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mmu_circCep70_863
Basic information of circular RNA
PanCircBase ID:
mmu_circCep70_863
mm39_Chr. Position ID:
chr9|99163080|99180635|+
Gene name:
Cep70
Transcript:
ENSMUST00000093795.10
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
7
Exon/intron indexes:
11,12,13,14,15,16,17
Exon/intron offsets:
0,8168,10497,14999,15248,16551,17475
Genomic length:
17555
Exon/intron sizes in nt:
75,106,171,147,169,115,80
Flanking exon/introns:
chr9:99160251-99163080|chr9:99180635-99181780
Splice length:
863
Other database IDs:
CIRCpedia: HSA_CIRCpedia_369246
Human Ortholog IDs:
Mature spliced Sequence:
GCCCACAGAACATGAATTAAGACTTTATAAACAACAAGTGAAGAAACTGGAAAAAACCCTGAAGAAAAACATAAAATTACAAGATCTTATTGGTCAAAAAAAGTCTGATGACACGGAGAAAAAAGATGAGCCCAGCAAAGACAGCCATCAGCAGGCTCTCATTGAGCAGAGCTACTTTCAGGTGCTGTGCAGCATCAATTCAATTGTTCACAACCCACGAGCTCCAGTGATTATTTATAAACAGAGCAAAGGAAGAGCCCCAAACGGCAATAAAGATATTGGTCAAGATTGTGGATTTGAACATCTTGTTCCTATAATAGAAATGTGGGTAGATGAACTGACATCCTTAAAGGATTTGTATAAGTCCTTGAAAATACTGTCTGCAGAACTGGTGCCTTGGCATAGCTTAAAGAAACTGGATGAAAAGGAAGGTGTCAAAGTCGGAGATCTGTTGTTTATGGTGGATACCATGTTGGAAGAAGTTGAAAACCAGAAAGAGACCAGCAGCACGCCGAACTCTCAAACTTTGCAAGCTATCGTTTCTCACTTCCAAAAGCTATTTGATGTGCAGTCTTTAAATGGAGTCTTTCCCCGGATGAATGAAGTGTACACCAGGCTTGGAGAAATGAACAACGCTGTAAGGAACCTTCAGGAGCTCTTAGAATTAGACAGTTCATCATCATTGTGTGTGGTGGTCAGCACAGTTGGTAAACTGTGTGAGATAATTAACAAGGATGTGAGTGAGCAGGTTAAACAAGTGTTAGGACCTGAGGATCTACAAAGTATTATCAAGAAATTGGAAGAGCATGAGGAATTTTTCCCAGCGTTTCAAGCTTTTGCTAATGATCTTCTTGAAATCTTAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GGACCTGAGGATCTACAAAGT
56.45
TCCAGTTTCTTCACTTGTTGT
55.57
151
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GGACCTGAGGATCTACAAAGT
56.45
TCCAGTTTCTTCACTTGTTGTT
56.33
151
PCR template sequence:
GGACCTGAGGATCTACAAAGTATTATCAAGAAATTGGAAGAGCATGAGGAATTTTTCCCAGCGTTTCAAGCTTTTGCTAATGATCTTCTTGAAATCTTAGGCCCACAGAACATGAATTAAGACTTTATAAACAACAAGTGAAGAAACTGGAAAAAACCCTGAAGAAAAACATAAAATTACAAGATCTTATTGGTCAAAAA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
3
Interacting miRNAs (#binding sites)
mmu-miR-223-3p(1), mmu-miR-342-3p(1), mmu-miR-369-3p(1)
siRNAs for circular RNA silencing
Junction sequence
CTTGAAATCTTAGGCCCACAGAACAT
siRNA sets
ATGTTCTGTGGGCCTAAGA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCCEP70_863_ORF_1
0.9406
0.108469818
0.722635503
561
ORF sequence:
ATGTGGGTAGATGAACTGACATCCTTAAAGGATTTGTATAAGTCCTTGAAAATACTGTCTGCAGAACTGGTGCCTTGGCATAGCTTAAAGAAACTGGATGAAAAGGAAGGTGTCAAAGTCGGAGATCTGTTGTTTATGGTGGATACCATGTTGGAAGAAGTTGAAAACCAGAAAGAGACCAGCAGCACGCCGAACTCTCAAACTTTGCAAGCTATCGTTTCTCACTTCCAAAAGCTATTTGATGTGCAGTCTTTAAATGGAGTCTTTCCCCGGATGAATGAAGTGTACACCAGGCTTGGAGAAATGAACAACGCTGTAAGGAACCTTCAGGAGCTCTTAGAATTAGACAGTTCATCATCATTGTGTGTGGTGGTCAGCACAGTTGGTAAACTGTGTGAGATAATTAACAAGGATGTGAGTGAGCAGGTTAAACAAGTGTTAGGACCTGAGGATCTACAAAGTATTATCAAGAAATTGGAAGAGCATGAGGAATTTTTCCCAGCGTTTCAAGCTTTTGCTAATGATCTTCTTGAAATCTTAGGCCCACAGAACATGAATTAA
Peptide sequence:
MWVDELTSLKDLYKSLKILSAELVPWHSLKKLDEKEGVKVGDLLFMVDTMLEEVENQKETSSTPNSQTLQAIVSHFQKLFDVQSLNGVFPRMNEVYTRLGEMNNAVRNLQELLELDSSSSLCVVVSTVGKLCEIINKDVSEQVKQVLGPEDLQSIIKKLEEHEEFFPAFQAFANDLLEILGPQNMN
IRESfinder result
IRESfinder score
0.661456223
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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