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mmu_circCep76_714
Basic information of circular RNA
PanCircBase ID:
mmu_circCep76_714
mm39_Chr. Position ID:
chr18|67762888|67771411|-
Gene name:
Cep76
Transcript:
ENSMUST00000097542.4
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
5
Exon/intron indexes:
7,6,5,4,3
Exon/intron offsets:
0,3632,4936,7320,8447
Genomic length:
8523
Exon/intron sizes in nt:
129,98,186,225,76
Flanking exon/introns:
chr18:67759778-67762888|chr18:67771411-67773077
Splice length:
714
Other database IDs:
CIRCpedia: MMU_CIRCpedia_226360, circAtlas: mmu-Cep76_0007
Human Ortholog IDs:
Mature spliced Sequence:
GATAGTGTTGATCAAGAACTCCCTTCTTCTCCAAAGCAAACCGTTGGTTTTGATAAGCAGTCAACATTAAAAAAAACTAATGTTGATCCAACACGGAGGTATCTTTACCTTCAGGTTCTGGGTGGAAAAGCTTTCTTGGAACATCTGCAAGAACCTGAGCCTTTGCCTGGACAGATTTGCTCGACTTTCACTTTATGTTTGCACTATCGGAACCAACGATTTCGCTCCAAACCTGTACCCTGCGCTTGTGAGCCAGATTTCCATGACGGCTTTTTGCTTGAAGTTCACAGAGAAAGTTTAGGTGATGGAACTAGAATGGCTGATTCAACAACAATGTTATCAATAAGTGATCCAATTCACATGGTACTAATCAAAACAGACATATTTGGTGAGACCACTTTAGTTGCGTCCTATTTCCTGGAATGGAGATCAGTTTTGGGCTCAGAAAATGGAGTGACCAATCTGACTGTGGAGCTCATGGGTGTAGGCACAGAATCAAAAGTTTCCGTGGGAATTTTAAATATAAAACTTGAAATGTACCCACCGCTCAGCCAAACGCTGTCTCAGGAGGTAGTGAACACACAGCTTGCCTTGGAACGTCAGAAAACTGCAGAGAAAGAGCGATTATTTCTCGTCTATGCTAAGCAGTGGTGGAGGGAGTATTTGCAAATTCGACCTTCTCACAACTCAAGGCTGGTCAAGATTTTTGCCCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
ATGCTAAGCAGTGGTGGAGG
59.75
TGACTGCTTATCAAAACCAACGG
59.75
140
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
ATGCTAAGCAGTGGTGGAGG
59.75
TTGACTGCTTATCAAAACCAACGG
60.26
141
PCR template sequence:
GAAAGAGCGATTATTTCTCGTCTATGCTAAGCAGTGGTGGAGGGAGTATTTGCAAATTCGACCTTCTCACAACTCAAGGCTGGTCAAGATTTTTGCCCAGGATAGTGTTGATCAAGAACTCCCTTCTTCTCCAAAGCAAACCGTTGGTTTTGATAAGCAGTCAACATTAAAAAAAACTAATGTTGATCCAACACGGAGGT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
10
Interacting miRNAs (#binding sites)
mmu-miR-140-5p(1), mmu-miR-130a-3p(1), mmu-miR-19a-3p(1), mmu-miR-24-3p(1), mmu-miR-136-5p(1), mmu-miR-130b-3p(1), mmu-miR-301b-3p(1), mmu-miR-721(1), mmu-miR-301a-3p(1), mmu-miR-539-5p(1)
siRNAs for circular RNA silencing
Junction sequence
GATTTTTGCCCAGGATAGTGTTGATC
siRNA sets
GATCAACACTATCCTGGGC, ATCAACACTATCCTGGGCA, ACTATCCTGGGCAAAAATC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCCEP76_714_ORF_1
0.7919
0.014325234
0.986433552
1113
ORF sequence:
ATGGCTGATTCAACAACAATGTTATCAATAAGTGATCCAATTCACATGGTACTAATCAAAACAGACATATTTGGTGAGACCACTTTAGTTGCGTCCTATTTCCTGGAATGGAGATCAGTTTTGGGCTCAGAAAATGGAGTGACCAATCTGACTGTGGAGCTCATGGGTGTAGGCACAGAATCAAAAGTTTCCGTGGGAATTTTAAATATAAAACTTGAAATGTACCCACCGCTCAGCCAAACGCTGTCTCAGGAGGTAGTGAACACACAGCTTGCCTTGGAACGTCAGAAAACTGCAGAGAAAGAGCGATTATTTCTCGTCTATGCTAAGCAGTGGTGGAGGGAGTATTTGCAAATTCGACCTTCTCACAACTCAAGGCTGGTCAAGATTTTTGCCCAGGATAGTGTTGATCAAGAACTCCCTTCTTCTCCAAAGCAAACCGTTGGTTTTGATAAGCAGTCAACATTAAAAAAAACTAATGTTGATCCAACACGGAGGTATCTTTACCTTCAGGTTCTGGGTGGAAAAGCTTTCTTGGAACATCTGCAAGAACCTGAGCCTTTGCCTGGACAGATTTGCTCGACTTTCACTTTATGTTTGCACTATCGGAACCAACGATTTCGCTCCAAACCTGTACCCTGCGCTTGTGAGCCAGATTTCCATGACGGCTTTTTGCTTGAAGTTCACAGAGAAAGTTTAGGTGATGGAACTAGAATGGCTGATTCAACAACAATGTTATCAATAAGTGATCCAATTCACATGGTACTAATCAAAACAGACATATTTGGTGAGACCACTTTAGTTGCGTCCTATTTCCTGGAATGGAGATCAGTTTTGGGCTCAGAAAATGGAGTGACCAATCTGACTGTGGAGCTCATGGGTGTAGGCACAGAATCAAAAGTTTCCGTGGGAATTTTAAATATAAAACTTGAAATGTACCCACCGCTCAGCCAAACGCTGTCTCAGGAGGTAGTGAACACACAGCTTGCCTTGGAACGTCAGAAAACTGCAGAGAAAGAGCGATTATTTCTCGTCTATGCTAAGCAGTGGTGGAGGGAGTATTTGCAAATTCGACCTTCTCACAACTCAAGGCTGGTCAAGATTTTTGCCCAG
Peptide sequence:
MADSTTMLSISDPIHMVLIKTDIFGETTLVASYFLEWRSVLGSENGVTNLTVELMGVGTESKVSVGILNIKLEMYPPLSQTLSQEVVNTQLALERQKTAEKERLFLVYAKQWWREYLQIRPSHNSRLVKIFAQDSVDQELPSSPKQTVGFDKQSTLKKTNVDPTRRYLYLQVLGGKAFLEHLQEPEPLPGQICSTFTLCLHYRNQRFRSKPVPCACEPDFHDGFLLEVHRESLGDGTRMADSTTMLSISDPIHMVLIKTDIFGETTLVASYFLEWRSVLGSENGVTNLTVELMGVGTESKVSVGILNIKLEMYPPLSQTLSQEVVNTQLALERQKTAEKERLFLVYAKQWWREYLQIRPSHNSRLVKIFAQ
IRESfinder result
IRESfinder score
0.712427487
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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