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mmu_circCep95_1163
Basic information of circular RNA
PanCircBase ID:
mmu_circCep95_1163
mm39_Chr. Position ID:
chr11|106683302|106703361|+
Gene name:
Cep95
Transcript:
ENSMUST00000103068.10
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
9
Exon/intron indexes:
3,4,5,6,7,8,9,10,11
Exon/intron offsets:
0,3646,7567,8986,13759,16715,17151,18719,19919
Genomic length:
20059
Exon/intron sizes in nt:
10,81,11,10,01,16,19,40,00,00,00,00,000
Flanking exon/introns:
chr11:106681685-106683302|chr11:106703361-106704586
Splice length:
1163
Other database IDs:
circAtlas: hsa-SCAF8_0001, CIRCpedia: HSA_CIRCpedia_120286
Human Ortholog IDs:
Mature spliced Sequence:
ACCTCATAGTTCTTCCCAGGAATCAAGAAGACGAGGCCCACAATGTCCAAGCGGTAATTGATTCACTCGCTTTGGATTACCTTCAGGTCAGCCTGTCTCACATAACAGGAGAAAACATAGTGAAAGGGGATAATGAATCTATCAGGAATCTCCTAGAAATATTTGATGGATTGCTGGATTATCTGACAGAACACATCAGTGAATCGTCTCCCAACAAAAGTGAAACTGAGCAGTATTCTAAAGATTCTCATGGAGAAGAAGCTGGGGAGGACCTAGAACGTACCGAGGAGGCGAAGTGGAGAAACGCTTCTTTTATGAGGTGTTCCTTCTCATCGGACACTTTGGGCCCCACCTGGGATGAAGACGAAGCTGAGTCCACAGGTGAAATAATTAGGCTGGGAGACACTGCACATACCTTTTCCCAAAGAAGTAATGCATCAGAAACTCCTCCTATGAGCATGGTTCCAAGTGCCAGGAAGCTAGGCGAGCCGATCCGAGCTGCCATTCCTTTACATCCTCCCTACCATCCTTCTGAGCCTAGAGCACCATGTCCCATAGGCAAAGAATACCTCTGGTCAAGTCGCTACTTGTCTACGCCGACTTCTGGAGAGCACATGGCACCTTCTGTGGAACCAGATGATGCTTTCTTAACTTCTACCTTATTTAAAGATGATGATCAGGAAACATATCTACCTAAACCTGAAGCCACCAGGACAAGGAAGCCTTCTAAAGGAGAAAGAGATGAAAACAGAGCTGCGATCCCCTCAGAGCATCTGCCTTTCTCTCAGAAGGCCAGGAAGCCGTTAACAGAACAAGAACTGCACGCCATGTCAGAGAAACTCTCTCAGCGGCTCTCTGAACTAGACTGGATGTTAAAAACTGCCCTGGGTGATCGAGGAACGGGCGAAACTGATGGTAATGATGGTGATGGCGGAGGTGAGGAGGTGCGCAGTGGGAACGAGGAGATGCTCTCGCAGCACAGTGACAGCGTCATGGAGTACGGACCAAAGAAGCCGAGGCCAGGATTTTCTACGTGCAGAAAGGCACCTTACAGGTCCCATTCACTGTCGCCATCTTCAGTGAACAAACACAGACAATTAGAGAAAGAGAAAAAAAGGCAGCACAAATCTAAAGGGACAGACTCATGCCATTTCCAAGCAAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
ACTGTCGCCATCTTCAGTGA
59.03
CATTGTGGGCCTCGTCTTCT
60.04
145
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TGTCGCCATCTTCAGTGAACA
59.93
CATTGTGGGCCTCGTCTTCT
60.04
143
PCR template sequence:
ACTGTCGCCATCTTCAGTGAACAAACACAGACAATTAGAGAAAGAGAAAAAAAGGCAGCACAAATCTAAAGGGACAGACTCATGCCATTTCCAAGCAAAGACCTCATAGTTCTTCCCAGGAATCAAGAAGACGAGGCCCACAATGTCCAAGCGGTAATTGATTCACTCGCTTTGGATTACCTTCAGGTCAGCCTGTCTCA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
9
Interacting miRNAs (#binding sites)
mmu-miR-429-3p(1), mmu-miR-200b-3p(1), mmu-miR-200c-3p(1), mmu-miR-18a-5p(1), mmu-miR-185-5p(1), mmu-miR-1198-5p(1), mmu-miR-190b-5p(1), mmu-miR-335-3p(1), mmu-miR-202-3p(1)
siRNAs for circular RNA silencing
Junction sequence
TTTCCAAGCAAAGACCTCATAGTTCT
siRNA sets
TATGAGGTCTTTGCTTGGA, AGAACTATGAGGTCTTTGC, GAACTATGAGGTCTTTGCT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCCEP95_1163_ORF_1
0.9053
0.141372279
0.995904524
1182
ORF sequence:
ATGCCATTTCCAAGCAAAGACCTCATAGTTCTTCCCAGGAATCAAGAAGACGAGGCCCACAATGTCCAAGCGGTAATTGATTCACTCGCTTTGGATTACCTTCAGGTCAGCCTGTCTCACATAACAGGAGAAAACATAGTGAAAGGGGATAATGAATCTATCAGGAATCTCCTAGAAATATTTGATGGATTGCTGGATTATCTGACAGAACACATCAGTGAATCGTCTCCCAACAAAAGTGAAACTGAGCAGTATTCTAAAGATTCTCATGGAGAAGAAGCTGGGGAGGACCTAGAACGTACCGAGGAGGCGAAGTGGAGAAACGCTTCTTTTATGAGGTGTTCCTTCTCATCGGACACTTTGGGCCCCACCTGGGATGAAGACGAAGCTGAGTCCACAGGTGAAATAATTAGGCTGGGAGACACTGCACATACCTTTTCCCAAAGAAGTAATGCATCAGAAACTCCTCCTATGAGCATGGTTCCAAGTGCCAGGAAGCTAGGCGAGCCGATCCGAGCTGCCATTCCTTTACATCCTCCCTACCATCCTTCTGAGCCTAGAGCACCATGTCCCATAGGCAAAGAATACCTCTGGTCAAGTCGCTACTTGTCTACGCCGACTTCTGGAGAGCACATGGCACCTTCTGTGGAACCAGATGATGCTTTCTTAACTTCTACCTTATTTAAAGATGATGATCAGGAAACATATCTACCTAAACCTGAAGCCACCAGGACAAGGAAGCCTTCTAAAGGAGAAAGAGATGAAAACAGAGCTGCGATCCCCTCAGAGCATCTGCCTTTCTCTCAGAAGGCCAGGAAGCCGTTAACAGAACAAGAACTGCACGCCATGTCAGAGAAACTCTCTCAGCGGCTCTCTGAACTAGACTGGATGTTAAAAACTGCCCTGGGTGATCGAGGAACGGGCGAAACTGATGGTAATGATGGTGATGGCGGAGGTGAGGAGGTGCGCAGTGGGAACGAGGAGATGCTCTCGCAGCACAGTGACAGCGTCATGGAGTACGGACCAAAGAAGCCGAGGCCAGGATTTTCTACGTGCAGAAAGGCACCTTACAGGTCCCATTCACTGTCGCCATCTTCAGTGAACAAACACAGACAATTAGAGAAAGAGAAAAAAAGGCAGCACAAATCTAAAGGGACAGACTCATGCCATTTCCAAGCAAAG
Peptide sequence:
MPFPSKDLIVLPRNQEDEAHNVQAVIDSLALDYLQVSLSHITGENIVKGDNESIRNLLEIFDGLLDYLTEHISESSPNKSETEQYSKDSHGEEAGEDLERTEEAKWRNASFMRCSFSSDTLGPTWDEDEAESTGEIIRLGDTAHTFSQRSNASETPPMSMVPSARKLGEPIRAAIPLHPPYHPSEPRAPCPIGKEYLWSSRYLSTPTSGEHMAPSVEPDDAFLTSTLFKDDDQETYLPKPEATRTRKPSKGERDENRAAIPSEHLPFSQKARKPLTEQELHAMSEKLSQRLSELDWMLKTALGDRGTGETDGNDGDGGGEEVRSGNEEMLSQHSDSVMEYGPKKPRPGFSTCRKAPYRSHSLSPSSVNKHRQLEKEKKRQHKSKGTDSCHFQAK
IRESfinder result
IRESfinder score
0.482608795
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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