLELSKQRYTGTVPSNLFILNDEEDCVRMLCWLRNSSILTEGKVIVYGNTLDAYTTVETLLNIGMKGSGIYFVHPPTESNITCINNNDVESAVEDALSAAGVTVFHDALLAQWNHGQHPDPIYNACFTTSTKPIRLECSAFFSFYKKNVDYETFKAFNDACLVYDGRLVIDTTFHTNDIAIRAAGSLTKFSNRYYSNEWTHSNFSSKEIGFQLAAAMLSLFDPTLEPVTEPPADLDRLIPMYKGAKIQGGILPGSYHYLHISKPAIPTPLNLQRSQPDFGSDVVTGNAKKGTYFRLYINNYKLVEAITCFSKEPFPSSNYIRLFGQHEQVLNNLCTRFDDKLIPDLY
IRESfinder result
IRESfinder score
0.620236446
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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